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The map label for this gene is trpS

Identifier: 226224806

GI number: 226224806

Start: 2293464

End: 2294459

Strand: Direct

Name: trpS

Synonym: Lm4b_02225

Alternate gene names: 226224806

Gene position: 2293464-2294459 (Clockwise)

Preceding gene: 226224805

Following gene: 226224812

Centisome position: 78.74

GC content: 36.65

Gene sequence:

>996_bases
ATGAAAAAAGTAATATTTTCCGGAATTCAACCTAGTGGACAATTAACTTTAGGAAATTATATTGGAGCTTTGAAACAGTT
TGGTCAGTTTCAAGATGAATATGAATGTTTTTATTGTATCGTTGACGAACACGCTATTACGGTTCCACAAGATAGATTAA
AGCTTCGTGAGCAAACAAGAAGTTTAGCAGCTCTTTATTTAGCAGTTGGACTGGATCCTGAAAAAGCGACTCTTTTTATT
CAATCTGAAGTTGCAGCACATGCTCAAGCAGCATGGATTTTACAATGTAACGTATATATTGGCGAATTAGAACGTATGAC
GCAGTTCAAAGATAAATCAGATGGGAAAGCAGGTGTCAGTGCTGGCCTTTTAACTTACCCACCATTGATGGCAGCTGATA
TTTTACTTTATCAAACCGATTTAGTTCCTGTTGGCGAGGATCAGAAGCAACATATCGAATTAACTCGCGACTTAGCAGAA
CGATTTAATAAAAAGCACGCAGATATTTTCACGATGCCAGAAGTATTCATTCCTAAACAAGGCGCACGAGTAATGTCCTT
ACAAGATCCAACGAAAAAAATGAGTAAATCTGATGCCAATTTGAAAAATGCTATCTTTTTACTTGACCCACCAGCTACCA
TTAGAAAAAAAATCAAAAGCGCTGTAACCGATTCTAGTGGTATTATTGAATATAATAAAGAAGAAAAACCAGGTGTGTCT
AACTTACTAACTATCTATTCTGTTATCACTGGCGAAACAATTTCTACTATAGAAGAAAAATATGTTGGAAAAGGTTATGG
CGATTTTAAAACAGATTTAGCTGAATTAGTTGTTGCTGAACTTGAGCCAATTCAAGAAAGGTACTATGCTTATCTAAAAT
CTGAGGAGCTGGACACTATATTAGACGCAGGAGCAGAAAAAGCTGCTCGCGTGGCTAATAAAACATTGAAGAAAATGGAA
AATGGCGTTGGGCTTGGCCGCAAACGCAGAAGATAA

Upstream 100 bases:

>100_bases
TTAGGGTGGCACCGCGGATAACCGTTCGTCCCTAACATTTATATGTTGGGATGGACGGTTTTTTATTTTCGCAATCAGTG
AAAAAATTGGAGGAATAAAC

Downstream 100 bases:

>100_bases
TATAAAAAAGCCAATGACTAGTTGATCTAAGTCATTGGTTTTTTTTATTTAAACATTAACCTACTTTAATTTCCACATCG
ATGTTACCACGAGTTGCTTT

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEYECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLDPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTDLVPVGEDQKQHIELTRDLAE
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVITGETISTIEEKYVGKGYGDFKTDLAELVVAELEPIQERYYAYLKSEELDTILDAGAEKAARVANKTLKKME
NGVGLGRKRRR

Sequences:

>Translated_331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEYECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLDPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTDLVPVGEDQKQHIELTRDLAE
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVITGETISTIEEKYVGKGYGDFKTDLAELVVAELEPIQERYYAYLKSEELDTILDAGAEKAARVANKTLKKME
NGVGLGRKRRR
>Mature_331_residues
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEYECFYCIVDEHAITVPQDRLKLREQTRSLAALYLAVGLDPEKATLFI
QSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVSAGLLTYPPLMAADILLYQTDLVPVGEDQKQHIELTRDLAE
RFNKKHADIFTMPEVFIPKQGARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
NLLTIYSVITGETISTIEEKYVGKGYGDFKTDLAELVVAELEPIQERYYAYLKSEELDTILDAGAEKAARVANKTLKKME
NGVGLGRKRRR

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=327, Percent_Identity=40.6727828746177, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI41352700, Length=175, Percent_Identity=52.5714285714286, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1789786, Length=328, Percent_Identity=56.0975609756098, Blast_Score=370, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71982800, Length=330, Percent_Identity=36.6666666666667, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI71982793, Length=330, Percent_Identity=36.6666666666667, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6320474, Length=348, Percent_Identity=40.5172413793103, Blast_Score=223, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24666151, Length=325, Percent_Identity=43.6923076923077, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 36963; Mature: 36963

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEYECFYCIVDEHAITVPQDRLKLREQTR
CCCCEECCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCHHHHHHHHHHH
SLAALYLAVGLDPEKATLFIQSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVS
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
AGLLTYPPLMAADILLYQTDLVPVGEDQKQHIELTRDLAERFNKKHADIFTMPEVFIPKQ
CCCHHCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCC
GARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
CCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSVITGETISTIEEKYVGKGYGDFKTDLAELVVAELEPIQERYYAYLKSEELDTI
HHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAGAEKAARVANKTLKKMENGVGLGRKRRR
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKKVIFSGIQPSGQLTLGNYIGALKQFGQFQDEYECFYCIVDEHAITVPQDRLKLREQTR
CCCCEECCCCCCCCEEHHHHHHHHHHHHCCCCCCEEEEEEEECCEEECCHHHHHHHHHHH
SLAALYLAVGLDPEKATLFIQSEVAAHAQAAWILQCNVYIGELERMTQFKDKSDGKAGVS
HHHHHHHHHCCCCCCEEEEEEHHHHHHHHEEEEEEECHHHHHHHHHHHHCCCCCCCCCCC
AGLLTYPPLMAADILLYQTDLVPVGEDQKQHIELTRDLAERFNKKHADIFTMPEVFIPKQ
CCCHHCCHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCHHCCCCC
GARVMSLQDPTKKMSKSDANLKNAIFLLDPPATIRKKIKSAVTDSSGIIEYNKEEKPGVS
CCEEEECCCHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEECCCCCCCHH
NLLTIYSVITGETISTIEEKYVGKGYGDFKTDLAELVVAELEPIQERYYAYLKSEELDTI
HHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAGAEKAARVANKTLKKMENGVGLGRKRRR
HHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]