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The map label for this gene is thrB

Identifier: 226225093

GI number: 226225093

Start: 2593941

End: 2594807

Strand: Reverse

Name: thrB

Synonym: Lm4b_02514

Alternate gene names: 226225093

Gene position: 2594807-2593941 (Counterclockwise)

Preceding gene: 226225094

Following gene: 226225092

Centisome position: 89.09

GC content: 44.98

Gene sequence:

>867_bases
ATGCGTATTCGTGTACCGGCAACAACGGCCAATCTTGGCCCTGGCTTTGATTCGTGTGGTTTAGCGTTAACGTTATATTT
AACGCTTGATATTGGAGCGGAAGCAGACTCTTGGTATATCGAACATAATATTGGCGGCGGGATTCCACATGATGAAACCA
ATGTAATTATCGAAACGGCCTTAAACCTGGCGCCTAATTTAACGCCGCATCATTTAGTGATGACCTGTGATATACCGCCA
GCCCGTGGACTTGGTAGTAGTTCTGCAGCGGTTGTTGCGGGGATTGAATTAGCCAATACACTTGCTGAACTTAAACTTTC
AAAAGAGGAAAAAGTTCGGATAGCTGCTGAAATAGAAGGGCATCCCGATAATGTTGCGCCTGCTGTTCTTGGAAACTGGG
TCGTAGGAGCGAAATTAGATGGAGAAGATTTCTATGTACGCCACCTTTTTCCGGATTGTGCTTTAATTGCTTTTATTCCA
AAGGCAGAACTTCTTACTTCGGAAAGTCGCGGTGTTTTGCCTGATACGCTTCCATTCAAAGAAGCTGTCCAAGCGAGCAG
TATCGCCAATGTAATGATTGCAGCAATTTTACGCAATGATATGGCGCTAGCTGGTGAAATGATGGAACGTGATTTATGGC
ATGAAAAATATCGCAGTCAATTAGTGCCACACTTAACACAAATTCGCGATGTTGCAAAAAGCCAAGGAGCCTATGCTGCT
TGTTTGAGCGGCGCGGGCCCAACTGTCTTAGTGTTCGCTCCAAGAAACCTCGCTAACACACTGCAAACATCTTTACAAAC
ACTGGAAATCGATGCAGACGTTCTTCTTCTTGATGTCGAAGGGAGCGGCGCAGAAGTTTTTCGTTAA

Upstream 100 bases:

>100_bases
AAGATCCGGATACTGCGATGAGTGTGCATGAAATTCCAATTTCTCATGTGGATGATATCGAAGCAATGCGCACACATTTA
CGTTCAGGAGTGAAAGCTTA

Downstream 100 bases:

>100_bases
AATCAAGCTATGCCCCTATGCGGCGTAGCTTTTTTTAACTATATGTCCCAATTTGTGCCGCTCTATGATAAAATAGGAAA
AGAGTTTATAAAGTTAAAGG

Product: homoserine kinase

Products: NA

Alternate protein names: HK; HSK [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIGGGIPHDETNVIIETALNLAPNLTPHHLVMTCDIPP
ARGLGSSSAAVVAGIELANTLAELKLSKEEKVRIAAEIEGHPDNVAPAVLGNWVVGAKLDGEDFYVRHLFPDCALIAFIP
KAELLTSESRGVLPDTLPFKEAVQASSIANVMIAAILRNDMALAGEMMERDLWHEKYRSQLVPHLTQIRDVAKSQGAYAA
CLSGAGPTVLVFAPRNLANTLQTSLQTLEIDADVLLLDVEGSGAEVFR

Sequences:

>Translated_288_residues
MRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIGGGIPHDETNVIIETALNLAPNLTPHHLVMTCDIPP
ARGLGSSSAAVVAGIELANTLAELKLSKEEKVRIAAEIEGHPDNVAPAVLGNWVVGAKLDGEDFYVRHLFPDCALIAFIP
KAELLTSESRGVLPDTLPFKEAVQASSIANVMIAAILRNDMALAGEMMERDLWHEKYRSQLVPHLTQIRDVAKSQGAYAA
CLSGAGPTVLVFAPRNLANTLQTSLQTLEIDADVLLLDVEGSGAEVFR
>Mature_288_residues
MRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIGGGIPHDETNVIIETALNLAPNLTPHHLVMTCDIPP
ARGLGSSSAAVVAGIELANTLAELKLSKEEKVRIAAEIEGHPDNVAPAVLGNWVVGAKLDGEDFYVRHLFPDCALIAFIP
KAELLTSESRGVLPDTLPFKEAVQASSIANVMIAAILRNDMALAGEMMERDLWHEKYRSQLVPHLTQIRDVAKSQGAYAA
CLSGAGPTVLVFAPRNLANTLQTSLQTLEIDADVLLLDVEGSGAEVFR

Specific function: Catalyzes the ATP-dependent phosphorylation of L- homoserine to L-homoserine phosphate [H]

COG id: COG0083

COG function: function code E; Homoserine kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. Homoserine kinase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786184, Length=307, Percent_Identity=25.4071661237785, Blast_Score=78, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321814, Length=326, Percent_Identity=31.2883435582822, Blast_Score=121, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR006203
- InterPro:   IPR000870
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.39 [H]

Molecular weight: Translated: 30793; Mature: 30793

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00627 GHMP_KINASES_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIGGGIPHDETNVIIETA
CEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEEH
LNLAPNLTPHHLVMTCDIPPARGLGSSSAAVVAGIELANTLAELKLSKEEKVRIAAEIEG
HHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECC
HPDNVAPAVLGNWVVGAKLDGEDFYVRHLFPDCALIAFIPKAELLTSESRGVLPDTLPFK
CCCCCCHHHHCCEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCHH
EAVQASSIANVMIAAILRNDMALAGEMMERDLWHEKYRSQLVPHLTQIRDVAKSQGAYAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CLSGAGPTVLVFAPRNLANTLQTSLQTLEIDADVLLLDVEGSGAEVFR
EECCCCCEEEEECCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCC
>Mature Secondary Structure
MRIRVPATTANLGPGFDSCGLALTLYLTLDIGAEADSWYIEHNIGGGIPHDETNVIIETA
CEEECCCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCEEEEECCCCCCCCCCCCEEEEEH
LNLAPNLTPHHLVMTCDIPPARGLGSSSAAVVAGIELANTLAELKLSKEEKVRIAAEIEG
HHCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCEEEEEEECC
HPDNVAPAVLGNWVVGAKLDGEDFYVRHLFPDCALIAFIPKAELLTSESRGVLPDTLPFK
CCCCCCHHHHCCEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHCCCCCCCCCCCCCHH
EAVQASSIANVMIAAILRNDMALAGEMMERDLWHEKYRSQLVPHLTQIRDVAKSQGAYAA
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
CLSGAGPTVLVFAPRNLANTLQTSLQTLEIDADVLLLDVEGSGAEVFR
EECCCCCEEEEECCCHHHHHHHHHHHEEEECCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]