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The map label for this gene is iscS [H]

Identifier: 226948449

GI number: 226948449

Start: 1393386

End: 1394516

Strand: Direct

Name: iscS [H]

Synonym: CLM_1333

Alternate gene names: 226948449

Gene position: 1393386-1394516 (Clockwise)

Preceding gene: 226948448

Following gene: 226948450

Centisome position: 33.53

GC content: 31.83

Gene sequence:

>1131_bases
ATGAGAAATAAGATATACATGGATCATGCAGCCACTACTTATATAAAGAAAGAAGTAATGGACGCTATGATTCCCTACTT
AACAGAATATTATGCAAATCCTTCTTCTGTATATAATATGGCTAATAATTTAAGAATTGCTATAGATGAAGCAAAAGAAG
AAATTGCAGATTTTATAGGAGGAGAGTCAGAGGAAATATTTTTTACCTCTGGTGGTACAGAAGGAGATAATTGGGCTATA
AAAGGGATAGCTTATGGAAATGAAAACAAAGGAAAACACATAATAACGTCTTCTATAGAACATCCAGCAGTTTTAAATAG
TTGTAAATATTTAAGAGAAAAAGGTTTTGAAATAACATTTTTGACTGTAGATGGTTATGGGAAAATAGATTTAAAAGAAT
TGGAAGAAAGCATAAGGGAAGATACTATATTAGTTTCTATTATGACTGCCAATAATGAGATAGGCACTATACAAGATATA
AGATCCATAGGAGCAATCTGTAAGGCACATAAAGTTTTATTCCATACAGATGCAGTTCAAGCTTTAGGTCAAACATCTAT
AAATGTGAAAGAAATGAACATAGACTTAATGACTATAACTGCACACAAAATATATGGTCCTAAAGGGATTGGAGCATTAT
ATATAAAAAAAGGCACAAAAATAGATAATCTTTTCCATGGTGGTAGCCAAGAAAGAGGAAAAAGAGCAGGAACAGAAAAT
CCAGCTGCTATAGTTGGCTTTAAAAAGGCGGTTTCATTGCTTAAAGAAAAGGGCCAAGAGGAAAATAAGAGAATAGAAAA
GCTTAGAGATAAATTTATAAAGGGACTTTTACAAATAGAAGGAACTAAAATAAATGGTGCTCTAGGTATAGAAAGACTAA
AAGGAAACATAAATGTTAGTTTTAAAAATGCAGATGGGGAGCTATTACTTATGCTTTTAGATAGTGAAGGTATATGTGCT
TCAGCGGGAAGTGCTTGTTCAGCAGGAGCAGTAGATGCATCTCATGTGCTTTTAGCCTTAGGATTAGATAAGGAATTTTT
AAAAGGAACTATAAGATTTACTCTAGGGGCTAAAAATACAGAAGAAGAAATAGATTTTGTATTAGAAAAATTAAAGGAAA
TAGTAAAGTGA

Upstream 100 bases:

>100_bases
TCTATTTGATTAAATTTAAATATTTTTAATATAAAATTTTAAAGATATTAATATAAAATCCATAGTTACAAATAATAAAA
TAAAAAACAGTGGTGATTAT

Downstream 100 bases:

>100_bases
TCTTTATAGAATATGGAAGTATTAGTCAGCATAATAATAGGACAAAAATATAAGATGGGGGTGGTATAAAAATGAATTAT
AAATATAATAATCTTATTAA

Product: class V aminotransferase

Products: NA

Alternate protein names: NifS protein homolog [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRNKIYMDHAATTYIKKEVMDAMIPYLTEYYANPSSVYNMANNLRIAIDEAKEEIADFIGGESEEIFFTSGGTEGDNWAI
KGIAYGNENKGKHIITSSIEHPAVLNSCKYLREKGFEITFLTVDGYGKIDLKELEESIREDTILVSIMTANNEIGTIQDI
RSIGAICKAHKVLFHTDAVQALGQTSINVKEMNIDLMTITAHKIYGPKGIGALYIKKGTKIDNLFHGGSQERGKRAGTEN
PAAIVGFKKAVSLLKEKGQEENKRIEKLRDKFIKGLLQIEGTKINGALGIERLKGNINVSFKNADGELLLMLLDSEGICA
SAGSACSAGAVDASHVLLALGLDKEFLKGTIRFTLGAKNTEEEIDFVLEKLKEIVK

Sequences:

>Translated_376_residues
MRNKIYMDHAATTYIKKEVMDAMIPYLTEYYANPSSVYNMANNLRIAIDEAKEEIADFIGGESEEIFFTSGGTEGDNWAI
KGIAYGNENKGKHIITSSIEHPAVLNSCKYLREKGFEITFLTVDGYGKIDLKELEESIREDTILVSIMTANNEIGTIQDI
RSIGAICKAHKVLFHTDAVQALGQTSINVKEMNIDLMTITAHKIYGPKGIGALYIKKGTKIDNLFHGGSQERGKRAGTEN
PAAIVGFKKAVSLLKEKGQEENKRIEKLRDKFIKGLLQIEGTKINGALGIERLKGNINVSFKNADGELLLMLLDSEGICA
SAGSACSAGAVDASHVLLALGLDKEFLKGTIRFTLGAKNTEEEIDFVLEKLKEIVK
>Mature_376_residues
MRNKIYMDHAATTYIKKEVMDAMIPYLTEYYANPSSVYNMANNLRIAIDEAKEEIADFIGGESEEIFFTSGGTEGDNWAI
KGIAYGNENKGKHIITSSIEHPAVLNSCKYLREKGFEITFLTVDGYGKIDLKELEESIREDTILVSIMTANNEIGTIQDI
RSIGAICKAHKVLFHTDAVQALGQTSINVKEMNIDLMTITAHKIYGPKGIGALYIKKGTKIDNLFHGGSQERGKRAGTEN
PAAIVGFKKAVSLLKEKGQEENKRIEKLRDKFIKGLLQIEGTKINGALGIERLKGNINVSFKNADGELLLMLLDSEGICA
SAGSACSAGAVDASHVLLALGLDKEFLKGTIRFTLGAKNTEEEIDFVLEKLKEIVK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=378, Percent_Identity=43.6507936507937, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI156713448, Length=410, Percent_Identity=33.4146341463415, Blast_Score=211, Evalue=1e-54,
Organism=Escherichia coli, GI48994898, Length=381, Percent_Identity=44.3569553805774, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI1787970, Length=389, Percent_Identity=24.1645244215938, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789175, Length=388, Percent_Identity=23.1958762886598, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI25143064, Length=371, Percent_Identity=40.4312668463612, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17533177, Length=323, Percent_Identity=30.6501547987616, Blast_Score=158, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6319831, Length=376, Percent_Identity=45.2127659574468, Blast_Score=334, Evalue=2e-92,
Organism=Drosophila melanogaster, GI20129463, Length=370, Percent_Identity=40.8108108108108, Blast_Score=298, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41272; Mature: 41272

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNKIYMDHAATTYIKKEVMDAMIPYLTEYYANPSSVYNMANNLRIAIDEAKEEIADFIG
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEEHHHHHHHHHHHC
GESEEIFFTSGGTEGDNWAIKGIAYGNENKGKHIITSSIEHPAVLNSCKYLREKGFEITF
CCCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEE
LTVDGYGKIDLKELEESIREDTILVSIMTANNEIGTIQDIRSIGAICKAHKVLFHTDAVQ
EEECCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
ALGQTSINVKEMNIDLMTITAHKIYGPKGIGALYIKKGTKIDNLFHGGSQERGKRAGTEN
HHCCCCCEEEEEEEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHCCCCHHHHCCCCCCC
PAAIVGFKKAVSLLKEKGQEENKRIEKLRDKFIKGLLQIEGTKINGALGIERLKGNINVS
CCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHEEECCEEEEE
FKNADGELLLMLLDSEGICASAGSACSAGAVDASHVLLALGLDKEFLKGTIRFTLGAKNT
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHEEEEECCCHHHHCEEEEEEECCCCC
EEEIDFVLEKLKEIVK
HHHHHHHHHHHHHHHC
>Mature Secondary Structure
MRNKIYMDHAATTYIKKEVMDAMIPYLTEYYANPSSVYNMANNLRIAIDEAKEEIADFIG
CCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCEEEEEHHHHHHHHHHHC
GESEEIFFTSGGTEGDNWAIKGIAYGNENKGKHIITSSIEHPAVLNSCKYLREKGFEITF
CCCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEE
LTVDGYGKIDLKELEESIREDTILVSIMTANNEIGTIQDIRSIGAICKAHKVLFHTDAVQ
EEECCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEEHHHHH
ALGQTSINVKEMNIDLMTITAHKIYGPKGIGALYIKKGTKIDNLFHGGSQERGKRAGTEN
HHCCCCCEEEEEEEEEEEEEEEEECCCCCCCEEEEECCCCHHHHHCCCCHHHHCCCCCCC
PAAIVGFKKAVSLLKEKGQEENKRIEKLRDKFIKGLLQIEGTKINGALGIERLKGNINVS
CCEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHEEECCEEEEE
FKNADGELLLMLLDSEGICASAGSACSAGAVDASHVLLALGLDKEFLKGTIRFTLGAKNT
EECCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHEEEEECCCHHHHCEEEEEEECCCCC
EEEIDFVLEKLKEIVK
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10930739 [H]