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The map label for this gene is nadB

Identifier: 226224620

GI number: 226224620

Start: 2091347

End: 2092801

Strand: Direct

Name: nadB

Synonym: Lm4b_02035

Alternate gene names: 226224620

Gene position: 2091347-2092801 (Clockwise)

Preceding gene: 226224612

Following gene: 226224621

Centisome position: 71.8

GC content: 43.16

Gene sequence:

>1455_bases
ATGACAAAAGAACGAGTGATTATCATTGGGAGCGGTATCGCAGGTTGCACTGCTGCCTTCAGATTAATGCACGACTATGA
TGTGACAATAATCACAAAAGGTCTCAAAGAAGAAAGTAATTCGATGCTCGCTCAAGGCGGTGTCGCAGCAGCTGTGTCCA
AAAACGATACCCCGAAAAAGCATTTTAGCGATACCTTCCAAGCCGGTTGTTTTCATAATAAAGTTCTCGCAGTTAATCAA
CTCGTTACCAACGGTCCAATCGTTATCCAAAAATTAATCGCAGAAGGGATGACTTTTGACGAACAAAATGGTGAACTTTC
GCTTGGTTTAGAAGGCGCACACCAATTGCCACGAATTTTACATACTGGCGGTGATCAAACCGGCAAATTTCTTACTACTT
TTTTACAAGAAAAATTAACAAACATTCACTGGCAAGAGCAAAAAATGGCTATCGAAATTATAAAACAGAACGATTCGGCT
ATCGGCGTGCATTGTTTAGATAAAGAAAATCGACTACATACTTATTACGGAGAACATATTATTTTAGCAAGCGGCGGACT
TGGACAACTTTTTCCAGTTACGACGAATGCGGCGACAATTTCCGGTGATGGCTTAGCGCTAGCTTACCGGGCTGGTGCGA
AACTAACCGACATGGAATTTATCCAATTTCATCCCACTCTTCTCTTTCTAAACGGACGTTGTCATGGGCTTATTTCAGAA
GCGGTCCGCGGTGAAGGAGCCAAACTTATTCGCGCAGATGGTTCGGCGGTAATGACTGATGTTCACCCGAGAGCTGACCT
TGCGCCGCGCGATATTGTTGCTGCGACGCTATTTGCAGAAATACAAGATGGCAATGAGGTTTTCCTTGATATCACGCCAA
TTCCTAATTTTGAAGAACGTTTTCCAGGAATTACTGCTAACTTGGATGCATATCACGTCCCGTTTCGTGAAACAAAGCGG
ATTCCGGTTCATCCTGGCGCACATTTTTTAATGGGTGGTATTCGTACTGATCTTCAAGGAAAAACGAACATCCCTGGTTT
ATATGCCATTGGTGAGGTTGCAAATGCAGGAGTGCACGGCGCTAATCGTCTCGCCAGCAATTCCCTCCTTGAAACGCTCG
TCTTTGGTGAAAAAGTGGCTGAATACATTCTCACGCAAAAAACAAACCTAATAGACCATCCAGAAATCCCTCTTTCGAAC
CAAACCCAAACGCCCCATTTACCAGATAAACAATTACTTCAAGAGAAAATCTGGGAAACGCTTGGCATCACAAGAAAACC
AGAAAAAATCACCGAATTTCTTCATTGGCTTACAGATTTCGATTACGCAAATCACACTCGAGAAACAGCGGAAATTAGCC
ACATGCTTATCACCGCAAAACTAATCGCCGAAAGCGCGCTCAAACGAACAGAAAGTCTCGGCGCACATCGAATATTAAAA
GGAGTAATAAAATGA

Upstream 100 bases:

>100_bases
GAATTTAGTTTGATTTTATGTGATTATAGATGACAAGACATCTGTATATTCATTAAGAAGTATACAATTAATCGAACCTA
CTATGCAAGAGGTGGAAACA

Downstream 100 bases:

