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The map label for this gene is namA [H]

Identifier: 226225019

GI number: 226225019

Start: 2518224

End: 2519240

Strand: Reverse

Name: namA [H]

Synonym: Lm4b_02440

Alternate gene names: 226225019

Gene position: 2519240-2518224 (Counterclockwise)

Preceding gene: 226225020

Following gene: 226225017

Centisome position: 86.49

GC content: 40.02

Gene sequence:

>1017_bases
ATGTCCAAATTATTTTCAGAATATAAATTAAAAGATGTAACGCTAAAAAACAGAATTGTTATGTCACCAATGTGTATGTA
TTCAGTAGAAAATAAAGACGGGATTGCAACAGACTTTCATTTTGCACACTATGTCTCGAGAGCTGCTGGTGGTACAGGGC
TTGTCATTTTAGAAGCTACGGCTGTTCAAGAAGTAGGCCGGATTTCTGAATTTGATTTAGGACTATGGAATGACGAGCAA
GTTCCAGCTTTGAAAAAATTAGTAGATGGACTTCATTACCACGGAGCAAAAGCAGGAATTCAATTAGCACACGCTGGAAG
AAAAGCTGTTTTACCAGGTGAAATAGTGGCTCCTTCTGCTATTGCTTTTGATGAAAAATCAGACAAACCAGTAGAGCTTA
CGAAAGAAGCAATTAAAGAGGTTGTGGCTGATTTTAAACGTGCCGCTTATCGTGCAAAAGAAGCGGGATTTGATGTTATT
GAGATTCATGCTGCTCATGGTTATTTAATTCACCAATTCTTATCACCAATTACGAATCGGCGCGAAGATAATTACGGTGG
ACCTGCTGGCAATCGATATAAAATTTTAAGTGATATTATTAAAGCAGTGAAGGAAGTATGGGACGGCCCGATTATCGTTC
GAGTATCCGCTACTGACTATGCACATGGCGGTTTGCAACTAGAAGATCATATTCCTTTTGCAAAATGGATGAAAGCAGAT
GGCGTGGAACTAATTGATGTGAGTACAGGTGGTTTAGTTAATGTGGCTCCACCAGTTTTCCCAGGTTATCAAGTGCCATT
CGCAGATGAAATTCGTCGCGGGGCGGGTATTGCGACTGGAGCGCTTGGACTTATTACACGAGGCGAACAAGCGGAAGAAA
TTTTATGCAATGAACGAGCGGATTTAATTATTGTTGGTCGTGAATTATTACGAAATCCTTATTTTGCTAAAGATGCAGCC
AAACAATTAGGCGAAACAATTGAAGGACCAAAACAATATTCACGCGCGTGGAAATAA

Upstream 100 bases:

>100_bases
TAGATCAGATTGCAGAGCGCCTTAGAAGCGGGGAAACACCAGCACAAGTAGGACTGAAAATCAGTAATAGTTAATTAAAA
AGAAAAGGATGCGAATTAGA

Downstream 100 bases:

>100_bases
ATAAAAAGCTGGAAGTTCAGAGTTTTTGAACTTCCAGCTTTTTTAGTAGAAATTAATTAATAGTTACATTCCAATCATAA
GAGCATACGCCTCCAGTGGC

Product: NADPH dehydrogenase NamA

Products: NA

Alternate protein names: Xenobiotic reductase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSKLFSEYKLKDVTLKNRIVMSPMCMYSVENKDGIATDFHFAHYVSRAAGGTGLVILEATAVQEVGRISEFDLGLWNDEQ
VPALKKLVDGLHYHGAKAGIQLAHAGRKAVLPGEIVAPSAIAFDEKSDKPVELTKEAIKEVVADFKRAAYRAKEAGFDVI
EIHAAHGYLIHQFLSPITNRREDNYGGPAGNRYKILSDIIKAVKEVWDGPIIVRVSATDYAHGGLQLEDHIPFAKWMKAD
GVELIDVSTGGLVNVAPPVFPGYQVPFADEIRRGAGIATGALGLITRGEQAEEILCNERADLIIVGRELLRNPYFAKDAA
KQLGETIEGPKQYSRAWK

Sequences:

>Translated_338_residues
MSKLFSEYKLKDVTLKNRIVMSPMCMYSVENKDGIATDFHFAHYVSRAAGGTGLVILEATAVQEVGRISEFDLGLWNDEQ
VPALKKLVDGLHYHGAKAGIQLAHAGRKAVLPGEIVAPSAIAFDEKSDKPVELTKEAIKEVVADFKRAAYRAKEAGFDVI
EIHAAHGYLIHQFLSPITNRREDNYGGPAGNRYKILSDIIKAVKEVWDGPIIVRVSATDYAHGGLQLEDHIPFAKWMKAD
GVELIDVSTGGLVNVAPPVFPGYQVPFADEIRRGAGIATGALGLITRGEQAEEILCNERADLIIVGRELLRNPYFAKDAA
KQLGETIEGPKQYSRAWK
>Mature_337_residues
SKLFSEYKLKDVTLKNRIVMSPMCMYSVENKDGIATDFHFAHYVSRAAGGTGLVILEATAVQEVGRISEFDLGLWNDEQV
PALKKLVDGLHYHGAKAGIQLAHAGRKAVLPGEIVAPSAIAFDEKSDKPVELTKEAIKEVVADFKRAAYRAKEAGFDVIE
IHAAHGYLIHQFLSPITNRREDNYGGPAGNRYKILSDIIKAVKEVWDGPIIVRVSATDYAHGGLQLEDHIPFAKWMKADG
VELIDVSTGGLVNVAPPVFPGYQVPFADEIRRGAGIATGALGLITRGEQAEEILCNERADLIIVGRELLRNPYFAKDAAK
QLGETIEGPKQYSRAWK

Specific function: Catalyzes the reduction of the double bond of an array of alpha, beta-unsaturated aldehydes and ketones. It also reduces the nitro group of nitroester and nitroaromatic compounds. It could have a role in detoxification processes [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. NamA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=342, Percent_Identity=33.0409356725146, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789463, Length=314, Percent_Identity=29.6178343949045, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17566914, Length=260, Percent_Identity=31.5384615384615, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17565138, Length=180, Percent_Identity=34.4444444444444, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=177, Percent_Identity=35.5932203389831, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=260, Percent_Identity=29.2307692307692, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=171, Percent_Identity=36.2573099415205, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564188, Length=169, Percent_Identity=36.094674556213, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540738, Length=263, Percent_Identity=27.3764258555133, Blast_Score=89, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6325086, Length=223, Percent_Identity=29.1479820627803, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321973, Length=220, Percent_Identity=28.6363636363636, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: =1.6.99.1 [H]

Molecular weight: Translated: 37050; Mature: 36919

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLFSEYKLKDVTLKNRIVMSPMCMYSVENKDGIATDFHFAHYVSRAAGGTGLVILEAT
CCCHHHHHHHHHEEHHCEEEECCHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHH
AVQEVGRISEFDLGLWNDEQVPALKKLVDGLHYHGAKAGIQLAHAGRKAVLPGEIVAPSA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEHECCCCCCCCCCCCCCCCE
IAFDEKSDKPVELTKEAIKEVVADFKRAAYRAKEAGFDVIEIHAAHGYLIHQFLSPITNR
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCC
REDNYGGPAGNRYKILSDIIKAVKEVWDGPIIVRVSATDYAHGGLQLEDHIPFAKWMKAD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHCCC
GVELIDVSTGGLVNVAPPVFPGYQVPFADEIRRGAGIATGALGLITRGEQAEEILCNERA
CCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHCCCC
DLIIVGRELLRNPYFAKDAAKQLGETIEGPKQYSRAWK
CEEEECHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
SKLFSEYKLKDVTLKNRIVMSPMCMYSVENKDGIATDFHFAHYVSRAAGGTGLVILEAT
CCHHHHHHHHHEEHHCEEEECCHHEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEHH
AVQEVGRISEFDLGLWNDEQVPALKKLVDGLHYHGAKAGIQLAHAGRKAVLPGEIVAPSA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHCEEHECCCCCCCCCCCCCCCCE
IAFDEKSDKPVELTKEAIKEVVADFKRAAYRAKEAGFDVIEIHAAHGYLIHQFLSPITNR
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHCC
REDNYGGPAGNRYKILSDIIKAVKEVWDGPIIVRVSATDYAHGGLQLEDHIPFAKWMKAD
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCHHHHHCCC
GVELIDVSTGGLVNVAPPVFPGYQVPFADEIRRGAGIATGALGLITRGEQAEEILCNERA
CCEEEEECCCCEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCCHHHHHHHCCCC
DLIIVGRELLRNPYFAKDAAKQLGETIEGPKQYSRAWK
CEEEECHHHHCCCCCHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]