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The map label for this gene is ynjE [H]

Identifier: 226948413

GI number: 226948413

Start: 1355362

End: 1356744

Strand: Direct

Name: ynjE [H]

Synonym: CLM_1297

Alternate gene names: 226948413

Gene position: 1355362-1356744 (Clockwise)

Preceding gene: 226948412

Following gene: 226948414

Centisome position: 32.62

GC content: 31.02

Gene sequence:

>1383_bases
GTGAAAAAGAACTTGAAAGGAATAGTGTTATTGCTATTCGGTATTATGATGTTTACATTAATAGGTTGTACCAGTAACCA
AAAAACAGAGGAGAAGAAAGAAAGTACTCCTTTAGGAAAGAAAATTAAAACAGATGAATTAAAGAAAAATATAACATCCA
AGGAATGGGTAGTAGTAGACACAAGATCCAATGATGCTTTTAATGGATGGAAATTAGATGGAGTAAAAAGAGGTGGACAT
ATAAAAGGTGCTACTGATTTTTCAGCAAATTGGCTAAAAGTAGAAGATACTGAAAAGAATAAAGAAAAAAGATTAGAAGA
GGCTTTAAAAAATAAAGGTATCACAAAGGATAAAAATGTAGTTTTATATGATGCTAATGGAAAAGATGCAGAAGAAGTAG
CAAAATATTTAAATAAAAAAGGAATAACTAAGTTATATAAATATGATGTTAAAGAATGGGCAGAAGATGATAAACTTGCA
ATGGAAAGTTATTCTAATTATCAAATGTTAGTACCAGCTAGTTGGGTTAATGATTTAATAAATAATAAAAAGCCAGAAAC
TTTTAAAGGAGATAAATATAAGGTTTTTGAAGTGAGCTGGGGAGATGAATCTAAAGCAGAAGATTACAAAAAAGGACATA
TTAAAGGTGCTGTACATATAAATACAGATGAAGTTGAAAAAGGACCAGTATGGAATCGTCTACCAGATAAGGATTTAGAA
AAGTTTGCTAAAAATAATGGTATTACAGCAGATACAACAGTAGTGTTATATGGAGCAGATTCTATGCCTTCATTTAGAGT
AGCAGCAATATTAAAATATATGGGTGTTAAAGATGTAAGAGTTTTAAATGGTGGTACAGCTGCTTGGACTAGTGCTGGAT
ATGAGCTAGATACAAAAGTAAATAAGAAAACTCCAGTAGATTCTTTTGGAGTAAAGGTACCTCTAAATAAAGTATATATA
GTAGATTTACCTGAAGCTAAAGAAATATTAAAAGATAAAGAAAGAAGTAAACTTGTTGATATAAGAAGTTGGGATGAATT
TATTGGAAAAATTTCAGGTTATGATTACATAAAAGCAAAAGGTCGTCCAGCTGGAGCCGTATGGGGACACGCAGGATCAG
ACAGTTCACATTTAGAAGATTTTAGAAATGTAGATAATACTATGAGAAATTCATCAGAAATATTAGCTATGTGGGAAAAA
GAAGGTATAAAACCAGATCAAAGATTATCTTTTTATTGTGGAACTGGCTGGAGAGCAGCCGAAGTTTTAATATACGCTGA
TGTTATGGGATTAAAAAATATATCTTTATATGATGGTGGATGGAATGAATGGAGCGCGAATAAAAATAATCCAATAGAGG
TAGGAGAGCCATCTAAAAAATAG

Upstream 100 bases:

>100_bases
CTATAATTATTTTTTATTTTAAAATATAGATTAAATATCGTAAAATGTAAAATATAATGAATACAAGATTGAAATTTAGA
CATGAAAAAGAGGTGTAAAA

Downstream 100 bases:

>100_bases
TAACTATATATCTACAATAATTTTAATTACTAAAAGAGTGTGTCTTAAAATAAATAGAGATTTAATTTTTATGAAATATA
TTTTATTAGAGCGGTTTATT

Product: putative thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKKNLKGIVLLLFGIMMFTLIGCTSNQKTEEKKESTPLGKKIKTDELKKNITSKEWVVVDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYSNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTKVNKKTPVDSFGVKVPLNKVYI
VDLPEAKEILKDKERSKLVDIRSWDEFIGKISGYDYIKAKGRPAGAVWGHAGSDSSHLEDFRNVDNTMRNSSEILAMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSANKNNPIEVGEPSKK

Sequences:

>Translated_460_residues
MKKNLKGIVLLLFGIMMFTLIGCTSNQKTEEKKESTPLGKKIKTDELKKNITSKEWVVVDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYSNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTKVNKKTPVDSFGVKVPLNKVYI
VDLPEAKEILKDKERSKLVDIRSWDEFIGKISGYDYIKAKGRPAGAVWGHAGSDSSHLEDFRNVDNTMRNSSEILAMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSANKNNPIEVGEPSKK
>Mature_460_residues
MKKNLKGIVLLLFGIMMFTLIGCTSNQKTEEKKESTPLGKKIKTDELKKNITSKEWVVVDTRSNDAFNGWKLDGVKRGGH
IKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNVVLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLA
MESYSNYQMLVPASWVNDLINNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTKVNKKTPVDSFGVKVPLNKVYI
VDLPEAKEILKDKERSKLVDIRSWDEFIGKISGYDYIKAKGRPAGAVWGHAGSDSSHLEDFRNVDNTMRNSSEILAMWEK
EGIKPDQRLSFYCGTGWRAAEVLIYADVMGLKNISLYDGGWNEWSANKNNPIEVGEPSKK

Specific function: Unknown

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI17402865, Length=287, Percent_Identity=26.4808362369338, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI194473668, Length=298, Percent_Identity=24.496644295302, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI61835204, Length=295, Percent_Identity=23.728813559322, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI194473681, Length=295, Percent_Identity=23.728813559322, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI87081967, Length=446, Percent_Identity=39.6860986547085, Blast_Score=320, Evalue=8e-89,
Organism=Escherichia coli, GI87082121, Length=267, Percent_Identity=25.4681647940075, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 51871; Mature: 51871

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNLKGIVLLLFGIMMFTLIGCTSNQKTEEKKESTPLGKKIKTDELKKNITSKEWVVVD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEE
TRSNDAFNGWKLDGVKRGGHIKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCE
VLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLAMESYSNYQMLVPASWVNDLI
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHH
NNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTKV
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCC
NKKTPVDSFGVKVPLNKVYIVDLPEAKEILKDKERSKLVDIRSWDEFIGKISGYDYIKAK
CCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHEECHHHHHHHHHHCCCCEEEEEC
GRPAGAVWGHAGSDSSHLEDFRNVDNTMRNSSEILAMWEKEGIKPDQRLSFYCGTGWRAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCEEEEECCCCCHH
EVLIYADVMGLKNISLYDGGWNEWSANKNNPIEVGEPSKK
EEEEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKNLKGIVLLLFGIMMFTLIGCTSNQKTEEKKESTPLGKKIKTDELKKNITSKEWVVVD
CCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHCCCCCCEEEEE
TRSNDAFNGWKLDGVKRGGHIKGATDFSANWLKVEDTEKNKEKRLEEALKNKGITKDKNV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCE
VLYDANGKDAEEVAKYLNKKGITKLYKYDVKEWAEDDKLAMESYSNYQMLVPASWVNDLI
EEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEEHHHHHHHH
NNKKPETFKGDKYKVFEVSWGDESKAEDYKKGHIKGAVHINTDEVEKGPVWNRLPDKDLE
CCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCHHHHH
KFAKNNGITADTTVVLYGADSMPSFRVAAILKYMGVKDVRVLNGGTAAWTSAGYELDTKV
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCEEEECCC
NKKTPVDSFGVKVPLNKVYIVDLPEAKEILKDKERSKLVDIRSWDEFIGKISGYDYIKAK
CCCCCCCCCCEEECCCEEEEEECCHHHHHHHHHHHHHEECHHHHHHHHHHCCCCEEEEEC
GRPAGAVWGHAGSDSSHLEDFRNVDNTMRNSSEILAMWEKEGIKPDQRLSFYCGTGWRAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEHHHCCCCCCCCEEEEECCCCCHH
EVLIYADVMGLKNISLYDGGWNEWSANKNNPIEVGEPSKK
EEEEEEEHHCCCCEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 9097039 [H]