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The map label for this gene is cobB [H]

Identifier: 226947273

GI number: 226947273

Start: 75661

End: 76404

Strand: Direct

Name: cobB [H]

Synonym: CLM_0089

Alternate gene names: 226947273

Gene position: 75661-76404 (Clockwise)

Preceding gene: 226947272

Following gene: 226947276

Centisome position: 1.82

GC content: 27.02

Gene sequence:

>744_bases
ATGAGGCTAGAAAAATTAAAACATATTATAGATAATAGTGAAAATATAGTGTTTTTAGGTGGAGCAGGAGTGTCTACAGA
AAGTAATATTCCGGATTTCAGATCAAACAATGGATTATACAAATCAAAAAATAATTTGAATTATTCACCAGAAACAATAT
TAAGTCATAGTTTTTTTAAGAATAATACTAAAGAATTTTTTCAATTTTATAAAGATAAGATGATATTTAAAGATGCAAAG
CCTAATTTAGCCCATTATGCTTTAACAGAGTTAGAACAGATAGGAAAACTAAAGGCTATAATAACGCAAAATATTGATGG
ATTACATCAGTTATCAGGAGCTAAGAATGTATTAGAGTTACATGGAAGTGTGCATAGAAATTATTGTGTTAATTGTGGAG
AAAAATATAATTTAGATTATATATTAAATACTGAAAATAGTAGTGAAGATATACCGCATTGTAAAAAATGTGGAAGTATA
GTTAGACCAGATGTAGTGCTTTATGAAGAAGGATTAGACATGGATACTATAAACAAAGCCGTATATTATATACAAAATGC
AGATGTGCTTATAGTAGGTGGTACATCACTTGTAGTATATCCTGCTGCTGGATTAGTTAATTATTATAAGGGTAAAAAAT
TAGTTTTAATAAATAAAGCAGAAACACCTTATGACAAAAAAGCTGACTTAGTTATTCATGATTCTATAGGTAGTGTTTTA
GAAAAAGTAATTAAAAAAAACTAA

Upstream 100 bases:

>100_bases
TTTATAGTTCTTATTTCTTCTTTAGGAGTAATTAAGTTTTAATAATTGTATTATTAGTATATAATAATTATGATTAATAA
CCTATTTAGGAGATGATAAT

Downstream 100 bases:

>100_bases
TCAAATTTTTGGTTAGTTTTTTAATTTTAATATTGACAAGTTTTAAATTTCTGATATAATAAATTATGTTGAATTGAATA
TGGCGCTATAGCCAAGTGGT

Product: NAD-dependent deacetylase

Products: NA

Alternate protein names: Regulatory protein SIR2 homolog [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTINKAVYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN

Sequences:

>Translated_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTINKAVYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN
>Mature_247_residues
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFKNNTKEFFQFYKDKMIFKDAK
PNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLELHGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSI
VRPDVVLYEEGLDMDTINKAVYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EKVIKKN

Specific function: Modulates the activities of several enzymes which are inactive in their acetylated form [H]

COG id: COG0846

COG function: function code K; NAD-dependent protein deacetylases, SIR2 family

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 deacetylase sirtuin-type domain [H]

Homologues:

Organism=Homo sapiens, GI6912662, Length=272, Percent_Identity=28.6764705882353, Blast_Score=124, Evalue=7e-29,
Organism=Homo sapiens, GI6912664, Length=267, Percent_Identity=28.4644194756554, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI13787215, Length=246, Percent_Identity=30.0813008130081, Blast_Score=120, Evalue=8e-28,
Organism=Homo sapiens, GI300795542, Length=253, Percent_Identity=29.2490118577075, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI6912660, Length=212, Percent_Identity=36.3207547169811, Blast_Score=118, Evalue=4e-27,
Organism=Homo sapiens, GI300797577, Length=256, Percent_Identity=29.296875, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI13775602, Length=217, Percent_Identity=35.0230414746544, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI13775600, Length=217, Percent_Identity=35.0230414746544, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI63054862, Length=205, Percent_Identity=37.0731707317073, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI300797597, Length=250, Percent_Identity=29.6, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI300797705, Length=194, Percent_Identity=35.0515463917526, Blast_Score=109, Evalue=2e-24,
Organism=Homo sapiens, GI7657575, Length=234, Percent_Identity=29.0598290598291, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI215982798, Length=175, Percent_Identity=29.1428571428571, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI308199517, Length=201, Percent_Identity=33.8308457711443, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17541892, Length=216, Percent_Identity=33.3333333333333, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71990482, Length=281, Percent_Identity=29.1814946619217, Blast_Score=113, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17567771, Length=276, Percent_Identity=30.0724637681159, Blast_Score=110, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71990487, Length=283, Percent_Identity=28.9752650176678, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17505510, Length=241, Percent_Identity=28.2157676348548, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6325242, Length=257, Percent_Identity=31.5175097276265, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324599, Length=295, Percent_Identity=27.7966101694915, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320163, Length=251, Percent_Identity=27.4900398406374, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324504, Length=252, Percent_Identity=28.1746031746032, Blast_Score=68, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648389, Length=210, Percent_Identity=36.1904761904762, Blast_Score=126, Evalue=1e-29,
Organism=Drosophila melanogaster, GI28571445, Length=276, Percent_Identity=28.2608695652174, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137536, Length=223, Percent_Identity=30.4932735426009, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24645650, Length=257, Percent_Identity=29.9610894941634, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28571443, Length=197, Percent_Identity=27.9187817258883, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI28571441, Length=197, Percent_Identity=27.9187817258883, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003000 [H]

Pfam domain/function: PF02146 SIR2 [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 27779; Mature: 27779

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50305 SIRTUIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHC
NNTKEFFQFYKDKMIFKDAKPNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSIVRPDVVLYEEGLDMDTINKA
HHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHCCE
VYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EEEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCHHHHH
EKVIKKN
HHHHHCC
>Mature Secondary Structure
MRLEKLKHIIDNSENIVFLGGAGVSTESNIPDFRSNNGLYKSKNNLNYSPETILSHSFFK
CCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHC
NNTKEFFQFYKDKMIFKDAKPNLAHYALTELEQIGKLKAIITQNIDGLHQLSGAKNVLEL
CCHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHH
HGSVHRNYCVNCGEKYNLDYILNTENSSEDIPHCKKCGSIVRPDVVLYEEGLDMDTINKA
HHHHCCCHHCCCCCCCCCEEEEECCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHCCE
VYYIQNADVLIVGGTSLVVYPAAGLVNYYKGKKLVLINKAETPYDKKADLVIHDSIGSVL
EEEEECCCEEEECCCEEEEECCHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCHHHHH
EKVIKKN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]