Click here to switch to the map view.

The map label for this gene is phoR

Identifier: 226225048

GI number: 226225048

Start: 2547797

End: 2549572

Strand: Reverse

Name: phoR

Synonym: Lm4b_02469

Alternate gene names: 226225048

Gene position: 2549572-2547797 (Counterclockwise)

Preceding gene: 226225049

Following gene: 226225047

Centisome position: 87.53

GC content: 38.74

Gene sequence:

>1776_bases
ATGAAGAAATTATGGCTGAAAATCGGGTTATCCTTTTTTATCTTATTTTTCGTTGTGATGGTTATTGTTGGCGTTTTTTC
CGGGGAACTAATGAAATCAACGTACTTAAATATGAAAGAAAATCAATTAGAAGATGACGCAAAAATTCTGCTTCAAACAA
CTAATATGGAGAATTTAGATTTAGATAAAGATGCCGCAACTATCCAAAAAAGTCTTGATCCACTTGGCGAGGATATTGAT
GCACGCATTACGGTGATCGATAGCAAAGGCGACGTGGTAGCTGATACAAAAAAAGACCCAGAAAAACTTGATAACCATAT
GAATCGCCCCGAAGTAACAGATATTCTGAAAAAAGGGGAAAGCGTTGGTATTTCAATCCGTGAAAGTGATTCACTTGGTT
ATAGTATGCTTTATGTCGCGGTTCCTGTTAAACATCAAGGCAAAACAGATGGTGTACTGAGAATTTCGATTTCACTTGAA
TCTGTTGATGCGGCGGTTGCTAAACTCTGGGGGAACTTAGCGCTTATTTTCGGTATTGCACTTGTAATTATCGCAGCAAT
CAGTGTTTTTATTGCTAGAAAAATCACTAGACCAGTTCGCGAAATCATCGAAGTCTCTACAGATTTGGCCAATCATAAAT
ACGATAGTCGCATCCACGGTAAAATAAGCGGAGAACTTCAAGATCTCTCCATCAGTGTAAACACATTAGCAGAAAGCTTG
GAAACGCAAATGTTTGAAATTAAGCAAAATGAGCAACGACTTAATGCTATTGTCCAAAACTTAGTAAGTGGCGTAATGCT
GATTAATGTCGATAAACAAGTCATCATGACCAACCGCACCATGTATCAAATTTTAGGAGAGACAGAAATCACCGGCAAAC
CATTTTATGAAGTAATTAAAAGTTTTGCACTGAGCCAGTTGATAGAAGCGACTTTTGAAACAAAAACCATTCAACAAAAA
GAAATTATTCTTTATTTCCCGCGCGAAATGATTTTGGATGCAAGTGTTTCTCCAATTTTAGGGGAGAATGGGGAAATTAC
TGGGATTATTTTATTACTGCATGATATCACTCAAATTCGTCATTTGGAAAATGTTCGTTCTGAATTTGTCACCAACGTTT
CTCATGAATTAAAAACACCCGTGACGGCTCTCAAAGGATTCGCGGAAACATTACTTGACGGCGCAATGTACGACGAAGTG
CTACTAAAAAAATTCCTAACGATTATTAAAGAAGAAAGTGATCGATTGCACCGTTTGATTATGGATATTCTCGCACTTTC
TAGAATTGAACAAAATCCGGTCGCGGAAAACGTTGAGCTGGTTGACGTTGACGAGGTAATTGAACAATCTGCTCGCACTA
TTTTTGAAATGGCGACCGAAAAAAATATTCGAGTAACCATTCCAGAAAAAACAAGTGCATCCGTTATGATTGAAACAGAT
CGTGACAAGCTGCAACAGATAGTAATTAACTTGCTTTCTAATGCAATCAACTACACCCCAGTCGATGGAAAAGTAGAAGT
TAAATTAATCGAGCAAGAGGCAGAAGTTATAATAGAAGTAACTGATAACGGAATTGGCATCCCGGCTAAAGATATTGACC
GCGTGTTTGAACGATTCTACCGAGTGGATAAAGCCCGCAGCAGACACTCAGGAGGCACTGGACTTGGACTTTCCATCGTC
AAGCATTTAGTTGAAAACTGTGGTGGGCGAATCGAAGTAGAAAGCCAAGAAGAAGTTGGCTCGACCTTCCGCGTCACTTT
ACCAAAAAAAGCCTAA

Upstream 100 bases:

>100_bases
ATGTCAGCCATTTGCGCGATAAAATCGAACTAGATACAAAACAACCTAAATACATCAAAACAATTCGTGGCTTCGGTTAT
AAAATGGAGAACGTGAAATA

Downstream 100 bases:

>100_bases
CAAATATCCTGTTTGAAAGAATGTAATCTTTCAGACAGGATATTTTTATTTTATTTACGAAAGAAACAAATTTGGTGTAA
TAATTTACTAGTTTGCCTTC

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 591; Mature: 591

Protein sequence:

>591_residues
MKKLWLKIGLSFFILFFVVMVIVGVFSGELMKSTYLNMKENQLEDDAKILLQTTNMENLDLDKDAATIQKSLDPLGEDID
ARITVIDSKGDVVADTKKDPEKLDNHMNRPEVTDILKKGESVGISIRESDSLGYSMLYVAVPVKHQGKTDGVLRISISLE
SVDAAVAKLWGNLALIFGIALVIIAAISVFIARKITRPVREIIEVSTDLANHKYDSRIHGKISGELQDLSISVNTLAESL
ETQMFEIKQNEQRLNAIVQNLVSGVMLINVDKQVIMTNRTMYQILGETEITGKPFYEVIKSFALSQLIEATFETKTIQQK
EIILYFPREMILDASVSPILGENGEITGIILLLHDITQIRHLENVRSEFVTNVSHELKTPVTALKGFAETLLDGAMYDEV
LLKKFLTIIKEESDRLHRLIMDILALSRIEQNPVAENVELVDVDEVIEQSARTIFEMATEKNIRVTIPEKTSASVMIETD
RDKLQQIVINLLSNAINYTPVDGKVEVKLIEQEAEVIIEVTDNGIGIPAKDIDRVFERFYRVDKARSRHSGGTGLGLSIV
KHLVENCGGRIEVESQEEVGSTFRVTLPKKA

Sequences:

>Translated_591_residues
MKKLWLKIGLSFFILFFVVMVIVGVFSGELMKSTYLNMKENQLEDDAKILLQTTNMENLDLDKDAATIQKSLDPLGEDID
ARITVIDSKGDVVADTKKDPEKLDNHMNRPEVTDILKKGESVGISIRESDSLGYSMLYVAVPVKHQGKTDGVLRISISLE
SVDAAVAKLWGNLALIFGIALVIIAAISVFIARKITRPVREIIEVSTDLANHKYDSRIHGKISGELQDLSISVNTLAESL
ETQMFEIKQNEQRLNAIVQNLVSGVMLINVDKQVIMTNRTMYQILGETEITGKPFYEVIKSFALSQLIEATFETKTIQQK
EIILYFPREMILDASVSPILGENGEITGIILLLHDITQIRHLENVRSEFVTNVSHELKTPVTALKGFAETLLDGAMYDEV
LLKKFLTIIKEESDRLHRLIMDILALSRIEQNPVAENVELVDVDEVIEQSARTIFEMATEKNIRVTIPEKTSASVMIETD
RDKLQQIVINLLSNAINYTPVDGKVEVKLIEQEAEVIIEVTDNGIGIPAKDIDRVFERFYRVDKARSRHSGGTGLGLSIV
KHLVENCGGRIEVESQEEVGSTFRVTLPKKA
>Mature_591_residues
MKKLWLKIGLSFFILFFVVMVIVGVFSGELMKSTYLNMKENQLEDDAKILLQTTNMENLDLDKDAATIQKSLDPLGEDID
ARITVIDSKGDVVADTKKDPEKLDNHMNRPEVTDILKKGESVGISIRESDSLGYSMLYVAVPVKHQGKTDGVLRISISLE
SVDAAVAKLWGNLALIFGIALVIIAAISVFIARKITRPVREIIEVSTDLANHKYDSRIHGKISGELQDLSISVNTLAESL
ETQMFEIKQNEQRLNAIVQNLVSGVMLINVDKQVIMTNRTMYQILGETEITGKPFYEVIKSFALSQLIEATFETKTIQQK
EIILYFPREMILDASVSPILGENGEITGIILLLHDITQIRHLENVRSEFVTNVSHELKTPVTALKGFAETLLDGAMYDEV
LLKKFLTIIKEESDRLHRLIMDILALSRIEQNPVAENVELVDVDEVIEQSARTIFEMATEKNIRVTIPEKTSASVMIETD
RDKLQQIVINLLSNAINYTPVDGKVEVKLIEQEAEVIIEVTDNGIGIPAKDIDRVFERFYRVDKARSRHSGGTGLGLSIV
KHLVENCGGRIEVESQEEVGSTFRVTLPKKA

Specific function: Member of the two-component regulatory system phoP/phoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates phoP in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=348, Percent_Identity=30.7471264367816, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI48994928, Length=402, Percent_Identity=25.8706467661692, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI1789149, Length=229, Percent_Identity=33.1877729257642, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1788393, Length=232, Percent_Identity=29.3103448275862, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1790436, Length=228, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1788549, Length=384, Percent_Identity=24.7395833333333, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693157, Length=224, Percent_Identity=31.6964285714286, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788713, Length=258, Percent_Identity=25.968992248062, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1786912, Length=229, Percent_Identity=29.6943231441048, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1790346, Length=236, Percent_Identity=29.2372881355932, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1786783, Length=260, Percent_Identity=27.6923076923077, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081816, Length=229, Percent_Identity=28.3842794759825, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790861, Length=222, Percent_Identity=31.0810810810811, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788279, Length=229, Percent_Identity=29.2576419213974, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1790300, Length=360, Percent_Identity=27.5, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI87082128, Length=234, Percent_Identity=29.9145299145299, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1787894, Length=224, Percent_Identity=25.4464285714286, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789403, Length=251, Percent_Identity=29.8804780876494, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1790551, Length=215, Percent_Identity=27.906976744186, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1786837, Length=619, Percent_Identity=22.1324717285945, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=235, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 66117; Mature: 66117

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS50885 HAMP ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLWLKIGLSFFILFFVVMVIVGVFSGELMKSTYLNMKENQLEDDAKILLQTTNMENLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCHHEEEEECCCCCCC
LDKDAATIQKSLDPLGEDIDARITVIDSKGDVVADTKKDPEKLDNHMNRPEVTDILKKGE
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCHHHHHHCCCCCHHHHHHHCCC
SVGISIRESDSLGYSMLYVAVPVKHQGKTDGVLRISISLESVDAAVAKLWGNLALIFGIA
CCEEEEECCCCCCEEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LVIIAAISVFIARKITRPVREIIEVSTDLANHKYDSRIHGKISGELQDLSISVNTLAESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEHEEEHHHHHHHH
ETQMFEIKQNEQRLNAIVQNLVSGVMLINVDKQVIMTNRTMYQILGETEITGKPFYEVIK
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCHHHHHHCCCCCCCCHHHHHHH
SFALSQLIEATFETKTIQQKEIILYFPREMILDASVSPILGENGEITGIILLLHDITQIR
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHH
HLENVRSEFVTNVSHELKTPVTALKGFAETLLDGAMYDEVLLKKFLTIIKEESDRLHRLI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDILALSRIEQNPVAENVELVDVDEVIEQSARTIFEMATEKNIRVTIPEKTSASVMIETD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECC
RDKLQQIVINLLSNAINYTPVDGKVEVKLIEQEAEVIIEVTDNGIGIPAKDIDRVFERFY
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
RVDKARSRHSGGTGLGLSIVKHLVENCGGRIEVESQEEVGSTFRVTLPKKA
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCCEEEEECCCCC
>Mature Secondary Structure
MKKLWLKIGLSFFILFFVVMVIVGVFSGELMKSTYLNMKENQLEDDAKILLQTTNMENLD
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHCCCCCHHEEEEECCCCCCC
LDKDAATIQKSLDPLGEDIDARITVIDSKGDVVADTKKDPEKLDNHMNRPEVTDILKKGE
CCCHHHHHHHHHHHCCCCCCCEEEEEECCCCEEECCCCCHHHHHHCCCCCHHHHHHHCCC
SVGISIRESDSLGYSMLYVAVPVKHQGKTDGVLRISISLESVDAAVAKLWGNLALIFGIA
CCEEEEECCCCCCEEEEEEEEEECCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHH
LVIIAAISVFIARKITRPVREIIEVSTDLANHKYDSRIHGKISGELQDLSISVNTLAESL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCEEEHEEEHHHHHHHH
ETQMFEIKQNEQRLNAIVQNLVSGVMLINVDKQVIMTNRTMYQILGETEITGKPFYEVIK
HHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEECCHHHHHHCCCCCCCCHHHHHHH
SFALSQLIEATFETKTIQQKEIILYFPREMILDASVSPILGENGEITGIILLLHDITQIR
HHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHH
HLENVRSEFVTNVSHELKTPVTALKGFAETLLDGAMYDEVLLKKFLTIIKEESDRLHRLI
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MDILALSRIEQNPVAENVELVDVDEVIEQSARTIFEMATEKNIRVTIPEKTSASVMIETD
HHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECC
RDKLQQIVINLLSNAINYTPVDGKVEVKLIEQEAEVIIEVTDNGIGIPAKDIDRVFERFY
HHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHH
RVDKARSRHSGGTGLGLSIVKHLVENCGGRIEVESQEEVGSTFRVTLPKKA
HHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3142862; 9387221; 9384377 [H]