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The map label for this gene is braB [H]

Identifier: 226950719

GI number: 226950719

Start: 3744057

End: 3745397

Strand: Reverse

Name: braB [H]

Synonym: CLM_3727

Alternate gene names: 226950719

Gene position: 3745397-3744057 (Counterclockwise)

Preceding gene: 226950720

Following gene: 226950718

Centisome position: 90.14

GC content: 33.93

Gene sequence:

>1341_bases
ATGTCAGAACAAAAAAGACGAAAGGATATTTTTGTACAAGGATTCGCACTATTCGCAATTTTTTTCGGAGCAGGAAACTT
AATTTTTCCACCTAGTTTAGGAATGGCCGCAGGAGATAATTGGGCTCTGGCTGCTTTTGGTTTTTTACTTACAGATCCGG
TACTGCCGATCTTAGGTGTAATTGCAACAGCTAAGGTAGGGGGGAAAGCAGAAGACTTAGGTAAGAGAGTTAGTCCAGGC
TTTGCAAAGGTTTTAGGAGCTGTCTGTATTTTGGTTATTGGTCCTTTATTCGCTATTCCTAGAACAGCGGCTACTGTATA
TGAAATAGCAGTAAAACCAGTAGCACCTGGTGTACCAATCGCACCTGGTGTACCAATCATAGTTATATCACTAATATTTT
TTGTGTTAACCTATATATTTGTAAGTAATCAGGGGCATGTAATAGATAATATCGGTAAAATTTTAACACCATTGTTATTA
ATTATACTAGCTGCCATAGTAATTGCAAGTATAGTAACACCTGTAGGGCCAATAGTAGCAACAAAACCTAATAGATACTT
TTTAATAGGTTTCCAGGAAGGGTATCAAACAATGGATGCATTGGGAGCATCATTAATGGCTGGAATAGTATTAACAGATT
TAATTTTAAAAGGATATAAAGATAGAAAAGAACAAGAATATATGACTATTAGGGTAGGCTTAGTTTCAGGAGTTCTATTA
TTATTGGTATATGGAGGTCTTACATTTGTAGGAGCTACTGGAAGTGGAATTTTTAGCGGAGATATTAGTAGAGTTGATCT
ATTATTAAAATTAGTATATGGACTTTTCGGAAATGCTGGTGCTGTAGCTATATCCGTAGCAGTAGCTTTAGCTTGTTTAA
CTACAGCAGTAGGACTTACTTCTACAGCAGCTAATTTTTTTAATAGTGTAAGTAAGGGGAAGCTTTCTTACAAGGCTGTT
AGTATTGCTGTAGTATTATGTAGTTTATTTTTATCTGTAATAGGGGTAGAAGGATTAATCAATATAGCTGTACCAATTTT
ATCTACTGTATACCCAGTCATGATTGTTTTAATATTAATGACATTATTTGATAGACACATTCCTAATAATTTAACTTATA
CCGGTGCCGTAATAGGAGCATTTTTAGTTAGTTTAATTATAAATATAAATAGTGCATTTGGAATTTTAAATGGTCCAATG
GCGCTTGTGAAAAAATTACCTCTTTTTGAAGCGGGATTTCCATGGATTGTACCAGCTATAGTATTAGCTATTTTGTTCAC
TATATTTGGGAAAAAAAATAAATTCCAAAGCTTAGAACATGAAAACTCTTTTGATAATTAA

Upstream 100 bases:

>100_bases
TAAATTTTATTGAAATTCTGAATAATTGCTATATTGACTACAATTTATTTATGTGATACATTGATAAAGAATAAACAATT
TAAAAAAAGGAGTGTGAAAA

Downstream 100 bases:

>100_bases
CCTACGTTGATCTGCTAAATCGTAAAAGTGATAAAATAGTAATGAAAAAAGCAATTATAGAGATATTAAAATTTTATGGA
AAATTATAGGCAGATAGTTT

Product: branched-chain amino acid transport system II carrier protein

Products: L-leucine [Cytoplasm]; L-valine [Cytoplasm]; Na (I) [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: Branched-chain amino acid uptake carrier BraB [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPG
FAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLL
IILAAIVIASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLL
LLVYGGLTFVGATGSGIFSGDISRVDLLLKLVYGLFGNAGAVAISVAVALACLTTAVGLTSTAANFFNSVSKGKLSYKAV
SIAVVLCSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPM
ALVKKLPLFEAGFPWIVPAIVLAILFTIFGKKNKFQSLEHENSFDN

Sequences:

>Translated_446_residues
MSEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPG
FAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLL
IILAAIVIASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLL
LLVYGGLTFVGATGSGIFSGDISRVDLLLKLVYGLFGNAGAVAISVAVALACLTTAVGLTSTAANFFNSVSKGKLSYKAV
SIAVVLCSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPM
ALVKKLPLFEAGFPWIVPAIVLAILFTIFGKKNKFQSLEHENSFDN
>Mature_445_residues
SEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGVIATAKVGGKAEDLGKRVSPGF
AKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPIAPGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLI
ILAAIVIASIVTPVGPIVATKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLLL
LVYGGLTFVGATGSGIFSGDISRVDLLLKLVYGLFGNAGAVAISVAVALACLTTAVGLTSTAANFFNSVSKGKLSYKAVS
IAVVLCSLFLSVIGVEGLINIAVPILSTVYPVMIVLILMTLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPMA
LVKKLPLFEAGFPWIVPAIVLAILFTIFGKKNKFQSLEHENSFDN

Specific function: Component of the transport system for branched-chain amino acids (leucine, isoleucine and valine) Which is coupled to a proton motive force [H]

COG id: COG1114

COG function: function code E; Branched-chain amino acid permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the branched chain amino acid transporter family [H]

Homologues:

Organism=Escherichia coli, GI1786601, Length=448, Percent_Identity=33.9285714285714, Blast_Score=224, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004685 [H]

Pfam domain/function: PF05525 Branch_AA_trans [H]

EC number: NA

Molecular weight: Translated: 47059; Mature: 46928

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SEQKRRKDIFVQGFALFAIFFGAGNLIFPPSLGMAAGDNWALAAFGFLLTDPVLPILGV
CCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
IATAKVGGKAEDLGKRVSPGFAKVLGAVCILVIGPLFAIPRTAATVYEIAVKPVAPGVPI
HHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCC
APGVPIIVISLIFFVLTYIFVSNQGHVIDNIGKILTPLLLIILAAIVIASIVTPVGPIVA
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
TKPNRYFLIGFQEGYQTMDALGASLMAGIVLTDLILKGYKDRKEQEYMTIRVGLVSGVLL
ECCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCCHHHHHHHHHHHHHHHHH
LLVYGGLTFVGATGSGIFSGDISRVDLLLKLVYGLFGNAGAVAISVAVALACLTTAVGLT
HHHHHCHHEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
STAANFFNSVSKGKLSYKAVSIAVVLCSLFLSVIGVEGLINIAVPILSTVYPVMIVLILM
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLFDRHIPNNLTYTGAVIGAFLVSLIININSAFGILNGPMALVKKLPLFEAGFPWIVPAI
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHH
VLAILFTIFGKKNKFQSLEHENSFDN
HHHHHHHHHCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-leucine [Periplasm]; L-valine [Periplasm]; Na (I) [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-leucine [Periplasm] = Na (I) [Cytoplasm] + L-leucine [Cytoplasm] Na (I) [Periplasm] + L-valine [Periplasm] = Na (I) [Cytoplasm] + L-valine [Cytoplasm] Na (I) [Periplasm] + L-iso-leucine [Periplasm] = Na (I) [Cytoplasm] + L-iso-leuci

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]