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The map label for this gene is lexA [H]

Identifier: 226949036

GI number: 226949036

Start: 2010486

End: 2011091

Strand: Direct

Name: lexA [H]

Synonym: CLM_1952

Alternate gene names: 226949036

Gene position: 2010486-2011091 (Clockwise)

Preceding gene: 226949034

Following gene: 226949043

Centisome position: 48.38

GC content: 28.55

Gene sequence:

>606_bases
ATGAATAAAAGCCGAATTGACAAACAAAATGAAGTTTATAACTTTATAAAATTACAAATTAAAGAAAAAGGCTATCCTCC
TTCTGTAAGAGAAATTTGCAAGGCGGTAGGTTTAAGCTCAACTTCATCAGTGCATTTTCATCTTAAAAGATTAGAAAAAG
AAGGTTTAATAAAGAGAGATAGTAGCAAAACTAGAGCTATAGAAATTGTTGATCCTACCTCAAAAAAAGAAGTTATAAAT
GTGCCTATTGTAGGAACTATAACTGCAGGCAATCCTATTTTAGCTATAGAAAATATAGAAGATGTATTTCCATTACCTAT
TGATTATGTTAAAAACACAAAAGATTTATTTATGCTAAAGGTATCTGGAGAAAGTATGATAGAAGCTGGAATATTAGATG
GAGATTTAGCTATTATTGAAAAAACCGATTCTGCAAATAATGGAGATATTGTGGTTGCTCTAATAGACAACGAAGCTACT
TTAAAAAGATTTTTTAAAGAAAGCTCTTATATAAGACTTCAGCCTGAAAATAAAAGTATGAAACCTATAATTTTAGAGAA
TTGTAAAGTTCTTGGAAGATTAGTAGGAATATATAGAAAGTATTAA

Upstream 100 bases:

>100_bases
ATCATAAGTTATATTATTTTATAATTTATTTGAACATTAGTTTGCAGCTTTCCATTTTTATGGTATAATTTAATTATTAT
TTAATTAATGAGGTGTGATT

Downstream 100 bases:

>100_bases
TAACATTGTTATTTGGATAAATAAATGTGTATTAAAAATAATTTACTTTTAAAGAGGATTGTTAATAAAAATAATTCCTA
GGAGTATTAAGTGAATAACT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILDGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY

Sequences:

>Translated_201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILDGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY
>Mature_201_residues
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRDSSKTRAIEIVDPTSKKEVIN
VPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLKVSGESMIEAGILDGDLAIIEKTDSANNGDIVVALIDNEAT
LKRFFKESSYIRLQPENKSMKPIILENCKVLGRLVGIYRKY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family [H]

Homologues:

Organism=Escherichia coli, GI1790476, Length=201, Percent_Identity=36.318407960199, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787431, Length=135, Percent_Identity=28.1481481481481, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24 [H]

EC number: =3.4.21.88 [H]

Molecular weight: Translated: 22580; Mature: 22580

Theoretical pI: Translated: 9.28; Mature: 9.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRD
CCCCCCCCHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHEEHHHHCCCEECC
SSKTRAIEIVDPTSKKEVINVPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLK
CCCCEEEEEECCCCCCEEEECCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCEEEEEE
VSGESMIEAGILDGDLAIIEKTDSANNGDIVVALIDNEATLKRFFKESSYIRLQPENKSM
ECCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCC
KPIILENCKVLGRLVGIYRKY
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKSRIDKQNEVYNFIKLQIKEKGYPPSVREICKAVGLSSTSSVHFHLKRLEKEGLIKRD
CCCCCCCCHHHHEEEEEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHEEHHHHCCCEECC
SSKTRAIEIVDPTSKKEVINVPIVGTITAGNPILAIENIEDVFPLPIDYVKNTKDLFMLK
CCCCEEEEEECCCCCCEEEECCEEEEEECCCEEEEECCCCCCCCCCHHHHCCCCEEEEEE
VSGESMIEAGILDGDLAIIEKTDSANNGDIVVALIDNEATLKRFFKESSYIRLQPENKSM
ECCCCEEEECCCCCCEEEEEECCCCCCCCEEEEEECCCHHHHHHHCCCCEEEECCCCCCC
KPIILENCKVLGRLVGIYRKY
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA