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The map label for this gene is fliF [H]

Identifier: 226950075

GI number: 226950075

Start: 3027002

End: 3028582

Strand: Reverse

Name: fliF [H]

Synonym: CLM_3028

Alternate gene names: 226950075

Gene position: 3028582-3027002 (Counterclockwise)

Preceding gene: 226950076

Following gene: 226950074

Centisome position: 72.89

GC content: 31.12

Gene sequence:

>1581_bases
ATGAATAAGCTAAAGGAGTTTTTTAAAGGATTAAAAGAAAAGTGGACAGGGTTTAGTAAAGTAAAGAAAATAGCTTTTTC
TATAATTTTTTTAGGAATTATAACATCTATTATTGCATTATCTCTTTATTTTGGAAAAACTAAGTATGCAGTTTTATTTT
CCAATATGGATTCTAATGATTCTGGAACGGTACTTCAAAAATTAAAGGATAAAAAAGTTGAAGCTAAGGTAGAGGGAAAT
AATATATTAGTACCTAAAGATAAGGTTGATGAACTAAGGATGCAAATGTTATCTGAAGTTCCATTAACTAATGGTAGTCA
AGGATTTGAAATATTAGATAAAAGCCAGTTTGGAGAAACAGATCAGGAAATGAAAATAAACTATCAAAGAGCCCTTCAAG
GAGAATTAGAACGAACTATAAAAGGGTTTCCGCAAGTGGATAATACCAGAGTTCATTTAGTTCTTCCTGAAGAAACTGCC
TTTGTAAAGGAAACACAGCCAGGTAGAGCTTCTGTTACTTTAAACTTAAAACAAGGACAAAATCTTAGTAAGGAGCAAGT
TAAATCTATAGTAGCTTTAGTAAGCGGAAGTGTGAAAAATATTCCGAAAGAAAATGTAGAAGTAATTGATAACAACATGA
ATCTTCTTACAAAGAATTTATTTGATGCTTCAGGAAGTTTAGAAGAGGCTACAACTTCAGCAGAAAAACAGCAACAACTT
CAAAAAAATTATGAGAAAGATCTTCAGAATAGACTAGTTAGTATGTTGGAAGCTGTATATGGAAAGGACAAGGTTAAAGT
TAATATTAACACAGATTTAGATTTTGATGCAGTTAAAACTAATTCTGTAACCTATGATCCTAAAAATGTAGTAGTTAGTG
AACATAGCATTAAAGAGAAAAATCAGAATAATGCAGGTGGAAATAATACAAATGGTAGTGTAGTAGATAATAATATGGTA
AATAGAACTACCCAAAATAATAATGGTAGTGAGACATCTTCTAGAGATGAAAATACTAAAAATTATGAAATATCAAAAAC
CCAGCAGGATAGTATAAAGGCCCCAGGCTCCGTAAAAAGATTAACAGCGTCTATTGTGTTAGATGGAAATATAGATGAAG
AAACTAGAAGTGCTGTTAGAAATTTAGCTGTATCTGCTATAGGTTATGATGAAAAAAGAGGAGATACCATAAACGTAGAG
GGTCTTCCATTTGATACAGCGGCTAAGGATAAAGTGAAAAAAGATTTAGAAGATATGCAAAAAACTGAAAAAACAAAAGA
AAGAATAAAATTGTTTACTGCTATAGGATTAGGTGTATTACTGTTATTAGGAGCAATAATATTCTTTATTATTAAGAAGC
GAAACAACGATGAAGAGTATGAGGAAGATGAAGAAGGTCTTGATATTTTAATAGATGATAACGACAGTGAAACTAAACAG
GTTCCTAAGTTTAAACCTATAGATTTAGAAACACAGGATGAAAAGACCCATGTTGAAAATGAAATAAGAAAATATGCTAA
GGACAAACCTGATCAAGTGGCGGAAATAATTAAATCATGGCTAGCAGAGGATGAGAGGTGA

Upstream 100 bases:

>100_bases
AGCTAAAATGTCCATGGAGCTAGCAGTTCAGGTTAGAAACAAGCTAGTAGAAGCTTATCAAGAATTAAGTAGAATGCAAT
TATAGTAAGGGGAGCTTTCG

Downstream 100 bases:

>100_bases
TAATATATGGCTAAAGATGATAAATTAACAGGAGTTCAAAAAGCGGCAATACTATTTATTACTTTAGGTCCTGAGGCCTC
TGCTGGTATAATAAAAAAAT

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQNLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKTEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNNDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLETQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER

Sequences:

>Translated_526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQNLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKTEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNNDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLETQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
>Mature_526_residues
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSNDSGTVLQKLKDKKVEAKVEGN
NILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGETDQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETA
FVKETQPGRASVTLNLKQGQNLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEKNQNNAGGNNTNGSVVDNNMV
NRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKRLTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVE
GLPFDTAAKDKVKKDLEDMQKTEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNNDEEYEEDEEGLDILIDDNDSETKQ
VPKFKPIDLETQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=423, Percent_Identity=29.7872340425532, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59103; Mature: 59103

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSND
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
SGTVLQKLKDKKVEAKVEGNNILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGET
CHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCHHHCCCC
DQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETAFVKETQPGRASVTLNLKQGQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCC
NLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
CCCHHHHHHHHHHHHCCHHCCCCCCHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEK
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEECCCCEEECCHHHHHC
NQNNAGGNNTNGSVVDNNMVNRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKR
CCCCCCCCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCCCHHHH
LTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVEGLPFDTAAKDKVKKDLEDMQ
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KTEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNNDEEYEEDEEGLDILIDDNDSETKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCHHHC
VPKFKPIDLETQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNKLKEFFKGLKEKWTGFSKVKKIAFSIIFLGIITSIIALSLYFGKTKYAVLFSNMDSND
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCC
SGTVLQKLKDKKVEAKVEGNNILVPKDKVDELRMQMLSEVPLTNGSQGFEILDKSQFGET
CHHHHHHHHCCCCEEEECCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCHHHCHHHCCCC
DQEMKINYQRALQGELERTIKGFPQVDNTRVHLVLPEETAFVKETQPGRASVTLNLKQGQ
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCHHHCCCCCCCEEEEEEECCCC
NLSKEQVKSIVALVSGSVKNIPKENVEVIDNNMNLLTKNLFDASGSLEEATTSAEKQQQL
CCCHHHHHHHHHHHHCCHHCCCCCCHHEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
QKNYEKDLQNRLVSMLEAVYGKDKVKVNINTDLDFDAVKTNSVTYDPKNVVVSEHSIKEK
HHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCCEEECCCCEEECCHHHHHC
NQNNAGGNNTNGSVVDNNMVNRTTQNNNGSETSSRDENTKNYEISKTQQDSIKAPGSVKR
CCCCCCCCCCCCCEEECCHHCCCCCCCCCCCCCCCCCCCCCEEECCCCHHCCCCCCHHHH
LTASIVLDGNIDEETRSAVRNLAVSAIGYDEKRGDTINVEGLPFDTAAKDKVKKDLEDMQ
EEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHH
KTEKTKERIKLFTAIGLGVLLLLGAIIFFIIKKRNNDEEYEEDEEGLDILIDDNDSETKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCEEEECCCCCHHHC
VPKFKPIDLETQDEKTHVENEIRKYAKDKPDQVAEIIKSWLAEDER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]