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The map label for this gene is ackA [H]

Identifier: 226949813

GI number: 226949813

Start: 2765705

End: 2766898

Strand: Reverse

Name: ackA [H]

Synonym: CLM_2753

Alternate gene names: 226949813

Gene position: 2766898-2765705 (Counterclockwise)

Preceding gene: 226949814

Following gene: 226949812

Centisome position: 66.59

GC content: 33.67

Gene sequence:

>1194_bases
ATGAAAATATTAGTAGTTAACTGCGGAAGTTCGTCATTAAAGTATCAATTAATAGATATGACTTCTGAAGAGGCTTTAGC
AAAGGGATTAGTAGAGAGAATAGGAATAGAAGGCTCTATATTGACTCAAAAGGTTAATGGAGAAAAATATATAATAGAAG
AACCTATGAAAGATCATAAGAAAGCTATAGAGTTAGTTTTAAAAGCATTAGTAGACAAAGAGCATGGTGTAATATCTGAT
ATGTCAGAAATAGCAGCAGTAGGACATAGAGTAGTTCACGGTGGAGAAAAATATGCTAGCTCAGTACTTATAGATGATGA
GGTTATGAAGGCTTTAGAAGATTGTGTTAAATTAGCTCCACTTCATAATCCACCAAACATAATAGGAATAAATGCTTGTA
GAGAACTTATGCCTAAAACTCCAATGGTAGCAGTATTTGATACAGCATTCCATCAAACATTACCAGACTATGCATATATG
TATCCATTACCATATGAATTATATGAACAAAATGGTATAAGAAAATATGGTTTCCATGGAACATCACATAGATATGTATC
CAGTGTAGCGTCAGAGATGATGGGAAAAGATCTTAAAGATCTTAAAGTAATAACTTGTCACTTAGGAAATGGAGCAAGTT
TATGTGCTGTAAAAGAAGGAAAGTCAGTAGAAACCTCTATGGGATTCACTCCACTTGCAGGATTGGCTATGGGAACTAGA
TGCGGAGATATAGATCCAGCAATACTTCTTTTCATGGAAAGAGAATTAAAGATGTCACCAGATGAAGTAGATACCGTTAT
AAATAAAAAATCAGGTGTATTAGGAATATCAGGAGTAAGTAGTGACTTCAGAGACATAGAAGGTGCTGCAGAAGAAGGAA
ATAAAAGAGCTAAACTAGCTTTAGATGTATATCATTACACAGTAAGACAGACAATCGGTGCTTACACAGCAGTATTAAAT
GGTGTAGATGCTATAGTATTTACAGCAGGACTAGGAGAAAACTCAGCAGCAAGCAGAGAAGAAATTTTAAATGGATTAGA
ATACTTAGGTATTAAGATAGATGCCGAAAAGAATAAGCAAAGAGGAAAACAAATAGAAATAAGTACTGAAGATTCAAAGG
TAAAGGTATTCGTTATACCAACTGATGAAGAATTAATGATAGCAAGAGATACTAAAGAAATAACAGCTAAATAA

Upstream 100 bases:

>100_bases
TTTTAGATGATAAATTAATGTATTAATATCACGAATAAAAAAATAGATTTGCTTATAATAAAATTTTATATAAAACATAT
AGATGAAGGGTGGAAAATTA

Downstream 100 bases:

>100_bases
TATGTAATATAAGTTATAAATATAGGTATAATAACTTTGATTTACTTATAAAATACTTGACTTTTTAATATGCCCTTTAT
ATAATAAATTGTGGTTGCTA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITAK

Sequences:

>Translated_397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITAK
>Mature_397_residues
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHKKAIELVLKALVDKEHGVISD
MSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAPLHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYM
YPLPYELYEQNGIRKYGFHGTSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLALDVYHYTVRQTIGAYTAVLN
GVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQRGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITAK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=404, Percent_Identity=45.5445544554455, Blast_Score=345, Evalue=4e-96,
Organism=Escherichia coli, GI145693184, Length=392, Percent_Identity=43.3673469387755, Blast_Score=323, Evalue=9e-90,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 43404; Mature: 43404

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITAK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCHHHCCC
>Mature Secondary Structure
MKILVVNCGSSSLKYQLIDMTSEEALAKGLVERIGIEGSILTQKVNGEKYIIEEPMKDHK
CEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCHHHHH
KAIELVLKALVDKEHGVISDMSEIAAVGHRVVHGGEKYASSVLIDDEVMKALEDCVKLAP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHEEECHHHHHHHHHHHHHCC
LHNPPNIIGINACRELMPKTPMVAVFDTAFHQTLPDYAYMYPLPYELYEQNGIRKYGFHG
CCCCCCEEEHHHHHHHCCCCCEEEEEHHHHHHHCCCCEEECCCCHHHHHCCCCEEECCCC
TSHRYVSSVASEMMGKDLKDLKVITCHLGNGASLCAVKEGKSVETSMGFTPLAGLAMGTR
CCHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHCCC
CGDIDPAILLFMERELKMSPDEVDTVINKKSGVLGISGVSSDFRDIEGAAEEGNKRAKLA
CCCCCHHHHHEEHHHCCCCHHHHHHHHCCCCCEEEECCCCCHHHHHCCHHHCCCCEEEEE
LDVYHYTVRQTIGAYTAVLNGVDAIVFTAGLGENSAASREEILNGLEYLGIKIDAEKNKQ
EHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHEEEEEECCCCCC
RGKQIEISTEDSKVKVFVIPTDEELMIARDTKEITAK
CCCEEEEECCCCEEEEEEEECCCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA