Click here to switch to the map view.

The map label for this gene is recF

Identifier: 226947226

GI number: 226947226

Start: 3164

End: 4258

Strand: Direct

Name: recF

Synonym: CLM_0004

Alternate gene names: 226947226

Gene position: 3164-4258 (Clockwise)

Preceding gene: 226947225

Following gene: 226947227

Centisome position: 0.08

GC content: 22.92

Gene sequence:

>1095_bases
ATGTATATAAAAAATGTGCACTTAATAAATTTTAGAAATTATGATGATATGTATTTAGAATTAAGTCCAAATACAAATAT
TTTTGTAGGTAATAATGCTCAAGGGAAAACTAATATACTAGAAAGTATATATTATTCAAGTATAGGAAAGTCTCATAGAA
CAAATAAAGATAAAGATCTTATAAAATGGGATAAAAATAATACATACTTAAGGACATATGTATCCAGGGAAAGATTAGAT
AAAACTATAGATATAAATATATTTAAAAATGGCAAAAAAGCTATAACAGTAAATAAAATAAAAATAAAAAAAATATCTGA
ACTAATGGGGAACTTAAATGTTGTTATGTTTTCGCCAGAAGACTTAAGAATAATTAAGGATTCCCCTGGAAATAGAAGGA
AATTTCTAGATATAGAATTATGTAAAATAAACAATGTTTATTATCATGATTTAGTCCAATATAATAAAATTTTATCTGAA
AGAAATACAGCTTTAAAAAATTGGAATAACAAAATTAATGATATAATTGATATTTATGATGAGCAACTTTCTAAATATGG
AGCTTTTATAATTAAAGAGAGAAATAAGTATTTGGATAAATTAAATATTATTGGCAAAAATATACATAAGAAGATAACCA
ATGATTTAGAAGACATAAATTTTAGATATTTAACTAATATAAAAGATTTTGATAATGCAGAAAAAGAATTATTAATAGTT
CTTAAGAAAAATCGAAAAAAAGATCTTGAGAGAAATTCAACCTCTATTGGTCCTCACCGAGATGATTTTGAAGTAAGTAT
AAACAATATAGATACTAGAATTTTTGGATCACAAGGACAACAAAGAACAGCTGTATTAACTTTAAAGTTTGCATCATTGG
AAATAATAAAAAACATAATAGGGGAATATCCTGTGCTTTTATTAGATGATGTACTATCTGAATTAGATTCAAATAGACAG
AAGTTTGTATTGAATTCTATTGATAAAATACAAACTATAATAACTTGTACTGGTATAGAAGAAATAGACAAATACTTAGA
TAAGAAACAATCTCAATTATATTTAGTTAATAATGGTAAAATAAAAAGAGTTTAA

Upstream 100 bases:

>100_bases
TGAAACATATAAAATTATTTAAAATTATTTAAAATTTTATATAAATTTTAAATAATTGTTACTAAAAAATATTATGGTAT
AATTAATTTAGGTGTTTAGA

Downstream 100 bases:

>100_bases
AGCTGAAAGGAGATTAATTTATGTTTCTTCACTTAGGAGAAAATGTAGTAGTACCAATAAAAGATGTTATAGGAATATTC
GACATGGAAACTTCTATGTA

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD
KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE
RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKELLIV
LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ
KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV

Sequences:

>Translated_364_residues
MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD
KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE
RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKELLIV
LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ
KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV
>Mature_364_residues
MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDLIKWDKNNTYLRTYVSRERLD
KTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPEDLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSE
RNTALKNWNNKINDIIDIYDEQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKELLIV
LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNIIGEYPVLLLDDVLSELDSNRQ
KFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGKIKRV

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=370, Percent_Identity=24.0540540540541, Blast_Score=113, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_CLOB1 (A7FPF3)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382414.1
- ProteinModelPortal:   A7FPF3
- SMR:   A7FPF3
- STRING:   A7FPF3
- GeneID:   5397407
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0004
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   CBOT441770:CLB_0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42642; Mature: 42642

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDL
CEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPE
EEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEECCC
DLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIIDIYD
CEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKELLIV
HHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEEEEE
LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNII
EECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHHHHH
GEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEECCE
IKRV
EECC
>Mature Secondary Structure
MYIKNVHLINFRNYDDMYLELSPNTNIFVGNNAQGKTNILESIYYSSIGKSHRTNKDKDL
CEEEEEEEEEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
IKWDKNNTYLRTYVSRERLDKTIDINIFKNGKKAITVNKIKIKKISELMGNLNVVMFSPE
EEECCCCCEEEEHHHHHHCCCEEEEEEEECCCEEEEEEEEEHHHHHHHHCCCEEEEECCC
DLRIIKDSPGNRRKFLDIELCKINNVYYHDLVQYNKILSERNTALKNWNNKINDIIDIYD
CEEEEECCCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EQLSKYGAFIIKERNKYLDKLNIIGKNIHKKITNDLEDINFRYLTNIKDFDNAEKELLIV
HHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEEECCCCCCCCCEEEEEE
LKKNRKKDLERNSTSIGPHRDDFEVSINNIDTRIFGSQGQQRTAVLTLKFASLEIIKNII
EECCCHHHHHCCCCCCCCCCCCCEEEEECCEEEEECCCCCCEEEEEEEEHHHHHHHHHHH
GEYPVLLLDDVLSELDSNRQKFVLNSIDKIQTIITCTGIEEIDKYLDKKQSQLYLVNNGK
CCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEECCHHHHHHHHCCCCCEEEEEECCE
IKRV
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA