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The map label for this gene is ykfA [H]

Identifier: 226947274

GI number: 226947274

Start: 76590

End: 77498

Strand: Reverse

Name: ykfA [H]

Synonym: CLM_0091

Alternate gene names: 226947274

Gene position: 77498-76590 (Counterclockwise)

Preceding gene: 226947275

Following gene: 226947271

Centisome position: 1.87

GC content: 27.61

Gene sequence:

>909_bases
ATGTTATTAAAGCATTTAAACAAAGGAGATACTATAGGAATAGTTTCTCCTGCAAGTCCTATTGAGAAAGAAGTTGTTTT
AAAAAATATACAAGTATTTAAAAACTTAGGCTTTAATATTAAGCTAAGTAACCATGTGTATGATAAATATGGTTATTTAT
CCGGCAAGGATATAGATAGAGCTAACGACTTAATGAATATGTTTATAGATCCATCTGTAGATATGATTCTTTGCTCCAGA
GGGGGCTATGGCTCTATGAGAATACTTCCTTATTTAGACTTTAATATTATAAAAAATAACCCTAAAATTTTTGGTGGTTT
TAGTGATATAACAGTTCTGCTAAATTATATAACTTCTAAATGTGGCTTTACTACTTTTCACTGTCCTATGCTTTCATCAA
ACTTTAAGAATATATATACATTTAAAAATTTTCTAGAGCTATTAATGAATGATTTCACGTCTTATGAAATATCAAATCCT
AATTTTGTTCCTTTATTATCTCAAACTAATGATATAGTAGAAGGCAATCTTGTTGGTGGAAATTTATCTCTCATATGCAA
TACCTTAGGTACTCCCTATGAGATTAATACTATGGATAATATTTTATTCTTAGAAGAAATTTCAGAGCCGCCTTATAAAA
TAGATAGGATGTTAACTCAATTAATACTATCTGGAAAAATTAAATCCTGTTCTGGACTTATATTAGGTCAATTTACAAAT
TGTAATATTGATAATTGCGAAAAAGATTTCTCTCTAGATGAAGTTATATTAGATAGGTTATTGTCTAGTAATAAACCTGT
TATATCAAACTTAATGTCAGGGCACTGTGATCCTAATGTAACCTTACCTATAGGATCTAAAATTAGATTGGATTGTAAAA
ACAAGTTAATAAAAATTCTAAATTATTAA

Upstream 100 bases:

>100_bases
GTGGAGTTACAGTTATATTATGTATAGTTTGTATAATAATATTGAAAAAAAAGGACTTATTCTAATGAATTACATATAAT
TAATTATTAGGAGTGATTTA

Downstream 100 bases:

>100_bases
AAAAGGAGTTTTAGTATCCTAAAACTCCTTTGGAGGCGCCACCCAGATTTGAACTGGGGATAAAGGCTTTGCAGGCCTCT
GCCTTACCACTTGGCTATAG

Product: LD-carboxypeptidase family protein

Products: NA

Alternate protein names: LD-carboxypeptidase [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLLKHLNKGDTIGIVSPASPIEKEVVLKNIQVFKNLGFNIKLSNHVYDKYGYLSGKDIDRANDLMNMFIDPSVDMILCSR
GGYGSMRILPYLDFNIIKNNPKIFGGFSDITVLLNYITSKCGFTTFHCPMLSSNFKNIYTFKNFLELLMNDFTSYEISNP
NFVPLLSQTNDIVEGNLVGGNLSLICNTLGTPYEINTMDNILFLEEISEPPYKIDRMLTQLILSGKIKSCSGLILGQFTN
CNIDNCEKDFSLDEVILDRLLSSNKPVISNLMSGHCDPNVTLPIGSKIRLDCKNKLIKILNY

Sequences:

