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The map label for this gene is gcvH [H]

Identifier: 226224972

GI number: 226224972

Start: 2466343

End: 2466720

Strand: Reverse

Name: gcvH [H]

Synonym: Lm4b_02393

Alternate gene names: 226224972

Gene position: 2466720-2466343 (Counterclockwise)

Preceding gene: 226224973

Following gene: 226224971

Centisome position: 84.69

GC content: 36.51

Gene sequence:

>378_bases
ATGAGTTTACCAAAAGACTTATTGTACACAGAAGAACATGAATGGGTGAAAGCAGACGAGGGTAGTTATGTTATTGGGAT
TACGGACTTTGCTCAAGATCAATTAGGGGATATCGTATTTGTTGAATTGCCAGAGGTTGGCGACACTGTAGCTAAAGGCG
ACTCTATTGGTAGCATTGAGTCTGTAAAAACAGTATCTGATTTTTATGCACCAGTAACTGGTAAAGTAGTTGCTGTTAAC
GAAACATTAGAAGATGAACCAGAATTAATTAATAGCAATCCATACGATACAGGCTGGATTTTAAAACTTGAAGAAGTAGA
AGAAGCGGATGTAAAGGCTCTTTTATCAAGTGACGATTACGAAAAAGTATTAGATTAA

Upstream 100 bases:

>100_bases
AACGGCAAAGAAGTGACGCTTGGTTTCAAAGAAGAAGAATTTGAAGCAACTTGGAAATAGTCAATATAGATAATTGCACG
AAAAAGGAGGAGTTTTGAAA

Downstream 100 bases:

>100_bases
TTTTTAGGAGGCCGGCTTGGGATAACCGAAGCTGGTCTTTTTTGATAGGAGGCGTTTTTCCTGGAAATTTGGGAAAAAGA
ACGTTTGCGGACGGCGCAAA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MSLPKDLLYTEEHEWVKADEGSYVIGITDFAQDQLGDIVFVELPEVGDTVAKGDSIGSIESVKTVSDFYAPVTGKVVAVN
ETLEDEPELINSNPYDTGWILKLEEVEEADVKALLSSDDYEKVLD

Sequences:

>Translated_125_residues
MSLPKDLLYTEEHEWVKADEGSYVIGITDFAQDQLGDIVFVELPEVGDTVAKGDSIGSIESVKTVSDFYAPVTGKVVAVN
ETLEDEPELINSNPYDTGWILKLEEVEEADVKALLSSDDYEKVLD
>Mature_124_residues
SLPKDLLYTEEHEWVKADEGSYVIGITDFAQDQLGDIVFVELPEVGDTVAKGDSIGSIESVKTVSDFYAPVTGKVVAVNE
TLEDEPELINSNPYDTGWILKLEEVEEADVKALLSSDDYEKVLD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=41.025641025641, Blast_Score=113, Evalue=5e-26,
Organism=Homo sapiens, GI89057342, Length=114, Percent_Identity=41.2280701754386, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789271, Length=125, Percent_Identity=50.4, Blast_Score=137, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=49.5652173913044, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=48.695652173913, Blast_Score=122, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=42.4, Blast_Score=108, Evalue=2e-25,
Organism=Drosophila melanogaster, GI17865652, Length=124, Percent_Identity=41.9354838709677, Blast_Score=113, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13785; Mature: 13654

Theoretical pI: Translated: 3.67; Mature: 3.67

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPKDLLYTEEHEWVKADEGSYVIGITDFAQDQLGDIVFVELPEVGDTVAKGDSIGSIE
CCCCCCCEECCCCCEEEECCCCEEEEECCCCHHCCCCEEEEECCCCCCHHHCCCCCCCHH
SVKTVSDFYAPVTGKVVAVNETLEDEPELINSNPYDTGWILKLEEVEEADVKALLSSDDY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCH
EKVLD
HHHCC
>Mature Secondary Structure 
SLPKDLLYTEEHEWVKADEGSYVIGITDFAQDQLGDIVFVELPEVGDTVAKGDSIGSIE
CCCCCCEECCCCCEEEECCCCEEEEECCCCHHCCCCEEEEECCCCCCHHHCCCCCCCHH
SVKTVSDFYAPVTGKVVAVNETLEDEPELINSNPYDTGWILKLEEVEEADVKALLSSDDY
HHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCH
EKVLD
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]