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The map label for this gene is gloA [H]

Identifier: 226224775

GI number: 226224775

Start: 2251489

End: 2251878

Strand: Direct

Name: gloA [H]

Synonym: Lm4b_02193

Alternate gene names: 226224775

Gene position: 2251489-2251878 (Clockwise)

Preceding gene: 226224763

Following gene: 226224776

Centisome position: 77.3

GC content: 36.92

Gene sequence:

>390_bases
ATGACTGCAAAAATGTTACATACATGTATCCGCGTAAAAAACCTAACAGAATCGATTACTTTTTACGAAAAAGCATTAGG
ACTTAAAGAAGTTCGCCGCAAGGACTTCCCGGATTTCGAATTCACATTAGTATATATGGCGTTTGAAGAAGGCGGTTTTG
AACTAGAATTAACGTACAACTACGACCAAAAAGAAGCCTACGATTTAGGAAATGGTTATGGTCACTTAGCAGTTGGTGTT
CCGGACGTACATGCTTTATTAAAAGAACACCAAGAAGCTGGTTTTACTGTGACAGATTTAAAAGGACTACCTGGCGAAGA
TCCATTCTATTATTTCTTAACAGATCCAGATGGTTATAAAACAGAAATCATTCAAGATGGCGCTTTATAA

Upstream 100 bases:

>100_bases
TATACCTAAAAAGCCACCTTACTTCCATCAATATTCCTTGTTTGTATTCACTTCCGAGATATACTACAATGAAGAAAGAA
GTTTAAAAGGAGGAATAAAA

Downstream 100 bases:

>100_bases
TTAGAAGAACTGGAGAAGCTTAATGGGAAAATATTTTATTATAATTTTGATCGCACTAGCAATTAATGGAATCAGTATTT
TATTCCATAATGATATAGCA

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MTAKMLHTCIRVKNLTESITFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGV
PDVHALLKEHQEAGFTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL

Sequences:

>Translated_129_residues
MTAKMLHTCIRVKNLTESITFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGV
PDVHALLKEHQEAGFTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL
>Mature_128_residues
TAKMLHTCIRVKNLTESITFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYNYDQKEAYDLGNGYGHLAVGVP
DVHALLKEHQEAGFTVTDLKGLPGEDPFYYFLTDPDGYKTEIIQDGAL

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=156, Percent_Identity=29.4871794871795, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787940, Length=126, Percent_Identity=35.7142857142857, Blast_Score=82, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=151, Percent_Identity=31.7880794701987, Blast_Score=64, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 14716; Mature: 14585

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAKMLHTCIRVKNLTESITFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYN
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEC
YDQKEAYDLGNGYGHLAVGVPDVHALLKEHQEAGFTVTDLKGLPGEDPFYYFLTDPDGYK
CCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCE
TEIIQDGAL
EEEEECCCC
>Mature Secondary Structure 
TAKMLHTCIRVKNLTESITFYEKALGLKEVRRKDFPDFEFTLVYMAFEEGGFELELTYN
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCEEEEEEEEEECCCEEEEEEEC
YDQKEAYDLGNGYGHLAVGVPDVHALLKEHQEAGFTVTDLKGLPGEDPFYYFLTDPDGYK
CCCHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCE
TEIIQDGAL
EEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]