>100_bases
ATTCCATACTTATGAATCAAGCAATTCAAGCATTTTTACTAGAAGATATCGGTCAACATGACTTAAGTGCAGAAACTGTT
TTCCCCCGTGACACAATGGG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTKERVIIIGSGIAGCTAAFRLMHDYDVTIITKGLKEESNSMLAQGGVAAAVSKNDTPKKHFSDTFQAGCFHNKVLAVNQ
LVTNGPIVIQKLIAEGMTFDEQNGELSLGLEGAHQLPRILHTGGDQTGKFLTTFLQEKLTNIHWQEQKMAIEIIKQNDSA
IGVHCLDKENRLHTYYGEHIILASGGLGQLFPVTTNAATISGDGLALAYRAGAKLTDMEFIQFHPTLLFLNGRCHGLISE
AVRGEGAKLIRADGSAVMTDVHPRADLAPRDIVAATLFAEIQDGNEVFLDITPIPNFEERFPGITANLDAYHVPFRETKR
IPVHPGAHFLMGGIRTDLQGKTNIPGLYAIGEVANAGVHGANRLASNSLLETLVFGEKVAEYILTQKTNLIDHPEIPLSN
QTQTPHLPDKQLLQEKIWETLGITRKPEKITEFLHWLTDFDYANHTRETAEISHMLITAKLIAESALKRTESLGAHRILK
GVIK

Sequences:

>Translated_484_residues
MTKERVIIIGSGIAGCTAAFRLMHDYDVTIITKGLKEESNSMLAQGGVAAAVSKNDTPKKHFSDTFQAGCFHNKVLAVNQ
LVTNGPIVIQKLIAEGMTFDEQNGELSLGLEGAHQLPRILHTGGDQTGKFLTTFLQEKLTNIHWQEQKMAIEIIKQNDSA
IGVHCLDKENRLHTYYGEHIILASGGLGQLFPVTTNAATISGDGLALAYRAGAKLTDMEFIQFHPTLLFLNGRCHGLISE
AVRGEGAKLIRADGSAVMTDVHPRADLAPRDIVAATLFAEIQDGNEVFLDITPIPNFEERFPGITANLDAYHVPFRETKR
IPVHPGAHFLMGGIRTDLQGKTNIPGLYAIGEVANAGVHGANRLASNSLLETLVFGEKVAEYILTQKTNLIDHPEIPLSN
QTQTPHLPDKQLLQEKIWETLGITRKPEKITEFLHWLTDFDYANHTRETAEISHMLITAKLIAESALKRTESLGAHRILK
GVIK
>Mature_483_residues
TKERVIIIGSGIAGCTAAFRLMHDYDVTIITKGLKEESNSMLAQGGVAAAVSKNDTPKKHFSDTFQAGCFHNKVLAVNQL
VTNGPIVIQKLIAEGMTFDEQNGELSLGLEGAHQLPRILHTGGDQTGKFLTTFLQEKLTNIHWQEQKMAIEIIKQNDSAI
GVHCLDKENRLHTYYGEHIILASGGLGQLFPVTTNAATISGDGLALAYRAGAKLTDMEFIQFHPTLLFLNGRCHGLISEA
VRGEGAKLIRADGSAVMTDVHPRADLAPRDIVAATLFAEIQDGNEVFLDITPIPNFEERFPGITANLDAYHVPFRETKRI
PVHPGAHFLMGGIRTDLQGKTNIPGLYAIGEVANAGVHGANRLASNSLLETLVFGEKVAEYILTQKTNLIDHPEIPLSNQ
TQTPHLPDKQLLQEKIWETLGITRKPEKITEFLHWLTDFDYANHTRETAEISHMLITAKLIAESALKRTESLGAHRILKG
VIK

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=394, Percent_Identity=32.48730964467, Blast_Score=170, Evalue=2e-42,
Organism=Escherichia coli, GI1788928, Length=509, Percent_Identity=34.7740667976424, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1790597, Length=510, Percent_Identity=28.8235294117647, Blast_Score=176, Evalue=3e-45,
Organism=Escherichia coli, GI1786942, Length=524, Percent_Identity=28.6259541984733, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=514, Percent_Identity=29.1828793774319, Blast_Score=184, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17550100, Length=520, Percent_Identity=29.4230769230769, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI71986328, Length=439, Percent_Identity=26.4236902050114, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322416, Length=521, Percent_Identity=29.7504798464491, Blast_Score=178, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6322701, Length=524, Percent_Identity=29.5801526717557, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6320788, Length=355, Percent_Identity=24.7887323943662, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322511, Length=349, Percent_Identity=25.5014326647564, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17137288, Length=525, Percent_Identity=30.0952380952381, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655642, Length=525, Percent_Identity=30.0952380952381, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655647, Length=525, Percent_Identity=30.0952380952381, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24663005, Length=516, Percent_Identity=28.4883720930233, Blast_Score=159, Evalue=3e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 53114; Mature: 52982

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKERVIIIGSGIAGCTAAFRLMHDYDVTIITKGLKEESNSMLAQGGVAAAVSKNDTPKK
CCCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHCCCEEEEECCCCCCHH
HFSDTFQAGCFHNKVLAVNQLVTNGPIVIQKLIAEGMTFDEQNGELSLGLEGAHQLPRIL
HHHHHHHCCCCCCHHEEEHHHHCCCHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHH
HTGGDQTGKFLTTFLQEKLTNIHWQEQKMAIEIIKQNDSAIGVHCLDKENRLHTYYGEHI
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHEECCCCEEEEEEECCCCCEEEEECCEE
ILASGGLGQLFPVTTNAATISGDGLALAYRAGAKLTDMEFIQFHPTLLFLNGRCHGLISE
EEECCCCCCEEEEECCCEEECCCCEEEEEECCCCCCCCHHEEECCEEEEECCCCHHHHHH
AVRGEGAKLIRADGSAVMTDVHPRADLAPRDIVAATLFAEIQDGNEVFLDITPIPNFEER
HHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
FPGITANLDAYHVPFRETKRIPVHPGAHFLMGGIRTDLQGKTNIPGLYAIGEVANAGVHG
CCCCEECCCEEECCHHHCCCCCCCCCHHHHHCCHHCCCCCCCCCCCEEEHHHHHCCCCCC
ANRLASNSLLETLVFGEKVAEYILTQKTNLIDHPEIPLSNQTQTPHLPDKQLLQEKIWET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LGITRKPEKITEFLHWLTDFDYANHTRETAEISHMLITAKLIAESALKRTESLGAHRILK
HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIK
HHCC
>Mature Secondary Structure 
TKERVIIIGSGIAGCTAAFRLMHDYDVTIITKGLKEESNSMLAQGGVAAAVSKNDTPKK
CCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHHHCCHHHCCCEEEEECCCCCCHH
HFSDTFQAGCFHNKVLAVNQLVTNGPIVIQKLIAEGMTFDEQNGELSLGLEGAHQLPRIL
HHHHHHHCCCCCCHHEEEHHHHCCCHHHHHHHHHCCCCEECCCCEEEEECCCHHHHHHHH
HTGGDQTGKFLTTFLQEKLTNIHWQEQKMAIEIIKQNDSAIGVHCLDKENRLHTYYGEHI
HCCCCCHHHHHHHHHHHHHHCCCHHHHHHEEHHEECCCCEEEEEEECCCCCEEEEECCEE
ILASGGLGQLFPVTTNAATISGDGLALAYRAGAKLTDMEFIQFHPTLLFLNGRCHGLISE
EEECCCCCCEEEEECCCEEECCCCEEEEEECCCCCCCCHHEEECCEEEEECCCCHHHHHH
AVRGEGAKLIRADGSAVMTDVHPRADLAPRDIVAATLFAEIQDGNEVFLDITPIPNFEER
HHCCCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
FPGITANLDAYHVPFRETKRIPVHPGAHFLMGGIRTDLQGKTNIPGLYAIGEVANAGVHG
CCCCEECCCEEECCHHHCCCCCCCCCHHHHHCCHHCCCCCCCCCCCEEEHHHHHCCCCCC
ANRLASNSLLETLVFGEKVAEYILTQKTNLIDHPEIPLSNQTQTPHLPDKQLLQEKIWET
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
LGITRKPEKITEFLHWLTDFDYANHTRETAEISHMLITAKLIAESALKRTESLGAHRILK
HCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVIK
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]