BacMap

Definition	Clostridium botulinum A2 str. Kyoto chromosome, complete genome.
Accession	NC_012563
Length	4,155,278

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The map label for this gene is msbA [C]

Identifier: 226948615

GI number: 226948615

Start: 1567618

End: 1568298

Strand: Direct

Name: msbA [C]

Synonym: CLM_1510

Alternate gene names: 226948615

Gene position: 1567618-1568298 (Clockwise)

Preceding gene: 226948613

Following gene: 226948616

Centisome position: 37.73

GC content: 25.4

Gene sequence:

>681_bases
TTGAATGTAAATAAAAATTCTAATAAAATAAACAAAAAATTAAAAGTTATAAAAAACCATGAAGGTATAATAAGAGCTAC
AACAGACTTTACTATAATGCTTGCTATAGTAACTTATAGTTTAGTAGGATACAATTTGTATATGAAAAATATATTACATC
TTAGCTATATGATTTTAGGAATTGTTATTATCTCCTCATCCTTTGGGCCAGTAGTAGCTTTAAGTAATCTTTCAAATAAT
TTGCCCTATACCTTTGCCTCTACTGAAAGAGTATTTAGTATTTTAGATGAAGAGCCTATGGTAGAAGAAATAGACATGGG
TGAAAAAATATTAAATACTAATATAGTATATGATAATGTAAGCTTTTCCTATCAAGGAAGAAGAAATAAAATATTAGAAA
ATGTAAGATTATCTATAAATGAAAAAGAAAAGATAGCTATAGTAGGAGAAAGTGGTATAGGTAAAAGTACTTTTGTAAAG
CTTCTTATGAGATTTCGTGATGTTAATAAAGGGGATATTTTTATAGGAGAAAATTCTATTACAAATATAGAAACAAAAAA
TCTTAGAAAAAATCAAACACTAATAAGTCAAGAAACTTTTTTATTTAATGAAACTATAGAAGATAACATAAAAATGGGAA
ATATAAAAGCTAGTAGGGAACAGGTAATAGAAGCTGCTTAA

Upstream 100 bases:

>100_bases
TGTACGCCAATACAATTATACCACTTTATCTATGTTATGGTAATGTTTTATCAAAGATTTTCTAATTTTAATAATGTGCA
TGAGGATACAAATTTATAGA

Downstream 100 bases:

>100_bases
AAAGCATCTATACATGATTTTATAGAAAGCCTTCCTAAAGGCTATGAAACAAGGGTAGGAGAGCTTGGCGGGATTCTATC
TTCAGGAGAAAAACAAAGGA

Product: lipid A export ATP-binding/permease MsbA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MNVNKNSNKINKKLKVIKNHEGIIRATTDFTIMLAIVTYSLVGYNLYMKNILHLSYMILGIVIISSSFGPVVALSNLSNN
LPYTFASTERVFSILDEEPMVEEIDMGEKILNTNIVYDNVSFSYQGRRNKILENVRLSINEKEKIAIVGESGIGKSTFVK
LLMRFRDVNKGDIFIGENSITNIETKNLRKNQTLISQETFLFNETIEDNIKMGNIKASREQVIEAA

Sequences:

>Translated_226_residues
MNVNKNSNKINKKLKVIKNHEGIIRATTDFTIMLAIVTYSLVGYNLYMKNILHLSYMILGIVIISSSFGPVVALSNLSNN
LPYTFASTERVFSILDEEPMVEEIDMGEKILNTNIVYDNVSFSYQGRRNKILENVRLSINEKEKIAIVGESGIGKSTFVK
LLMRFRDVNKGDIFIGENSITNIETKNLRKNQTLISQETFLFNETIEDNIKMGNIKASREQVIEAA
>Mature_226_residues
MNVNKNSNKINKKLKVIKNHEGIIRATTDFTIMLAIVTYSLVGYNLYMKNILHLSYMILGIVIISSSFGPVVALSNLSNN
LPYTFASTERVFSILDEEPMVEEIDMGEKILNTNIVYDNVSFSYQGRRNKILENVRLSINEKEKIAIVGESGIGKSTFVK
LLMRFRDVNKGDIFIGENSITNIETKNLRKNQTLISQETFLFNETIEDNIKMGNIKASREQVIEAA

Specific function: Involved In Lipid A Export And Possibly Also In Glycerophospholipid Export And For Biogenesis Of The Outer Membrane. Transmembrane Domains (Tmd) Form A Pore In The Inner Membrane And The ATP-Binding Domain (Nbd) Is Responsible For Energy Generation. [C]

