| Definition | Clostridium botulinum A2 str. Kyoto chromosome, complete genome. |
|---|---|
| Accession | NC_012563 |
| Length | 4,155,278 |
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The map label for this gene is bacA [H]
Identifier: 226949942
GI number: 226949942
Start: 2905446
End: 2907275
Strand: Reverse
Name: bacA [H]
Synonym: CLM_2894
Alternate gene names: 226949942
Gene position: 2907275-2905446 (Counterclockwise)
Preceding gene: 226949943
Following gene: 226949941
Centisome position: 69.97
GC content: 25.9
Gene sequence:
>1830_bases ATGAGCTATAACTATTTTGAAAAACGACAAAGAGAAGATTCTATATTGGTTGGACAGGATATTTTAAGTTTTAGATATAA TAATAGCAGAATAGATACTACAATAATTTCTTGCTTAAGAAAAATCGTTAACTTAAGTAAAGAAAAAGTAGCTATTATAG ATAATGAAACAAAGATAACTTATGGACATTTCATGAAGCGAATTAATGCTCTATCAAATTTTTTGCTGAATCAAAAGGAG TTTGAAAGAACATCACCAATTACTTTAATTTTAAATGGGAATTATTTAAGATATGTTTGCGTATATGGTGTTTTAGCTTC AGGTGGGTATTATTTGCCTATAGAACCAGAAGTAAACAATATTAATAGAATAATTTCTTTAGTAGAACGTTCTAAAAGTA AAGTTGTAATTACAAATAGAGAGTATGTAAATATATTAAAAGAAAAAATATCAGAAAGTGTAAATTTTATAATTATAGAA GATTTAGATTTTAGTAGCCTAGATAGCAAAGAAAACGTTATTCATATAAAAGCTGATGATCCAGCATATATGATATATAC ATCTGGATCTACGGGAGTACCTAAAGGTGTTATTATACCTCATAGAGCTGTTGTGAATTTAGTTAATGCATTGTACGTTA ATTTTAGATTAACAAAAAAGGATAGGGGTGTGGCATTTACATCATTTTCTTTTGATTCATGTGGTTGTGATATATATCCA TATTTATTGAATGAAGTCCCTTTAGTATGCATTGAGAGGAGAAAACAATATGTTTACGATATTGAAAAATTAAATAAATT TTGTATTGATAATATGATATCAATAATGTTTTTACCTACGGTTTTAGCTGAAAAATTTATTACAGTTGATAATAATGTGC TAAGAGTTCTTTATTTTGGAGGGGAAAAATTTTGTAAGAATAAAGTTACTAATTATGAGTTATTTAATTCTTATGGATTG ACAGAAACAGGAATACTTAATACAGTCTATAAGATTAAAGGAGATGAGCGAGAAATTCCTTTGGGAGATCCAATAATAAA TTCAGGAATATTAATTTTAGATGATCAAGATAAAATTATACCTAAAGGGAAATTAGGAGAAATTGCAATTTTTGGTTCTG GTATAGCAATAGAATATGATAATAATACAGAGTTGACTAATAAAAAATTTATTAAGTTAAAAGAAATGGGTAATTTGAGA TGTTGTAAAACTGGAGATTTGGGATATATAAATGAGAATGATAATCTATATTTTAAGGGTAGAAAAGATAAACAAGTAAA AATAAGTGGTTACAGGATAGAGTTAGATGAGATACGATTTTATTTGCAGAAATATGCTTGCATAGAATTAAGTATTCCTA TGGTAATAAAAAAAGGTAAATTATCTTTATTAGTTAATTTTTATGTTGCAAAAGAAGTACTTGATGAAGAATTAATTAAT AAATTTTTAAGTAAATATTTACCTTTTTATATGGTGCCATTATGTCACATTAACATAAAAGATGTTCCATATACTACAAA TGGAAAAGTTCATTATAAGGGGTTGCAGAAAATTTTTGAGAATAAAAAAGGAAAAGTATACTTAAAAAGTAATTTACTGT ATAGCAAATTAGAATTAAAAGAGTTCCTAATTGATACTTATGCCAATATACTTAAAATGGATAAAGATGAAATAATGGAA GATGGTAGTTTCTTTGAAATGGGTGGAGACTCACTTAAAGCTATGAAATTACAATGGTATATACAAGATGAATTAGGTGT AGAAGTGGACATATCTAAAATTTATGAAGAATTCAGTATAAAAAAACTCCTAAATTTAATACTAATATGA
Upstream 100 bases:
>100_bases CTTATTTAGAAAAAAAATAGATGAAATTTTAATGATTAAAGAAATTATAAATAATAAGTAAAAATGTGAGTTATATAAAA ATAATTAGGAGAAAAAAAGG
Downstream 100 bases:
>100_bases TTATATCCAAGTTATTCTATTTTCAAGGAGGTGTAATGGTGTGAGTATAGTTCGATTATTTACATTACCAGGGGCGGGTT CTTCAGCGATAATGTATTAC
Product: putative nonribosomal peptide synthetase
Products: NA
Alternate protein names: BA1; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; ATP-dependent cysteine adenylase; CysA; Cysteine activase; ATP-dependent leucine adenylase; LeuA; Leucine activase; ATP-dependent glutamate adenylase; GluA; Glutamate activase; ATP-dependent isoleucine adenylase; IleA; Isoleucine activase; Glutamate racemase [H]
Number of amino acids: Translated: 609; Mature: 608