>Translated_302_residues
MLLKHLNKGDTIGIVSPASPIEKEVVLKNIQVFKNLGFNIKLSNHVYDKYGYLSGKDIDRANDLMNMFIDPSVDMILCSR
GGYGSMRILPYLDFNIIKNNPKIFGGFSDITVLLNYITSKCGFTTFHCPMLSSNFKNIYTFKNFLELLMNDFTSYEISNP
NFVPLLSQTNDIVEGNLVGGNLSLICNTLGTPYEINTMDNILFLEEISEPPYKIDRMLTQLILSGKIKSCSGLILGQFTN
CNIDNCEKDFSLDEVILDRLLSSNKPVISNLMSGHCDPNVTLPIGSKIRLDCKNKLIKILNY
>Mature_302_residues
MLLKHLNKGDTIGIVSPASPIEKEVVLKNIQVFKNLGFNIKLSNHVYDKYGYLSGKDIDRANDLMNMFIDPSVDMILCSR
GGYGSMRILPYLDFNIIKNNPKIFGGFSDITVLLNYITSKCGFTTFHCPMLSSNFKNIYTFKNFLELLMNDFTSYEISNP
NFVPLLSQTNDIVEGNLVGGNLSLICNTLGTPYEINTMDNILFLEEISEPPYKIDRMLTQLILSGKIKSCSGLILGQFTN
CNIDNCEKDFSLDEVILDRLLSSNKPVISNLMSGHCDPNVTLPIGSKIRLDCKNKLIKILNY

Specific function: May be involved in the degradation of peptidoglycan by catalyzing the cleavage of the terminal D-alanine residue from cytoplasmic murein peptides [H]

COG id: COG1619

COG function: function code V; Uncharacterized proteins, homologs of microcin C7 resistance protein MccF

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S66 family [H]

Homologues:

Organism=Escherichia coli, GI1787441, Length=277, Percent_Identity=27.7978339350181, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003507 [H]

Pfam domain/function: PF02016 Peptidase_S66 [H]

EC number: NA

Molecular weight: Translated: 33929; Mature: 33929

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKHLNKGDTIGIVSPASPIEKEVVLKNIQVFKNLGFNIKLSNHVYDKYGYLSGKDIDR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCHH
ANDLMNMFIDPSVDMILCSRGGYGSMRILPYLDFNIIKNNPKIFGGFSDITVLLNYITSK
HHHHHHHHCCCCCCEEEECCCCCCCEEEEEEEEEEEEECCCEEEECHHHHHHHHHHHHHH
CGFTTFHCPMLSSNFKNIYTFKNFLELLMNDFTSYEISNPNFVPLLSQTNDIVEGNLVGG
CCCEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCCCEECEEECC
NLSLICNTLGTPYEINTMDNILFLEEISEPPYKIDRMLTQLILSGKIKSCSGLILGQFTN
CCEEHEECCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
CNIDNCEKDFSLDEVILDRLLSSNKPVISNLMSGHCDPNVTLPIGSKIRLDCKNKLIKIL
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCEEEECHHHHHHHHH
NY
CC
>Mature Secondary Structure
MLLKHLNKGDTIGIVSPASPIEKEVVLKNIQVFKNLGFNIKLSNHVYDKYGYLSGKDIDR
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCCCCCCCHH
ANDLMNMFIDPSVDMILCSRGGYGSMRILPYLDFNIIKNNPKIFGGFSDITVLLNYITSK
HHHHHHHHCCCCCCEEEECCCCCCCEEEEEEEEEEEEECCCEEEECHHHHHHHHHHHHHH
CGFTTFHCPMLSSNFKNIYTFKNFLELLMNDFTSYEISNPNFVPLLSQTNDIVEGNLVGG
CCCEEEECCHHHCCCHHHHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCCCEECEEECC
NLSLICNTLGTPYEINTMDNILFLEEISEPPYKIDRMLTQLILSGKIKSCSGLILGQFTN
CCEEHEECCCCCEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECC
CNIDNCEKDFSLDEVILDRLLSSNKPVISNLMSGHCDPNVTLPIGSKIRLDCKNKLIKIL
CCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEECCCCEEEECHHHHHHHHH
NY
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]