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The map label for this gene is topA [H]

Identifier: 226949790

GI number: 226949790

Start: 2743767

End: 2745857

Strand: Reverse

Name: topA [H]

Synonym: CLM_2730

Alternate gene names: 226949790

Gene position: 2745857-2743767 (Counterclockwise)

Preceding gene: 226949791

Following gene: 226949789

Centisome position: 66.08

GC content: 30.42

Gene sequence:

>2091_bases
ATGGGACAAAATTTAGTAATAGTAGAATCACCAGCAAAAGCTAAAACCATAAAGAAGTATTTAGGAAAAAACTATGTAGT
TGAGGCCTCAATGGGACATGTAAGAGATTTACCTAAAAGTCAATTAGGTGTTGATATAGAAAATAATTATGATCCTAAGT
ATATAACAATAAGAGGAAAAGGCGAACTTTTAGATAAGCTTAGAAAAGAAGCTAAAAAAAGCTCTAAAGTATATTTAGCA
ACGGACCCGGATAGAGAGGGAGAAGCTATTTCTTGGCATTTAGCTCATGTTTTAAAAATAGATGAGAACGAAGAATGCAG
AATAGAATTTAACGAGATAACTAAAACTTCTATAAAGAAAGCAATCAAATCTCCGAGAAGAATAAATGAAAATGTAGTAG
ACGCGCAGCAAGCAAGAAGGGTTTTGGATAGATTAGTTGGATATAAAATAAGCCCTATATTGTGGAGCAAGGTTAAATGG
GGATTAAGTGCAGGTAGAGTTCAATCTGTGGCTTTAAGAATGATATGCGATAGAGAAAGAGAAATTGAAGAATTTATACC
AAAAGAATATTGGACTATAGAATGTGAGGTATATAAAGAAAAAAATAAAAAACCTTTCATAATAAAATTAACTACTAAAT
CAGGGAAAAAAATAGAGATAAATAATGAAGAGGAAGCTATTGAAGCAATACAAGACTTAAAAAAAGGTGAATTTATAGTA
AAAAAAATAAAGAAGACAAAGAAAAATAAAAATCCTTTAGCACCTTTTACTACGAGTACTCTTCAACAGGATTCTTATAA
AAAATTAAATTTTTCTACTAAAAAGACTATGTCTATAGCTCAACAACTATATGAAGGCATCGATATCAAAGGTTTTGGTG
CAGTAGGTCTTATAACCTACATGAGAACAGATTCTATAAGAATATCAGAAGAAGCTCAAAAATCAACCCTAGAGCATATC
AAAAGTAATTTTGGGGAAGAATATATACCAAAAACTCCAAGAGTTTTTAAAGGAAAGAAAAATATACAGGATGCCCATGA
AGCTATAAGACCAACCAATATTGAAATAACTCCTACACTTGCTAAGGAAAGTTTAACGCCTCAACAATATAAATTATACG
AATTAATATGGAAAAGATTTGTAGCAAGCCAAATGGCTTCTTGTATCTTAGATTCAACATCTATAAGTATAGAAAATGGA
AAATATGGATTAAGAGCTAGTGGATCCTTAATAGCTTTTGATGGTTTTATGAAGGTGTATGAGTATTCTACAGAGGAAGA
AAAAGATAGTATAAAAATACCTGAGTTGGAAGAGAATGAGGTTTTAAGAGAGAAATCTATAAACAAAAGCCAACATTTTA
CTCAACCACCAGCTAGGTATTCTGAAGCTTCTTTAGTAAAAACTTTAGAAGAAAATGGTATAGGAAGGCCAAGTACTTAT
GCTCCTATTATATCTACTATATTAGATAGAAAATATGTAGAAAGAGAGAAAAAAACATTAAAACCTACAGAACTAGGAGA
TATAGTAAATAATATACTAAGTGAATATTTTAAGCAAATAGTAGATCTAGAATTTACAGCGGATATGGAAAATAAGCTAG
ATTATATAGAAGAAGGAAAAAAATCTTGGAGAGAGGTAGTAAATGATTTCTTTCAGCCATTGATAGAATCCATAGAAATA
GCAGAAAAAGAAATAGCAAAAATAACTATTGAAGATAAAGTAACAGATGTGGTATGTGAAAAATGTGGAAGAAATATGGT
TATAAAACATGGAAGATATGGAGACTTTTTAGCATGTCCGGGATATCCAGATTGTAAAAATACCAAACCTATAGTAGAAG
AAATAGATGTTAAGTGTCCTAAATGTGGAGATGGAAAAGTCTTAATAAAGAAAAGTAGAAAAGGCAGAAAGTTTTATGGA
TGCAGTAACTATCCAGACTGTGATTTTGTAAGTTGGTCAGAACCTGTGAAAGAAAAGTGTGAAGAATGTGGAAGTTACAT
GGTAAAAAAACAAAGTAAAGCTAAAGGTGAATACTATGAATGTGCTAATTCAGAATGTAAGCATAGAAAATATTTAAATA
ATAAAGAGTAA

Upstream 100 bases:

>100_bases
TAGTAGCACCATAAAGAGGTGTAATTCTAAATTAGTATATCAGGTAAGGGGGTGTGACTCTGGTTATCAGTTTTACCAGA
GAAATAGAATGGGACAAAAA

Downstream 100 bases:

>100_bases
TGTATCAATAATTAATTTATATAAATAATTATTATTTAATATATTTTAAATGTTGAAATAAACAAACCCTTATGTTATTA
TAGATAAGGACAGGCAACAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSKVYLA
TDPDREGEAISWHLAHVLKIDENEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKVKW
GLSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGEFIV
KKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKSTLEHI
KSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENG
KYGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEENEVLREKSINKSQHFTQPPARYSEASLVKTLEENGIGRPSTY
APIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIESIEI
AEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCGDGKVLIKKSRKGRKFYG
CSNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE

Sequences:

>Translated_696_residues
MGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSKVYLA
TDPDREGEAISWHLAHVLKIDENEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKVKW
GLSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGEFIV
KKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKSTLEHI
KSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENG
KYGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEENEVLREKSINKSQHFTQPPARYSEASLVKTLEENGIGRPSTY
APIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIESIEI
AEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCGDGKVLIKKSRKGRKFYG
CSNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
>Mature_695_residues
GQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGKGELLDKLRKEAKKSSKVYLAT
DPDREGEAISWHLAHVLKIDENEECRIEFNEITKTSIKKAIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKVKWG
LSAGRVQSVALRMICDREREIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGEFIVK
KIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITYMRTDSIRISEEAQKSTLEHIK
SNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTLAKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENGK
YGLRASGSLIAFDGFMKVYEYSTEEEKDSIKIPELEENEVLREKSINKSQHFTQPPARYSEASLVKTLEENGIGRPSTYA
PIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGKKSWREVVNDFFQPLIESIEIA
EKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACPGYPDCKNTKPIVEEIDVKCPKCGDGKVLIKKSRKGRKFYGC
SNYPDCDFVSWSEPVKEKCEECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=591, Percent_Identity=24.5346869712352, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI4507635, Length=551, Percent_Identity=23.049001814882, Blast_Score=114, Evalue=5e-25,
Organism=Escherichia coli, GI1787529, Length=765, Percent_Identity=39.6078431372549, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1788061, Length=643, Percent_Identity=23.4836702954899, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI48994931, Length=140, Percent_Identity=32.8571428571429, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17555378, Length=509, Percent_Identity=26.3261296660118, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563869, Length=498, Percent_Identity=23.0923694779116, Blast_Score=110, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=549, Percent_Identity=22.9508196721311, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585251, Length=636, Percent_Identity=25, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24640096, Length=650, Percent_Identity=22.3076923076923, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 79901; Mature: 79769

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGK
CCCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCCCCCCCCEEEEECC
GELLDKLRKEAKKSSKVYLATDPDREGEAISWHLAHVLKIDENEECRIEFNEITKTSIKK
HHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCEEEHHHHHHHHHHH
AIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKVKWGLSAGRVQSVALRMICDRER
HHCCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHH
EIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGEFIV
HHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCHHHH
KKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITY
HHHHHHHCCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MRTDSIRISEEAQKSTLEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTL
HCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEEEECH
AKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENGKYGLRASGSLIAFDGFMKVY
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEHHHHHHH
EYSTEEEKDSIKIPELEENEVLREKSINKSQHFTQPPARYSEASLVKTLEENGIGRPSTY
HCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
APIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHH
KSWREVVNDFFQPLIESIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEECCCCCCEEECC
GYPDCKNTKPIVEEIDVKCPKCGDGKVLIKKSRKGRKFYGCSNYPDCDFVSWSEPVKEKC
CCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHH
EECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
HHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCCCC
>Mature Secondary Structure 
GQNLVIVESPAKAKTIKKYLGKNYVVEASMGHVRDLPKSQLGVDIENNYDPKYITIRGK
CCCEEEEECCHHHHHHHHHHCCCEEEECCCCHHHCCCHHHCCCCCCCCCCCCEEEEECC
GELLDKLRKEAKKSSKVYLATDPDREGEAISWHLAHVLKIDENEECRIEFNEITKTSIKK
HHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEECCCCCCCEEEHHHHHHHHHHH
AIKSPRRINENVVDAQQARRVLDRLVGYKISPILWSKVKWGLSAGRVQSVALRMICDRER
HHCCCHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCHH
EIEEFIPKEYWTIECEVYKEKNKKPFIIKLTTKSGKKIEINNEEEAIEAIQDLKKGEFIV
HHHHHCCCCEEEEEEEEEECCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHCCHHHH
KKIKKTKKNKNPLAPFTTSTLQQDSYKKLNFSTKKTMSIAQQLYEGIDIKGFGAVGLITY
HHHHHHHCCCCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHH
MRTDSIRISEEAQKSTLEHIKSNFGEEYIPKTPRVFKGKKNIQDAHEAIRPTNIEITPTL
HCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCHHHHHHHHCCCCCEEEEECH
AKESLTPQQYKLYELIWKRFVASQMASCILDSTSISIENGKYGLRASGSLIAFDGFMKVY
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCEEEEHHHHHHH
EYSTEEEKDSIKIPELEENEVLREKSINKSQHFTQPPARYSEASLVKTLEENGIGRPSTY
HCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHH
APIISTILDRKYVEREKKTLKPTELGDIVNNILSEYFKQIVDLEFTADMENKLDYIEEGK
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHH
KSWREVVNDFFQPLIESIEIAEKEIAKITIEDKVTDVVCEKCGRNMVIKHGRYGDFLACP
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHCCCCEEEECCCCCCEEECC
GYPDCKNTKPIVEEIDVKCPKCGDGKVLIKKSRKGRKFYGCSNYPDCDFVSWSEPVKEKC
CCCCCCCCCCHHHHHCCCCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCCHHHHHHH
EECGSYMVKKQSKAKGEYYECANSECKHRKYLNNKE
HHHHHHHHHHHHHHCCCHHHHCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]