Protein sequence:
>609_residues MSYNYFEKRQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKE FERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNFIIIE DLDFSSLDSKENVIHIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP YLLNEVPLVCIERRKQYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN KFLSKYLPFYMVPLCHINIKDVPYTTNGKVHYKGLQKIFENKKGKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI
Sequences:
>Translated_609_residues MSYNYFEKRQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKE FERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNFIIIE DLDFSSLDSKENVIHIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP YLLNEVPLVCIERRKQYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGL TETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLR CCKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN KFLSKYLPFYMVPLCHINIKDVPYTTNGKVHYKGLQKIFENKKGKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIME DGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI >Mature_608_residues SYNYFEKRQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKITYGHFMKRINALSNFLLNQKEF ERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNNINRIISLVERSKSKVVITNREYVNILKEKISESVNFIIIED LDFSSLDSKENVIHIKADDPAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYPY LLNEVPLVCIERRKQYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFGGEKFCKNKVTNYELFNSYGLT ETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKIIPKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRC CKTGDLGYINENDNLYFKGRKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELINK FLSKYLPFYMVPLCHINIKDVPYTTNGKVHYKGLQKIFENKKGKVYLKSNLLYSKLELKEFLIDTYANILKMDKDEIMED GSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSIKKLLNLILI
Specific function: Activates five amino acids, incorporates two D-amino acids, releases and cyclizes the mature bacitracin [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 5 acyl carrier domains [H]
Homologues:
Organism=Homo sapiens, GI45580730, Length=594, Percent_Identity=24.0740740740741, Blast_Score=128, Evalue=1e-29, Organism=Escherichia coli, GI1786801, Length=610, Percent_Identity=23.4426229508197, Blast_Score=156, Evalue=3e-39, Organism=Escherichia coli, GI221142682, Length=459, Percent_Identity=24.1830065359477, Blast_Score=80, Evalue=3e-16, Organism=Escherichia coli, GI1788107, Length=559, Percent_Identity=21.824686940966, Blast_Score=74, Evalue=3e-14, Organism=Caenorhabditis elegans, GI32563687, Length=498, Percent_Identity=22.289156626506, Blast_Score=89, Evalue=5e-18, Organism=Caenorhabditis elegans, GI17550940, Length=441, Percent_Identity=24.9433106575964, Blast_Score=83, Evalue=5e-16, Organism=Caenorhabditis elegans, GI17556356, Length=424, Percent_Identity=23.8207547169811, Blast_Score=77, Evalue=3e-14, Organism=Caenorhabditis elegans, GI17558820, Length=488, Percent_Identity=22.3360655737705, Blast_Score=76, Evalue=5e-14, Organism=Caenorhabditis elegans, GI17560308, Length=382, Percent_Identity=24.869109947644, Blast_Score=69, Evalue=8e-12, Organism=Saccharomyces cerevisiae, GI6319591, Length=609, Percent_Identity=23.4811165845649, Blast_Score=115, Evalue=2e-26, Organism=Saccharomyces cerevisiae, GI6319699, Length=371, Percent_Identity=24.2587601078167, Blast_Score=75, Evalue=2e-14, Organism=Drosophila melanogaster, GI24648676, Length=575, Percent_Identity=22.6086956521739, Blast_Score=120, Evalue=4e-27,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR010071 - InterPro: IPR009081 - InterPro: IPR020845 - InterPro: IPR000873 - InterPro: IPR001242 - InterPro: IPR013624 - InterPro: IPR010060 - InterPro: IPR006163 - InterPro: IPR006162 [H]
Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF08415 NRPS; PF00550 PP-binding [H]
EC number: =5.1.1.3 [H]
Molecular weight: Translated: 70263; Mature: 70132
Theoretical pI: Translated: 8.27; Mature: 8.27
Prosite motif: PS50075 ACP_DOMAIN ; PS00455 AMP_BINDING
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.8 %Cys (Translated Protein) 2.0 %Met (Translated Protein) 3.8 %Cys+Met (Translated Protein) 1.8 %Cys (Mature Protein) 1.8 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSYNYFEKRQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT CCCCHHHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE YGHFMKRINALSNFLLNQKEFERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEEEEHHHHHHHHCCCEEEECCCCHHH INRIISLVERSKSKVVITNREYVNILKEKISESVNFIIIEDLDFSSLDSKENVIHIKADD HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECC PAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP CEEEEEECCCCCCCCCEEECHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEHH YLLNEVPLVCIERRKQYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCEEEEEHHHHHHHHHHHHHHH KFLSKYLPFYMVPLCHINIKDVPYTTNGKVHYKGLQKIFENKKGKVYLKSNLLYSKLELK HHHHHHCHHHEEHEEEEEEECCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCCHHHHHHH EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH KKLLNLILI HHHHHHHCC >Mature Secondary Structure SYNYFEKRQREDSILVGQDILSFRYNNSRIDTTIISCLRKIVNLSKEKVAIIDNETKIT CCCHHHHHCCCCCEEECCHHHHEEECCCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEE YGHFMKRINALSNFLLNQKEFERTSPITLILNGNYLRYVCVYGVLASGGYYLPIEPEVNN HHHHHHHHHHHHHHHCCHHHHCCCCCEEEEECCCEEEEHHHHHHHHCCCEEEECCCCHHH INRIISLVERSKSKVVITNREYVNILKEKISESVNFIIIEDLDFSSLDSKENVIHIKADD HHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEECC PAYMIYTSGSTGVPKGVIIPHRAVVNLVNALYVNFRLTKKDRGVAFTSFSFDSCGCDIYP CEEEEEECCCCCCCCCEEECHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCCCCEEHH YLLNEVPLVCIERRKQYVYDIEKLNKFCIDNMISIMFLPTVLAEKFITVDNNVLRVLYFG HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEEEEEEC GEKFCKNKVTNYELFNSYGLTETGILNTVYKIKGDEREIPLGDPIINSGILILDDQDKII CHHHHHCCCCCEEEECCCCCCCCCHHHEEEEECCCCCCCCCCCCCCCCCEEEECCCCCCC PKGKLGEIAIFGSGIAIEYDNNTELTNKKFIKLKEMGNLRCCKTGDLGYINENDNLYFKG CCCCCCEEEEEECCEEEEECCCCCCCCCCEEEEECCCCEEEEECCCCEEEECCCCEEEEC RKDKQVKISGYRIELDEIRFYLQKYACIELSIPMVIKKGKLSLLVNFYVAKEVLDEELIN CCCCEEEEEEEEEEHHHHHHHHHHHEEEEEECCEEEECCCEEEEEHHHHHHHHHHHHHHH KFLSKYLPFYMVPLCHINIKDVPYTTNGKVHYKGLQKIFENKKGKVYLKSNLLYSKLELK HHHHHHCHHHEEHEEEEEEECCCCCCCCEEEHHHHHHHHCCCCCEEEEECCCCHHHHHHH EFLIDTYANILKMDKDEIMEDGSFFEMGGDSLKAMKLQWYIQDELGVEVDISKIYEEFSI HHHHHHHHHHHHCCHHHHHCCCCEEECCCCCEEEEEEEEEEECCCCCEEEHHHHHHHHHH KKLLNLILI HHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 9427658 [H]