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The map label for this gene is rnfC [H]

Identifier: 226949820

GI number: 226949820

Start: 2772988

End: 2774106

Strand: Reverse

Name: rnfC [H]

Synonym: CLM_2761

Alternate gene names: 226949820

Gene position: 2774106-2772988 (Counterclockwise)

Preceding gene: 226949821

Following gene: 226949819

Centisome position: 66.76

GC content: 34.94

Gene sequence:

>1119_bases
ATGGAAAAAATGTTTAGTTTTCCTCTAAGGATGCACGTAGGTGCACCAGATGCTCCCTGTGTAAAAGAGGGACAAAAGGT
TAAAAGAGGAGAATGTATAGCAGAACCAAACGGACTTGGTGCTAAAATTCATACTAGTGTGTCCGGTATAGTAGAAAAAG
TTACAGATAAAGAAATCATCATAAAAGCAGATGAAGCTCAAACAACAGAATTCGTTAAGATAAAAAAATGTGATAATTTA
GTAGATACTGTATTTGAAGCAGGAATAGTTGGAGCTGGCGGAGCAGGTTTTCCAACTCACATAAAACTAAAGGCAGATAA
CAAAGATGGCTATATTATAGCAAACTGTGTAGAATGTGAACCAGCACTTCATCATAACATAAAAGTTATAGAAGAAACAC
CTGAATTAATAATAAATGGTGTTAGATATGCAATGAAAGCTACTAACTCAACAAAAGGATATATAGCTATAAAAGCTAAA
CATCCTGAAGCTATAGCTTCATTAGAAAAAGCTTTAAAGGGTGCTACTGATGTAGAAGTTAAACCTCTTGCTGACTTATA
TCCAATGGGTGAAGAAAGAGCTATAATCAATGCAATATTTGATAAGTGGTTAGATGTAACAGAGCTTCCTATAGAAGCAA
AATGCATTGTAATGAATGCTGAAACTTTAGCAAATATTACAAGAGCCGTAGAAGAAGGAAAGCCAGTTATAGATAAAGAT
ATAACAGTAATAGGAAAGTTAAAATCAGGCAACAAGCCAAATATATTCTTACAAGTACCAGTGGGAACACCAGTAAAAGC
TTTAATAGAAAAATCTGGTGGAATAGATGGTGAATATGGTGAACTAGTAATAGGTGGACCATACACTGGAAAAGCTGGAG
ACATAGAAAAGGATACAGTTACTAAAATATCTGGTGGAGCAATAGTTACAATACCACTACCAGAGTATAATGGACCATTA
GGACTACTAGTATGTGCCTGTGGAGCTAATGAAGAAAGATTAAAAGATGTAGCAACTAAAATGAATGCAAAAGTTGCAGG
AATAGTTGACTGCAAAAACATAGAATACCCAAAGGGTAAAGGAAATGGACCAGGAAAATGTAAAACACCTGGTGAATGA

Upstream 100 bases:

>100_bases
TATTTTGTGTTTTTTTTAACTTTTTTTATTTAAATTATAGATTTGGCATAAACATTGCTATATAATATAAGTGTGTGTAG
ATAGATCAAGGAGGGATACC

Downstream 100 bases:

>100_bases
CCAGGCCAAGCGGCTGCAGTTATGCAGCTTAAAAAAGATGGAGCAGAAAGAATATTAATATCAAACTGTAGTGATTGTTC
AAATACAGTTATGCAAATAG

Product: putative electron transport protein

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MEKMFSFPLRMHVGAPDAPCVKEGQKVKRGECIAEPNGLGAKIHTSVSGIVEKVTDKEIIIKADEAQTTEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNIKVIEETPELIINGVRYAMKATNSTKGYIAIKAK
HPEAIASLEKALKGATDVEVKPLADLYPMGEERAIINAIFDKWLDVTELPIEAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKALIEKSGGIDGEYGELVIGGPYTGKAGDIEKDTVTKISGGAIVTIPLPEYNGPL
GLLVCACGANEERLKDVATKMNAKVAGIVDCKNIEYPKGKGNGPGKCKTPGE

