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The map label for this gene is ytzG [H]

Identifier: 226224887

GI number: 226224887

Start: 2375294

End: 2375995

Strand: Reverse

Name: ytzG [H]

Synonym: Lm4b_02306

Alternate gene names: 226224887

Gene position: 2375995-2375294 (Counterclockwise)

Preceding gene: 226224888

Following gene: 226224886

Centisome position: 81.57

GC content: 40.88

Gene sequence:

>702_bases
ATGCGCTTAGATAAATTATTGTCCCATACAGGTTTCGGGAGTCGAAAAGAAGTGAAACCTTTGCTTAAATCTGGGGCAGT
TGTCGTCAATGGCACGATTCAGAAAGATAGCAAAACCCAAGTAAACCCTGATAAAGACCAAGTAACTGTCCATGGAACAC
CAGTCGTTTACCAAGAATTCGTCTATTTTATGCTACATAAACCACAAAATGTCGTGAGCGCAACCGAGGATAATGTGTCA
GAAACCGTCATCGATTTGCTTGCGCAAGAAGACACGCTCACTGATCCATTCCCAGTCGGCCGCTTAGACAAAGATACAGA
AGGCTTGCTAATTATAACGAACGATGGCACACTCGCGCACAATTTGCTCTCACCTAAAAAACATATAGATAAAACATACT
ACGCTAAAATTGATGGTGATGTTACTCTAGCTGATGTAGAAGCTTTTGCAGCTGGAATTGAACTCGACGATGGATACACT
TGCAAACCAGCACGTTTAGAAATTATTACACCAAACGAAATAAACGTCACTATCCAAGAAGGGAAATTCCATCAAGTAAA
AAGAATGTTCGCCGCACGTGGTAAGAAAGTCAGCTATTTAAAACGAATTTCGATGGGGAATTTACAATTAGACGAGTCAC
TTGGACTCGGCGAATACCGCCCTTTAACTGAAAGTGAACTGGCTATTCTTCAAAATAAATGA

Upstream 100 bases:

>100_bases
AATAAACAAGTTGTTGAAACTATATAATAGAAAGAAAACGGAGCACCTTTGAGGAAGCTTCGTTTTTTTGTTACAATAAA
AGAAAAAAAGGAGCGAAACC

Downstream 100 bases:

>100_bases
ACCTAAACGAAAATTTCCTTCCTAAAAAGAAGCGGATTTTCGTTTTTTAGTTTGCAAAAGAAAGCATTTACATATATAAT
AAACATGTGTTTGAAAATAA

Product: 16S pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MRLDKLLSHTGFGSRKEVKPLLKSGAVVVNGTIQKDSKTQVNPDKDQVTVHGTPVVYQEFVYFMLHKPQNVVSATEDNVS
ETVIDLLAQEDTLTDPFPVGRLDKDTEGLLIITNDGTLAHNLLSPKKHIDKTYYAKIDGDVTLADVEAFAAGIELDDGYT
CKPARLEIITPNEINVTIQEGKFHQVKRMFAARGKKVSYLKRISMGNLQLDESLGLGEYRPLTESELAILQNK

Sequences:

>Translated_233_residues
MRLDKLLSHTGFGSRKEVKPLLKSGAVVVNGTIQKDSKTQVNPDKDQVTVHGTPVVYQEFVYFMLHKPQNVVSATEDNVS
ETVIDLLAQEDTLTDPFPVGRLDKDTEGLLIITNDGTLAHNLLSPKKHIDKTYYAKIDGDVTLADVEAFAAGIELDDGYT
CKPARLEIITPNEINVTIQEGKFHQVKRMFAARGKKVSYLKRISMGNLQLDESLGLGEYRPLTESELAILQNK
>Mature_233_residues
MRLDKLLSHTGFGSRKEVKPLLKSGAVVVNGTIQKDSKTQVNPDKDQVTVHGTPVVYQEFVYFMLHKPQNVVSATEDNVS
ETVIDLLAQEDTLTDPFPVGRLDKDTEGLLIITNDGTLAHNLLSPKKHIDKTYYAKIDGDVTLADVEAFAAGIELDDGYT
CKPARLEIITPNEINVTIQEGKFHQVKRMFAARGKKVSYLKRISMGNLQLDESLGLGEYRPLTESELAILQNK

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=40.8695652173913, Blast_Score=160, Evalue=6e-41,
Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=31.7391304347826, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=30.2904564315353, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=35.6164383561644, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25867; Mature: 25867

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDKLLSHTGFGSRKEVKPLLKSGAVVVNGTIQKDSKTQVNPDKDQVTVHGTPVVYQEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHH
VYFMLHKPQNVVSATEDNVSETVIDLLAQEDTLTDPFPVGRLDKDTEGLLIITNDGTLAH
HHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
NLLSPKKHIDKTYYAKIDGDVTLADVEAFAAGIELDDGYTCKPARLEIITPNEINVTIQE
HHCCCHHHCCCEEEEEECCCEEEEEHHHHHHCEECCCCCCCCCCEEEEECCCCEEEEEEC
GKFHQVKRMFAARGKKVSYLKRISMGNLQLDESLGLGEYRPLTESELAILQNK
CCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHEEECCC
>Mature Secondary Structure
MRLDKLLSHTGFGSRKEVKPLLKSGAVVVNGTIQKDSKTQVNPDKDQVTVHGTPVVYQEF
CCHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCHHHHHH
VYFMLHKPQNVVSATEDNVSETVIDLLAQEDTLTDPFPVGRLDKDTEGLLIITNDGTLAH
HHHHHCCCCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHH
NLLSPKKHIDKTYYAKIDGDVTLADVEAFAAGIELDDGYTCKPARLEIITPNEINVTIQE
HHCCCHHHCCCEEEEEECCCEEEEEHHHHHHCEECCCCCCCCCCEEEEECCCCEEEEEEC
GKFHQVKRMFAARGKKVSYLKRISMGNLQLDESLGLGEYRPLTESELAILQNK
CCHHHHHHHHHHCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]