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The map label for this gene is rnjA [H]

Identifier: 226948561

GI number: 226948561

Start: 1504667

End: 1506331

Strand: Direct

Name: rnjA [H]

Synonym: CLM_1453

Alternate gene names: 226948561

Gene position: 1504667-1506331 (Clockwise)

Preceding gene: 226948560

Following gene: 226948562

Centisome position: 36.21

GC content: 32.01

Gene sequence:

>1665_bases
ATGAGCAATAAAGATAGTGTAAAGGTCATTCCTTTAGGTGGACTTGGGGAAATTGGTAAAAATATAACTGCTTTTGAATA
TGGAAATGAAATAGTAATCATAGATTGTGGTATATCTTTTCCAGATGAGGAGATGTATGGTGTAGATTTGGTTATTCCGG
ATATAACCTACCTTCTAAATAACAAAGATAAAGTAAAAGCCATTTTTCTAACTCATGGACATGAAGATCATATAGGATCT
TTACCTTATATTCTGCAACAATTAAATAGGACTGTTTATGGGACTGCATTAACCTTAGGTATAGTAGAGAATAAACTAAA
AGAACATAATATGCTTTCAGATTGTGAGCTTAATAAGGTGGAAGCAGGAGATATAGTAGAACTTAAAAATTTAAAGATAG
AATTTATAAGAAATACTCATAGTATAGCAGATTCTTGTTCCATAGCTATTCATACGCCGGTAGGAGTTATATTACATACT
GGCGATTTTAAAATAGACTATACACCTATTGATGGATTAGTAATGGATTTTCATCGTATTGCTGAATTAGGTAAAAAGGG
AGTATTACTTCTCATGGCTGATAGCACAAACGTACAGCGTAAGGGACACACAATTTCCGAGAAATCTATAGGAGAAACCT
TAACTAAAATTTTTTCAAATGCTAAAGGAAGAGTTATTGTAGCGACTTTTGCTTCAAACATACATAGGATGCAGCAAATA
ATAGATGCATCTATAGAGTATGGTAGAAAAGTAGCCTTCAGTGGGAGAAGTATGGAAAATATATCTAAGGTAGCCATGGA
CTTAGGATATCTTCATATACCAGAAACTTATTTAATAACTGTAGATGAAATGAAAAATTATCCTAACGAACAAATAACCA
TAATAACTACTGGAAGTCAAGGAGAACCTATGGCAGCTCTTGCAAGAATAGCTTATTCTAATCATAGAAAAATAGCTATA
GAGCCAAAGGATTTATTTATTATTTCTGCATCACCAATTCCAGGAAATGAAAAACTTATATCTAGAGTAATAAATGAGTT
ATTTAAAAAGGGTGCAGATGTAATATATGAAGCATTAGAAGAGGTACATGTATCTGGTCATGCTTATGAGGAAGAATTAA
AATTAATTCATACATTGGTGCATCCTAAATATTTTATGCCAGTACATGGAGAATACAGGCACTTAAAACGACATGTGGAT
TTAGCCATGGAGTTAGGCATGGAAAGAGAGAATATATTCTCTTTAGAAACAGGGCAAGTTTTAGAAATATCGCATGAAGA
AGCAAAAGTATCAGGGAAGGTCCGTACAGGATCTATATTTGTAGATGGTATAGGTGTAGGTGATGTAGGGAATATAGTGC
TCAGAGACAGAAGGTATTTAGCGCAGGACGGTATGTTAACCATAGTAGTAACTCTAGAAAAAGAATCCTACAGCGTAATA
GCAGGACCGGATGTAATAACAAGGGGATTCATATATGTAAAAGAGTCAGAGGATTTAATAAATGAAGTAAAAGAAATAGT
AAAAAAAGAACTAGAAAATTGTTTAGAAAATAAAATAATTGAATGGTATGTATTAAAATCAAATATTAAAAAATCTGTAG
AAAAATATTTATATGAAAAAACAAAGAGAAGGCCAATAGTTTTGCCTATAATTATGGAAATTTAA

Upstream 100 bases:

>100_bases
ATAATCATTAGTTCTTTAGTTAATTTTATTAATCTAAAGAGCAAAATAATATAATAAGTATAGCAAAAAATTTATAAATA
TTGAAAGAGGAGGGTATGAA

Downstream 100 bases:

>100_bases
AATTATAAAAAATAACAAAAAGCTAGAACTTTAATATGAAATTATGGTTCTAGCTTTTTGTTATATATTAATACACTAAA
ATGATGTAAATATAATAAAA

Product: RNA-metabolising metallo-beta-lactamase

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 554; Mature: 553

Protein sequence:

>554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRTVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
EPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDVITRGFIYVKESEDLINEVKEIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Sequences:

>Translated_554_residues
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGS
LPYILQQLNRTVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHT
GDFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAI
EPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVD
LAMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
AGPDVITRGFIYVKESEDLINEVKEIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI
>Mature_553_residues
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLNNKDKVKAIFLTHGHEDHIGSL
PYILQQLNRTVYGTALTLGIVENKLKEHNMLSDCELNKVEAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTG
DFKIDYTPIDGLVMDFHRIAELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQII
DASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQGEPMAALARIAYSNHRKIAIE
PKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALEEVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDL
AMELGMERENIFSLETGQVLEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVIA
GPDVITRGFIYVKESEDLINEVKEIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEKTKRRPIVLPIIMEI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 62197; Mature: 62066

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRTVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIEPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDVITRGFIYVKESEDLINEVKEIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC
>Mature Secondary Structure 
SNKDSVKVIPLGGLGEIGKNITAFEYGNEIVIIDCGISFPDEEMYGVDLVIPDITYLLN
CCCCCEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCCCCEEEEEECCCCEECC
NKDKVKAIFLTHGHEDHIGSLPYILQQLNRTVYGTALTLGIVENKLKEHNMLSDCELNKV
CCCCEEEEEEECCCCCCCCCHHHHHHHHCCHHHHHHEEHHHHHHHHHHCCCCCCCCCCCC
EAGDIVELKNLKIEFIRNTHSIADSCSIAIHTPVGVILHTGDFKIDYTPIDGLVMDFHRI
CCCCEEEEECCEEEEECCHHHHCCCCEEEEECCEEEEEECCCEEEEEECCCHHHHHHHHH
AELGKKGVLLLMADSTNVQRKGHTISEKSIGETLTKIFSNAKGRVIVATFASNIHRMQQI
HHCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHH
IDASIEYGRKVAFSGRSMENISKVAMDLGYLHIPETYLITVDEMKNYPNEQITIITTGSQ
HHHHHHHCCEEEECCCCHHHHHHHHHHCCEEECCCEEEEEEHHHHCCCCCEEEEEEECCC
GEPMAALARIAYSNHRKIAIEPKDLFIISASPIPGNEKLISRVINELFKKGADVIYEALE
CCHHHHHHHHHHCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
EVHVSGHAYEEELKLIHTLVHPKYFMPVHGEYRHLKRHVDLAMELGMERENIFSLETGQV
HHHCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCCHHHEEEECCCCE
LEISHEEAKVSGKVRTGSIFVDGIGVGDVGNIVLRDRRYLAQDGMLTIVVTLEKESYSVI
EEEECCHHHCCCEEEECEEEEEECCCCCCCCEEECCCHHHHCCCCEEEEEEEECCCEEEE
AGPDVITRGFIYVKESEDLINEVKEIVKKELENCLENKIIEWYVLKSNIKKSVEKYLYEK
ECCCHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHH
TKRRPIVLPIIMEI
HCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]