COG id: COG1132

COG function: function code V; ABC-type multidrug transport system, ATPase and permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI9955963, Length=173, Percent_Identity=32.3699421965318, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI9955968, Length=143, Percent_Identity=33.5664335664336, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI255708477, Length=142, Percent_Identity=35.9154929577465, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI9955966, Length=143, Percent_Identity=33.5664335664336, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI45243524, Length=143, Percent_Identity=33.5664335664336, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI42490749, Length=186, Percent_Identity=31.1827956989247, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI4505771, Length=192, Percent_Identity=28.6458333333333, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI9961250, Length=199, Percent_Identity=29.6482412060301, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI9961252, Length=181, Percent_Identity=28.7292817679558, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21536378, Length=200, Percent_Identity=34.5, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI42741659, Length=209, Percent_Identity=27.7511961722488, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI156105685, Length=118, Percent_Identity=35.5932203389831, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI148612844, Length=133, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI171184400, Length=145, Percent_Identity=31.7241379310345, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI73747917, Length=130, Percent_Identity=27.6923076923077, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787143, Length=161, Percent_Identity=34.1614906832298, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1786653, Length=170, Percent_Identity=30, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787112, Length=160, Percent_Identity=26.25, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1786654, Length=156, Percent_Identity=28.2051282051282, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI193202349, Length=176, Percent_Identity=33.5227272727273, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17541710, Length=140, Percent_Identity=37.8571428571429, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI25146777, Length=177, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71996809, Length=180, Percent_Identity=35, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI193209708, Length=122, Percent_Identity=38.5245901639344, Blast_Score=90, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI193211017, Length=175, Percent_Identity=34.2857142857143, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI212646699, Length=176, Percent_Identity=32.3863636363636, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193211015, Length=175, Percent_Identity=34.2857142857143, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI193208177, Length=227, Percent_Identity=28.6343612334802, Blast_Score=84, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17569145, Length=129, Percent_Identity=35.6589147286822, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567265, Length=143, Percent_Identity=33.5664335664336, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17565938, Length=156, Percent_Identity=32.6923076923077, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71984940, Length=142, Percent_Identity=33.0985915492958, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17533971, Length=154, Percent_Identity=29.2207792207792, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17569143, Length=166, Percent_Identity=31.3253012048193, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17567269, Length=142, Percent_Identity=31.6901408450704, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI115535101, Length=130, Percent_Identity=31.5384615384615, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533592, Length=138, Percent_Identity=36.231884057971, Blast_Score=77, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71992285, Length=146, Percent_Identity=33.5616438356164, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI71996550, Length=224, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558664, Length=203, Percent_Identity=31.0344827586207, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI115534520, Length=195, Percent_Identity=28.2051282051282, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17543740, Length=157, Percent_Identity=29.9363057324841, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71992290, Length=133, Percent_Identity=29.3233082706767, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323959, Length=227, Percent_Identity=30.8370044052863, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323217, Length=203, Percent_Identity=33.0049261083744, Blast_Score=91, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6322640, Length=108, Percent_Identity=39.8148148148148, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324985, Length=201, Percent_Identity=28.8557213930348, Blast_Score=74, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322980, Length=143, Percent_Identity=32.1678321678322, Blast_Score=70, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321720, Length=115, Percent_Identity=33.9130434782609, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24659289, Length=188, Percent_Identity=35.6382978723404, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI85726357, Length=182, Percent_Identity=28.021978021978, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357907, Length=144, Percent_Identity=37.5, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281363262, Length=117, Percent_Identity=40.1709401709402, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653245, Length=117, Percent_Identity=40.1709401709402, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17136662, Length=176, Percent_Identity=28.4090909090909, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24641565, Length=146, Percent_Identity=32.1917808219178, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI21357851, Length=115, Percent_Identity=39.1304347826087, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551501, Length=115, Percent_Identity=39.1304347826087, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI45551502, Length=115, Percent_Identity=39.1304347826087, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221474134, Length=97, Percent_Identity=34.020618556701, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017940
- InterPro:   IPR001140
- InterPro:   IPR011527
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00664 ABC_membrane; PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25608; Mature: 25608

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVNKNSNKINKKLKVIKNHEGIIRATTDFTIMLAIVTYSLVGYNLYMKNILHLSYMILG
CCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIISSSFGPVVALSNLSNNLPYTFASTERVFSILDEEPMVEEIDMGEKILNTNIVYDNV
HHHHCCCCCCEEEECCCCCCCCEEEECHHHHHHHHCCCCCHHHHCCCHHHHCCEEEEECC
SFSYQGRRNKILENVRLSINEKEKIAIVGESGIGKSTFVKLLMRFRDVNKGDIFIGENSI
EEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCC
TNIETKNLRKNQTLISQETFLFNETIEDNIKMGNIKASREQVIEAA
CCCCCCCCCCCCHHHHHHHHEECCHHHCCEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MNVNKNSNKINKKLKVIKNHEGIIRATTDFTIMLAIVTYSLVGYNLYMKNILHLSYMILG
CCCCCCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVIISSSFGPVVALSNLSNNLPYTFASTERVFSILDEEPMVEEIDMGEKILNTNIVYDNV
HHHHCCCCCCEEEECCCCCCCCEEEECHHHHHHHHCCCCCHHHHCCCHHHHCCEEEEECC
SFSYQGRRNKILENVRLSINEKEKIAIVGESGIGKSTFVKLLMRFRDVNKGDIFIGENSI
EEEECCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCC
TNIETKNLRKNQTLISQETFLFNETIEDNIKMGNIKASREQVIEAA
CCCCCCCCCCCCHHHHHHHHEECCHHHCCEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]