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The map label for this gene is folD [H]

Identifier: 226948439

GI number: 226948439

Start: 1384576

End: 1385424

Strand: Direct

Name: folD [H]

Synonym: CLM_1323

Alternate gene names: 226948439

Gene position: 1384576-1385424 (Clockwise)

Preceding gene: 226948438

Following gene: 226948440

Centisome position: 33.32

GC content: 27.33

Gene sequence:

>849_bases
ATGACTAAAATATTATATGGAAATGAAGTAGCTTTAAAAATAAAGGAAGATTTAAATTTAAGAATAGACAAATTAAAAGA
AAAAAATATAATACCTAAGTTAGCAATTCTACGTATGGGAAATAAACCGGACGATATAGCCTATGAAAGAAGTATAATAA
AAAGCTGTGAAAAACTAAACATAGAAACTAAGGTAGAAGAATTAAATGAAGATATATTAGAAGAAGATTTTTTGAAGTTA
ATGGAAAGTTTAAATAACGAAAAGGAAATTCATGGTATATTAGTCTTTAGACCTTACCCTAAACATTTAAATGAAAATAC
AATAAACTCTTCTATAGCATTAAATAAAGATGTGGATTGCATGCATCCTTTAAATTTAGAAAGGATATTTGAAGGAGATT
TAAATCAGTTTGTGCCTTGTACTCCAGAGGCTGTAATAGAAATATTAAAATATTATGATATAGATTTAAAAGGAAAGAAT
ATAGTTATTATAAACAGAAGTATGGTAGTGGGCAAACCATTGAGTATGATGGTCTTATCTAATAATGCTACAGTTACTAT
ATGCCATTCAAAAACTATAGATTTGCCATCTATAACTAAAAAAGCAGATATAGTAGTGACAGCTATAGGAAAAGCTAAAT
TAATAAAAGAAGAATATTTTAATGAAGATTCTATAGTTATGGATGTAAGCATTAATGTAGATGAAAATGGAAAACTATGT
GGAGATGTGGATTTTGAAAATGTAAAAGAAAAAGTAGGAGCTATAACTCCAGTTCCAAAAGGAGTAGGAAGTGTTACAAC
TACCTTGTTATTAAAACACATAGTAGATGCAGCAGAGAGAAATAGTTAA

Upstream 100 bases:

>100_bases
ATTCGTTGAAAGAAGAAATCAACACCTTAGTTTCTAAATATTCAACTAAGGCAGATAAAGTTTATAGTTATGTAGAAAAT
TTAATAAGGGGAAATGAATA

Downstream 100 bases:

>100_bases
ATATAAAAATTAAAAAGTAAAGCCTGTATCCTATGTTAGTTTATATAGGATACAGGCTTATTTTTCTTATAATAAATAAT
ATATAAGATTTTATAGATTC

Product: methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MTKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MESLNNEKEIHGILVFRPYPKHLNENTINSSIALNKDVDCMHPLNLERIFEGDLNQFVPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINVDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS

Sequences:

>Translated_282_residues
MTKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKL
MESLNNEKEIHGILVFRPYPKHLNENTINSSIALNKDVDCMHPLNLERIFEGDLNQFVPCTPEAVIEILKYYDIDLKGKN
IVIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINVDENGKLC
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
>Mature_281_residues
TKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLNIETKVEELNEDILEEDFLKLM
ESLNNEKEIHGILVFRPYPKHLNENTINSSIALNKDVDCMHPLNLERIFEGDLNQFVPCTPEAVIEILKYYDIDLKGKNI
VIINRSMVVGKPLSMMVLSNNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINVDENGKLCG
DVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222136639, Length=293, Percent_Identity=36.8600682593857, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=36.8600682593857, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=34.5762711864407, Blast_Score=147, Evalue=9e-36,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=33.9350180505415, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI17568735, Length=291, Percent_Identity=32.9896907216495, Blast_Score=156, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=38.3275261324042, Blast_Score=208, Evalue=8e-55,
Organism=Saccharomyces cerevisiae, GI6321643, Length=269, Percent_Identity=37.9182156133829, Blast_Score=173, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6322933, Length=288, Percent_Identity=21.5277777777778, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI62472483, Length=288, Percent_Identity=34.375, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI45551871, Length=288, Percent_Identity=34.375, Blast_Score=180, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24645718, Length=288, Percent_Identity=34.375, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17137370, Length=288, Percent_Identity=34.375, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=32.3232323232323, Blast_Score=157, Evalue=6e-39,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=32.3232323232323, Blast_Score=157, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31688; Mature: 31557

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLN
CCEEEECCEEEEEEECCCCCEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMESLNNEKEIHGILVFRPYPKHLNENTINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCC
MHPLNLERIFEGDLNQFVPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCEEEEEEE
NNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINVDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKILYGNEVALKIKEDLNLRIDKLKEKNIIPKLAILRMGNKPDDIAYERSIIKSCEKLN
CEEEECCEEEEEEECCCCCEEEHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCC
IETKVEELNEDILEEDFLKLMESLNNEKEIHGILVFRPYPKHLNENTINSSIALNKDVDC
CHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEECCCCCC
MHPLNLERIFEGDLNQFVPCTPEAVIEILKYYDIDLKGKNIVIINRSMVVGKPLSMMVLS
CCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEECCCEEEEEECCEEECCCEEEEEEE
NNATVTICHSKTIDLPSITKKADIVVTAIGKAKLIKEEYFNEDSIVMDVSINVDENGKLC
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCCEEEEEEEEECCCCCEE
GDVDFENVKEKVGAITPVPKGVGSVTTTLLLKHIVDAAERNS
CCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA