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The map label for this gene is ygfA [C]

Identifier: 226950016

GI number: 226950016

Start: 2970872

End: 2971426

Strand: Reverse

Name: ygfA [C]

Synonym: CLM_2969

Alternate gene names: 226950016

Gene position: 2971426-2970872 (Counterclockwise)

Preceding gene: 226950017

Following gene: 226950015

Centisome position: 71.51

GC content: 26.31

Gene sequence:

>555_bases
ATGAATAATAAAGCTATTTTAAGAAAAGAAATTATAAATGTAAGGAAATCTTTACCTTTAGAAAAAAAAACACAAAAAGA
TATAATAATAAAAGATAAGCTCCAGGAAAATTCTTTGTATAAAGAATCTAATTTCATATTTATATACGTAAGTTTAAAAG
ATGAAGTGGACACACATGCTATTATAAAAGAAGGATTAAAGAATGGAAAAAGAATATGCGTACCTAAAGTTATATCTATA
CAAGAAGGTATGGTGGCTATAGAAATAAAGGATTTTTCAGAACTTATAGAATGTGGACCTTATAAAATATTAGAACCTAA
AAATTTTAAAAATATAGTAGATTCAAAAGAAATAGATCTAGCTATTTTACCAGGTTTAGCTTTTGATAGCAAAGGAGGGA
GATTAGGTTATGGTGGAGGCTTTTATGATAGGTTTATTCCTAAACTAAAAAAAGAAGTCTCAAAAATAGCCTTAGCCTAT
GATTTTCAAATATTAAAAGAGGTTCCTAAAGATGCACATGATGTGCTAGTAGATGAGATTATAATAGATATTTAA

Upstream 100 bases:

>100_bases
GCTGCAGCAGCAAATTATAGATATAATTTAGTTACAAAAAATTGCCCAGCAGATATAATAAATGAAAGAATTAGTAAGGA
GGCTGTTAATTCAAGAGAAG

Downstream 100 bases:

>100_bases
TATTTATAAACAGAGTTCTTGGCTTTAGAGGGAGTTTTTACTCCCTCTGAAGCTTATTAACAGAATTCTTAGGAGCTTTA
CTCCTTAGAAGTCGTTATCC

Product: 5-formyltetrahydrofolate cyclo-ligase family protein

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MNNKAILRKEIINVRKSLPLEKKTQKDIIIKDKLQENSLYKESNFIFIYVSLKDEVDTHAIIKEGLKNGKRICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDSKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDEIIIDI

Sequences:

>Translated_184_residues
MNNKAILRKEIINVRKSLPLEKKTQKDIIIKDKLQENSLYKESNFIFIYVSLKDEVDTHAIIKEGLKNGKRICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDSKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDEIIIDI
>Mature_184_residues
MNNKAILRKEIINVRKSLPLEKKTQKDIIIKDKLQENSLYKESNFIFIYVSLKDEVDTHAIIKEGLKNGKRICVPKVISI
QEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDLAILPGLAFDSKGGRLGYGGGFYDRFIPKLKKEVSKIALAY
DFQILKEVPKDAHDVLVDEIIIDI

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=191, Percent_Identity=29.8429319371728, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1789278, Length=142, Percent_Identity=28.169014084507, Blast_Score=73, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17510857, Length=190, Percent_Identity=26.8421052631579, Blast_Score=79, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6321031, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=3e-12,
Organism=Drosophila melanogaster, GI161077430, Length=192, Percent_Identity=27.6041666666667, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 20936; Mature: 20936

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKAILRKEIINVRKSLPLEKKTQKDIIIKDKLQENSLYKESNFIFIYVSLKDEVDTHA
CCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEEEEECCCCHHHH
IIKEGLKNGKRICVPKVISIQEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDL
HHHHHHCCCCEEECCEEEECCCCEEEEEEHHHHHHHHCCCCEEECCCCHHHHCCCCCCCE
AILPGLAFDSKGGRLGYGGGFYDRFIPKLKKEVSKIALAYDFQILKEVPKDAHDVLVDEI
EEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIDI
HHCC
>Mature Secondary Structure
MNNKAILRKEIINVRKSLPLEKKTQKDIIIKDKLQENSLYKESNFIFIYVSLKDEVDTHA
CCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCEECCCEEEEEEEECCCCHHHH
IIKEGLKNGKRICVPKVISIQEGMVAIEIKDFSELIECGPYKILEPKNFKNIVDSKEIDL
HHHHHHCCCCEEECCEEEECCCCEEEEEEHHHHHHHHCCCCEEECCCCHHHHCCCCCCCE
AILPGLAFDSKGGRLGYGGGFYDRFIPKLKKEVSKIALAYDFQILKEVPKDAHDVLVDEI
EEECCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IIDI
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12089438 [H]