Sequences:

>Translated_372_residues
MEKMFSFPLRMHVGAPDAPCVKEGQKVKRGECIAEPNGLGAKIHTSVSGIVEKVTDKEIIIKADEAQTTEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNIKVIEETPELIINGVRYAMKATNSTKGYIAIKAK
HPEAIASLEKALKGATDVEVKPLADLYPMGEERAIINAIFDKWLDVTELPIEAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKALIEKSGGIDGEYGELVIGGPYTGKAGDIEKDTVTKISGGAIVTIPLPEYNGPL
GLLVCACGANEERLKDVATKMNAKVAGIVDCKNIEYPKGKGNGPGKCKTPGE
>Mature_372_residues
MEKMFSFPLRMHVGAPDAPCVKEGQKVKRGECIAEPNGLGAKIHTSVSGIVEKVTDKEIIIKADEAQTTEFVKIKKCDNL
VDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECEPALHHNIKVIEETPELIINGVRYAMKATNSTKGYIAIKAK
HPEAIASLEKALKGATDVEVKPLADLYPMGEERAIINAIFDKWLDVTELPIEAKCIVMNAETLANITRAVEEGKPVIDKD
ITVIGKLKSGNKPNIFLQVPVGTPVKALIEKSGGIDGEYGELVIGGPYTGKAGDIEKDTVTKISGGAIVTIPLPEYNGPL
GLLVCACGANEERLKDVATKMNAKVAGIVDCKNIEYPKGKGNGPGKCKTPGE

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=310, Percent_Identity=24.5161290322581, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788620, Length=236, Percent_Identity=26.6949152542373, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 39609; Mature: 39609

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKMFSFPLRMHVGAPDAPCVKEGQKVKRGECIAEPNGLGAKIHTSVSGIVEKVTDKEII
CCCCCCCCEEEECCCCCCCHHHCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCEEE
IKADEAQTTEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EEECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECC
PALHHNIKVIEETPELIINGVRYAMKATNSTKGYIAIKAKHPEAIASLEKALKGATDVEV
HHHHCCEEEEECCHHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEE
KPLADLYPMGEERAIINAIFDKWLDVTELPIEAKCIVMNAETLANITRAVEEGKPVIDKD
CCCHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNIFLQVPVGTPVKALIEKSGGIDGEYGELVIGGPYTGKAGDIEKDTV
EEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHH
TKISGGAIVTIPLPEYNGPLGLLVCACGANEERLKDVATKMNAKVAGIVDCKNIEYPKGK
EEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC
>Mature Secondary Structure
MEKMFSFPLRMHVGAPDAPCVKEGQKVKRGECIAEPNGLGAKIHTSVSGIVEKVTDKEII
CCCCCCCCEEEECCCCCCCHHHCCCCCCCCCEEECCCCCCEEEEHHHHHHHHHHCCCEEE
IKADEAQTTEFVKIKKCDNLVDTVFEAGIVGAGGAGFPTHIKLKADNKDGYIIANCVECE
EEECCCCCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEECC
PALHHNIKVIEETPELIINGVRYAMKATNSTKGYIAIKAKHPEAIASLEKALKGATDVEV
HHHHCCEEEEECCHHHHHHHHHHEEECCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEE
KPLADLYPMGEERAIINAIFDKWLDVTELPIEAKCIVMNAETLANITRAVEEGKPVIDKD
CCCHHHCCCCCCHHHHHHHHHHHCCHHCCCCCEEEEEECHHHHHHHHHHHHCCCCCCCCC
ITVIGKLKSGNKPNIFLQVPVGTPVKALIEKSGGIDGEYGELVIGGPYTGKAGDIEKDTV
EEEEEEECCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCCEEECCCCCCCCCCCCCCHH
TKISGGAIVTIPLPEYNGPLGLLVCACGANEERLKDVATKMNAKVAGIVDCKNIEYPKGK
EEECCCEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCC
GNGPGKCKTPGE
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]