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The map label for this gene is purB [H]

Identifier: 226949748

GI number: 226949748

Start: 2698657

End: 2700087

Strand: Reverse

Name: purB [H]

Synonym: CLM_2688

Alternate gene names: 226949748

Gene position: 2700087-2698657 (Counterclockwise)

Preceding gene: 226949750

Following gene: 226949744

Centisome position: 64.98

GC content: 31.52

Gene sequence:

>1431_bases
ATGAGAGATATTTATAATAATCCCTTAAACAAAAGATATTCATCTAAAGAAATGAGCTATTTATTTTCAGAAGAAATGAA
ATTTAAAACTTGGAGAAAATTATGGGTGGCCCTAGCAGAAAGCGAAAAGGAATTAGGTCTTAACATTACAGAAGAGCAAA
TAAATGAATTAAAAAAGTATATAGATGATATAAATTATGAAGTAGCAGAAGAGAGAGAAAAGGAAGTTCGACATGATGTT
ATGAGTCATGTATATGCTTATGGCGTTCAATGCCCTAAAGCAAAGGGTATAATACATTTAGGAGCTACTAGTTGTTATGT
AGGTGATAACACAGATTTAATTATAATGAAAGAAGCTATGATTTTAATAAAGAAGAAAATTATAAATGTTATAAATAATT
TGAAAAAGTTTGCCTTAGAGTATAAGCATGTACCAGCTTTAGGATTTACTCATTTTCAACCGGCTCAACTTACTACAGTA
GGTAAAAGAGCGACCCTTTGGATGCAAGATTTAGTATTAGACTTAGAACAATTAGAATTTGTTATAGATACCTTAAGATT
TAGAGGTGTTAAAGGAACTACAGGTACCCAAGCCAGCTTTATGGAATTGTTTGATGGAGATGAAAGTAAAGTAAAAGCTT
TAGATAAAAAGGTAGCAGAAAAGATGGGCTTCCCAAAGGAATTTATGGTAACAGGTCAAACCTATCCAAGAAAAGTTGAT
TCAACTATATTAAATACATTATCAGAAATAGCTCAAAGTGCATACAAATTTAGCAATGACTTAAGATTACTTCAAAGCAT
GAAGGAGATGGAAGAACCCTTCGAAAAAAATCAAATAGGTTCATCAGCTATGGCCTATAAGAGAAATCCAATGAGATCAG
AAAGAATGGGAGCTTTAGCAAGATACGTTATAGTAGATGCTTTAAATCCAGCTATAACCTCTTCTACGCAATGGTTTGAA
AGAACACTAGATGATTCTGCTAATAAGAGAATATCTATAGCAGAAGCTTTCCTAGCTTTAGATGGAGTGCTTAAACTATA
TATGAATATATCAGAGAATATGGTAGTATACGAAAAAGTTATAGAAAGTCATGTGAAAACAGAACTTCCATTTATGGCTA
CAGAAAATATAATGATGGAAGCTGTTAAAAAGGGTGGAGATAGACAAGAGCTTCACGAAGTTATAAGAACTCATTCTATG
GAAGCTGCAAGAAGAGTTAAGCAGGAAGGATTATCTAATGATCTAATAGAAAGAATAATAGAAGATAAATCCTTTGGATT
AACCAAAGAAGAAATATTAGCTTTAATAGATCCTAAAAAATTTACAGGAAGAGCAGAAGGTCAAGTAGTAGATTTTATAG
AAGAAGTAGTAAATCCAATATTAGAACAGAACAAAGCAATGCTAGGAGAAAAAGCAGAAATAAATGTATAA

Upstream 100 bases:

>100_bases
AATATACGAATATAAAATTGAAAAAATACTACAATATATGCTATAATATGAATAAATAAAATAAATATATACATATTGTA
CGTATAGAGGAGATGGAGTA

Downstream 100 bases:

>100_bases
TATTTAGTATAAAAATTTTAAAAGGAGCCATAATCTTGGCTCCTAAATTTTTATACTAATCTTTGAGCTAAAGCATTTTC
CAAGTTTAATTTTGAAATTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MRDIYNNPLNKRYSSKEMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPALGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFGLTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV

Sequences:

>Translated_476_residues
MRDIYNNPLNKRYSSKEMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPALGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFGLTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
>Mature_476_residues
MRDIYNNPLNKRYSSKEMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKYIDDINYEVAEEREKEVRHDV
MSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAMILIKKKIINVINNLKKFALEYKHVPALGFTHFQPAQLTTV
GKRATLWMQDLVLDLEQLEFVIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALARYVIVDALNPAITSSTQWFE
RTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKVIESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSM
EAARRVKQEGLSNDLIERIIEDKSFGLTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=474, Percent_Identity=58.0168776371308, Blast_Score=582, Evalue=1e-166,
Organism=Homo sapiens, GI183227688, Length=387, Percent_Identity=60.7235142118863, Blast_Score=512, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17508577, Length=465, Percent_Identity=41.9354838709677, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI32564234, Length=345, Percent_Identity=46.9565217391304, Blast_Score=319, Evalue=2e-87,
Organism=Saccharomyces cerevisiae, GI6323391, Length=478, Percent_Identity=54.3933054393305, Blast_Score=519, Evalue=1e-148,
Organism=Drosophila melanogaster, GI24647570, Length=457, Percent_Identity=56.0175054704595, Blast_Score=530, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 54521; Mature: 54521

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00163 FUMARATE_LYASES ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIYNNPLNKRYSSKEMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKY
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDDINYEVAEEREKEVRHDVMSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEHHHHH
ILIKKKIINVINNLKKFALEYKHVPALGFTHFQPAQLTTVGKRATLWMQDLVLDLEQLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
VIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHH
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
RYVIVDALNPAITSSTQWFERTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKV
HHHHHHHCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSMEAARRVKQEGLSNDLIERII
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDKSFGLTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
CCCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRDIYNNPLNKRYSSKEMSYLFSEEMKFKTWRKLWVALAESEKELGLNITEEQINELKKY
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHH
IDDINYEVAEEREKEVRHDVMSHVYAYGVQCPKAKGIIHLGATSCYVGDNTDLIIMKEAM
HHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEECCCCCEEEHHHHH
ILIKKKIINVINNLKKFALEYKHVPALGFTHFQPAQLTTVGKRATLWMQDLVLDLEQLEF
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
VIDTLRFRGVKGTTGTQASFMELFDGDESKVKALDKKVAEKMGFPKEFMVTGQTYPRKVD
HHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCHHHH
STILNTLSEIAQSAYKFSNDLRLLQSMKEMEEPFEKNQIGSSAMAYKRNPMRSERMGALA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHCCCCHHHHHHHHH
RYVIVDALNPAITSSTQWFERTLDDSANKRISIAEAFLALDGVLKLYMNISENMVVYEKV
HHHHHHHCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
IESHVKTELPFMATENIMMEAVKKGGDRQELHEVIRTHSMEAARRVKQEGLSNDLIERII
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
EDKSFGLTKEEILALIDPKKFTGRAEGQVVDFIEEVVNPILEQNKAMLGEKAEINV
CCCCCCCCHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]