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The map label for this gene is tig [H]

Identifier: 226950660

GI number: 226950660

Start: 3684068

End: 3685360

Strand: Reverse

Name: tig [H]

Synonym: CLM_3644

Alternate gene names: 226950660

Gene position: 3685360-3684068 (Counterclockwise)

Preceding gene: 226950661

Following gene: 226950659

Centisome position: 88.69

GC content: 28.69

Gene sequence:

>1293_bases
ATGAACGTTAAAGTGGAAAATATAGAAAAGAATGTTGTAAAATTAGAAATAACAGTTGATAGTGAAAAATTCAATGAAGC
TGTTAAAAAATCATTTAAAAAGAATGCTAAAAGATTCAATGTACCAGGATTTAGAAAAGGTAAAGCACCTTTAAATATAA
TAAAAAAATACTATGGGGAAGGTGTTTTATTTGAAGATGCTATAAATTTCTGTTGTGAAGATACATATCCTAAAGCCATA
GAAGAAAATAACATAAAACCAGTAGATTATCCTCAAATAGATGTAGTTCAAATAGGAGAAGGAAAAGATTTCATATATAC
AGCAGAAGTTACAACTGTTCCTAAGGTTAAACTAGGAGAATATAAGGGCGTAGAAGTTAAAAAAGTTTCATATGAAGTAG
AAGATGAAGCTGTAGAAAATGAATTAAAATCCATGCAAGAAAAAAATGCAAGAGTATCTCTAAAAGAAGAAGGGGAAATT
GAAAAAGGAAATATAGCAATAATTGATTTTAAAGGATATGTAGATGGAAAAGCTTTTGAAGGTGGAGAGGCTAAGGATTA
TGAAATAGAAATAGGTTCAGGCACTTTCATAGGAGATTTTGAAGATCAATTAGTAGGTCTTAAAAAAGATGAATCAAAAG
AAGTAAATGTAAGTTTCCCAGAAGAATATGGTAGAGAAGATTTAAATGGAAAACCAGCTACTTTTGAAGTAACAATTAAA
GATATAAAGGTTAAAGAGCTTCCAGCATTAGATGATGAATTTGCAAAAGAAGTTTCAGAATTTGATACATTAGAGGAATT
AAAATCAGATATAAAGGATAGAATGAAAAAAGAACTATCTGAAAAAGCTAAAGCAGAATATGAAGAAGCAGTAGTTGAAG
CAGTTGGGGCAAATGCTGAAATAGAAATACCAAAAGTTATGATTGAAAAAGAAATAGAAAATATGGTAAGAGATTTAGAA
ATGAGATTAAAATACCAAGGCTTAGACCTAAAATCATATTATGAATTTACAAATAGTTCAGAAGAGAAAGTAAAAGAATA
TATGAGAGAAACAGCTGAAAAAAGAGTTAAAACAGATTTAATAATGCAAGAAATTGCTAAAGTAGAAGATATAAAAGCTA
CAGAAGAGGAATTAAAAGAAAAAGCTATGGAAGTAGCTAAACAATATGGTCAAAAAGATGTAGAAAAAACTGCTGAGTTA
ATAGCAAATGCTCAAAAAGCATACTTAGAAATTGATATAGTTAATGGAAAAGTTTTAGACCTATTGGTAGAGAGTAGCAA
AGAAATAGCTTAG

Upstream 100 bases:

>100_bases
TACTTTATGTTAGCGTAATGTATGTTAATAGTTCTAAATATATGATATAATGATATAGTTAAAATGAGCATATAACAAGG
AATTGGGAGGAAGAAGTATA

Downstream 100 bases:

>100_bases
TTCATTTATAATAATAGATAATGATATAGTTGCCAGGATATATTTTCTGGCAACTAAGTTTTAATAATTATAAATATCAC
ATAGGCATCATTAGTATCTT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPKVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA

Sequences:

>Translated_430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPKVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA
>Mature_430_residues
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGEGVLFEDAINFCCEDTYPKAI
EENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPKVKLGEYKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEI
EKGNIAIIDFKGYVDGKAFEGGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAEIEIPKVMIEKEIENMVRDLE
MRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDLIMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAEL
IANAQKAYLEIDIVNGKVLDLLVESSKEIA

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=417, Percent_Identity=31.6546762589928, Blast_Score=182, Evalue=4e-47,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 48924; Mature: 48924

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGE
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLFEDAINFCCEDTYPKAIEENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPKVKLGE
CEEHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEECCC
YKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEIEKGNIAIIDFKGYVDGKAFE
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
GGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
CCCCCCEEEEECCCCEECCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEEEEE
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IEIPKVMIEKEIENMVRDLEMRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDL
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAELIANAQKAYLEIDIVNGKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
LLVESSKEIA
HHHHCCCCCC
>Mature Secondary Structure
MNVKVENIEKNVVKLEITVDSEKFNEAVKKSFKKNAKRFNVPGFRKGKAPLNIIKKYYGE
CCCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCC
GVLFEDAINFCCEDTYPKAIEENNIKPVDYPQIDVVQIGEGKDFIYTAEVTTVPKVKLGE
CEEHHHHHHHHHCCCCCCHHHCCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCEECCC
YKGVEVKKVSYEVEDEAVENELKSMQEKNARVSLKEEGEIEKGNIAIIDFKGYVDGKAFE
CCCCEEEEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCC
GGEAKDYEIEIGSGTFIGDFEDQLVGLKKDESKEVNVSFPEEYGREDLNGKPATFEVTIK
CCCCCCEEEEECCCCEECCCHHHHCCCCCCCCCCEEEECCHHHCCCCCCCCCCEEEEEEE
DIKVKELPALDDEFAKEVSEFDTLEELKSDIKDRMKKELSEKAKAEYEEAVVEAVGANAE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IEIPKVMIEKEIENMVRDLEMRLKYQGLDLKSYYEFTNSSEEKVKEYMRETAEKRVKTDL
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
IMQEIAKVEDIKATEEELKEKAMEVAKQYGQKDVEKTAELIANAQKAYLEIDIVNGKVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCEEEEEEEECCHHHH
LLVESSKEIA
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA