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The map label for this gene is gap

Identifier: 226225007

GI number: 226225007

Start: 2504041

End: 2505051

Strand: Reverse

Name: gap

Synonym: Lm4b_02428

Alternate gene names: 226225007

Gene position: 2505051-2504041 (Counterclockwise)

Preceding gene: 226225008

Following gene: 226225006

Centisome position: 86.0

GC content: 38.97

Gene sequence:

>1011_bases
ATGACAGTTAAAGTTGGTATTAATGGTTTTGGACGTATCGGACGTCTAGCATTCCGTCGTATTCAAAATGTGGAAGGAAT
TGAAGTTGTTGCAATCAATGACTTAACAGACGCTAAAATGTTAGCTCACCTGTTAAAATATGATACAACTCAAGGCCGTT
TTGACGGTGAAGTAGAAGTACATGATGGTTTCTTCAACGTAAACGGTAAAGAAGTTAAAGTATTAGCTAACCGTAACCCA
GAAGAACTTCCATGGGGTGACCTAGGAGTAGACATCGTTTTAGAATGTACTGGTTTCTTCACAGCGCAAGACAAAGCTGA
ATTACACATTAAAGCTGGCGCTAAAAAAGTTGTTATCTCCGCTCCAGCAACTGGCGACATGAAAACAATCGTTTACAATG
TAAACCATGAAACATTAGACGGAACTGAAACAGTTATCTCTGGCGCAAGCTGTACTACTAACTGTTTAGCTCCTATGGCT
AAAGTTTTAGAAGACAAATTTGGTGTTGTTGAAGGTCTAATGACTACAATTCACGCTTACACTGGTGACCAAAATACATT
AGATGCTCCACATCCAAAAGGTGACTTCCGTCGTGCACGTGCTGCTGCCGAAAATATTATCCCTAATACAACTGGTGCTG
CTAAAGCTATCGGTGAAGTATTACCAACACTTAAAGGTAAATTAGACGGAGCTGCTCAACGTGTTCCAGTTCCAACTGGT
TCCCTTACTGAATTAGTAACAGTTCTTGACAAAAAAGTTACTGTTGATGAAGTAAATGCAGCTATGGAAGCAGCTTCTGA
TCCAGAAACATTCGGTTACACTAGTGACCAAGTTGTTTCTTCTGACATCAAAGGTATGACTTTCGGTTCATTATTTGACG
AAACTCAAACAAAAGTTCTTACAGTTGGCGATCAACAATTAGTTAAAACTGTAGCTTGGTACGATAACGAAATGAGCTAC
ACTGCTCAATTAGTACGTACTTTAGAATACTTTGCAAAAATCGCTAAATAA

Upstream 100 bases:

>100_bases
CGATTTTAATCACGGATGAAGGAGCAGCAAAATCGCTTTTAAAAGGGAGTAATACCCTTTTGAAATAAAAAAACACTATT
TTCAAGGAGGAAATTGACTT

Downstream 100 bases:

>100_bases
TTAGCAGATTAATTTTACTGAAGTGCTAGTCACATCAGCAATAACAAGAATAAGCGGAGACACTATTGTTTCCGCTTATT
TTCTGAAATAACGGGGGCGA

Product: glyceraldehyde 3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEVHDGFFNVNGKEVKVLANRNP
EELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVISAPATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMA
KVLEDKFGVVEGLMTTIHAYTGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG
SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVLTVGDQQLVKTVAWYDNEMSY
TAQLVRTLEYFAKIAK

Sequences:

>Translated_336_residues
MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEVHDGFFNVNGKEVKVLANRNP
EELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVISAPATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMA
KVLEDKFGVVEGLMTTIHAYTGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG
SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVLTVGDQQLVKTVAWYDNEMSY
TAQLVRTLEYFAKIAK
>Mature_335_residues
TVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEVHDGFFNVNGKEVKVLANRNPE
ELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVISAPATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAK
VLEDKFGVVEGLMTTIHAYTGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTGS
LTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVLTVGDQQLVKTVAWYDNEMSYT
AQLVRTLEYFAKIAK

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7669492, Length=334, Percent_Identity=44.9101796407186, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI7657116, Length=332, Percent_Identity=43.9759036144578, Blast_Score=274, Evalue=8e-74,
Organism=Escherichia coli, GI1788079, Length=336, Percent_Identity=49.702380952381, Blast_Score=330, Evalue=7e-92,
Organism=Escherichia coli, GI1789295, Length=342, Percent_Identity=40.6432748538012, Blast_Score=250, Evalue=9e-68,
Organism=Caenorhabditis elegans, GI32566163, Length=339, Percent_Identity=46.6076696165192, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17568413, Length=339, Percent_Identity=46.6076696165192, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17534679, Length=339, Percent_Identity=45.7227138643068, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17534677, Length=339, Percent_Identity=45.4277286135693, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6322409, Length=335, Percent_Identity=45.6716417910448, Blast_Score=292, Evalue=5e-80,
Organism=Saccharomyces cerevisiae, GI6321631, Length=335, Percent_Identity=46.5671641791045, Blast_Score=288, Evalue=8e-79,
Organism=Saccharomyces cerevisiae, GI6322468, Length=335, Percent_Identity=45.9701492537313, Blast_Score=286, Evalue=4e-78,
Organism=Drosophila melanogaster, GI85725000, Length=331, Percent_Identity=46.5256797583082, Blast_Score=287, Evalue=6e-78,
Organism=Drosophila melanogaster, GI22023983, Length=331, Percent_Identity=46.5256797583082, Blast_Score=287, Evalue=6e-78,
Organism=Drosophila melanogaster, GI17933600, Length=332, Percent_Identity=46.0843373493976, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI18110149, Length=332, Percent_Identity=46.0843373493976, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI19922412, Length=330, Percent_Identity=44.2424242424242, Blast_Score=279, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 36273; Mature: 36141

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV
CEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEE
HDGFFNVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS
ECCEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEECCCEEEEEE
APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY
CCCCCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCEEE
TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK
EECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TVKVGINGFGRIGRLAFRRIQNVEGIEVVAINDLTDAKMLAHLLKYDTTQGRFDGEVEV
EEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCEEEE
HDGFFNVNGKEVKVLANRNPEELPWGDLGVDIVLECTGFFTAQDKAELHIKAGAKKVVIS
ECCEEECCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCEEECCCCEEEEECCCEEEEEE
APATGDMKTIVYNVNHETLDGTETVISGASCTTNCLAPMAKVLEDKFGVVEGLMTTIHAY
CCCCCCCEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGDQNTLDAPHPKGDFRRARAAAENIIPNTTGAAKAIGEVLPTLKGKLDGAAQRVPVPTG
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SLTELVTVLDKKVTVDEVNAAMEAASDPETFGYTSDQVVSSDIKGMTFGSLFDETQTKVL
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCHHHHHHCCCCEEE
TVGDQQLVKTVAWYDNEMSYTAQLVRTLEYFAKIAK
EECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463150; 11466286 [H]