Click here to switch to the map view.

The map label for this gene is metE [H]

Identifier: 226224284

GI number: 226224284

Start: 1723487

End: 1725784

Strand: Reverse

Name: metE [H]

Synonym: Lm4b_01694

Alternate gene names: 226224284

Gene position: 1725784-1723487 (Counterclockwise)

Preceding gene: 226224285

Following gene: 226224283

Centisome position: 59.25

GC content: 39.99

Gene sequence:

>2298_bases
ATGGTAAAGGCAATTAGTTCAAACTTGGGGTATCCGAGACTTGGAGAGAAACGTGAATGGAAACGTGCACTAGAAAAGTT
TTGGAATGGCACAATTTCAGAAAAGGAATTATTGGCGGAAACAAAGATTCTAAGATTACATGCGCTAAAAAAACAACAAG
ATAAAGGGATTGATTTGATTCCAGTTGGTGACTTTAGTTTTTATGATCAAGTGCTTGATACGAGTGTTACATTTGGGATT
ATTCCAAAACGTTTTCAGCATGATGGAGGGAAAGTTTCGCTAAATACATATTTTGATATTGCACGTGGTAAAAATGACGC
GGTGGCCTCTGAAATGACTAAATGGTTTAATACGAACTATCACTATATTGTTCCAGAACTCGCTGATGCGGAGCCGAAAG
TGTTGGATAATCGGGCACTATACTATTACGAAGAAGCGAAGAAAGAGCTTGGTATTGAAGGAAAACCAGTGCTTGTTGGG
CCAATTACTTATTTAAAACTTGGAAAAGGTAGCGATGCAAAAAGTTTTGAAGTATTGTTAGATAAATTTATTCCGGCGTA
TGTAGAGATTTTGAAAGAACTTGAGGCAGCTGGTGCCAAGTGGGTGCAAATTGACGAGCCATATCTTGCAACTAGTTTTG
ATAAAAAAGAAATTGCGTTATTTGAAAAAGTATATCAAGCGTTTCAAACGGCAGTTCCAAATTTGAAAATAGAGTTACAA
ACTTATTTTGAAAGTTTGGATTATTATGAAGAGGTTGTTAATTTACCAGTTGCGGCGATTGGGATTGACTTTGTTCATGA
TCATGGTGATTCGATAAAAGCGTTAAAAGCTCACGGATTTCCGGAAGATAAGTATTTAGCGGCCGGTGTGGTGGATGGAC
GGAATGTTTGGCGTAGTGACTTGGATGCTAAACTGGAACTACTTACGGAGATTGCGAATTATGTGGCGGATGGAAAATTG
ATTGTTCAACCTTCTAATTCATTGCTCCATGTACCGGTGACGAAGTTAAGTGAGCCAGATTTGGATGAAGTGATTCTTGG
TGGCTTATCATTTGCTGATCAAAAATTAGAGGAGATTACTATTTTGACGAAGGCTTTGACGAATGGTGCGGAAAGTGTGG
CAGCTGAACTAGAAGAATCTCGTGCAGCAGTGACGGCGCTCAATGAATCTAGTCATCGAAATAATTTAGAAGTTCAAGAA
GCGATTGCTAATTTGAAAAATGTGCGGGTGGACCGGGAGTTGCCATTTGCGGAACGGATTAAGTTGCAACATGCTTGGTT
GAAATTACCGCTTTTCCCAACGACAACAATCGGAAGTTTTCCACAATCACCGGAAGTTCGGAAGACGCGCGCTGACTGGT
TAAAAGGGAACATAACCGATGCAGAATATAATGCTTTTATTGAAAAAGAAACGGCTCGCTGGATTAAAATTCAAGAGGAT
TTGGATATTGATGTATTAGTTCACGGGGAATTTGAACGGACAGATATGGTGGAATATTTCGGGCAAAAATTGGCTGGTTT
CCAAGCAACTAAATTTGGATGGGTACAGTCTTATGGTTCGAGAGCGGTTCGTCCACCACTTATCTACGGGGATGTTGCTT
TTACAGAGGAAATTACCGTGAAAGAAAGTGTCTATGCCCAGTCGTTAACGAAGCGCCCAGTGAAGGGAATGCTAACGGCG
CCGGTGACAATTATTAATTGGAGTTTTGTTCGTGATGATGTGCCGGAGAGTGTAGTTGCAAATCAGGTTGGACTTGCGCT
TCGTAAAGAAGTAGAAGCGTTAGAACGTAATGGCATTAAAGTTATCCAAGTGGATGAGCCAGCTTTACGTGAAGGTTTAC
CATTAAAACAAGCAAGATGGCAAAAATATTTAGACGATGCAGTTTATTCGTTTAAATTAACGACTGCTTCGGTCCAAAAT
GATACGCAAATTCATACGCATATGTGTTATTCGGACTTTGACGATATTATTGATACGATTAGTGCTCTAGATGCAGATGT
TATTTCGATTGAAACGTCAAGAAGTCACGGGGAGATAATTTCGACATTTGAGGAAGTGACGTATGATAAGGAAATTGGCC
TTGGGGTTTATGATATTCATAGTCCACGCGTTCCGACTGTGACTGAAATCCAAGACAATATTCGCCGAGCGTTACGTGCA
ATTGATGCAAAACAATTTTGGATTAACCCAGATTGTGGGTTGAAAACGCGTAAAGAGCCTGAAACGATTGCGGCACTTCA
AGATATGATTAAAGCGACTAAAGAAGTTCGTGCGGAATATCAAGTATTAGAGAAATAA

Upstream 100 bases:

>100_bases
AAAAAGTTTCACTGTATTTGTGTATCGAAACTTCCAAACCTCTCTAGTTCTCTAGGGAGGTTTTTTATTGGCAAAAAATG
GAGAGGATAAGGTGATAAGT

Downstream 100 bases:

>100_bases
AGGAATGGAGGAGAGGTAATTATGGCAAAGCTGAAACAAGAGACGATAGCAGCGCAAATTGGAAATAGAAAATGTGAAAG
AACGGGTGCTGTCAATATGC

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 765; Mature: 765

Protein sequence:

>765_residues
MVKAISSNLGYPRLGEKREWKRALEKFWNGTISEKELLAETKILRLHALKKQQDKGIDLIPVGDFSFYDQVLDTSVTFGI
IPKRFQHDGGKVSLNTYFDIARGKNDAVASEMTKWFNTNYHYIVPELADAEPKVLDNRALYYYEEAKKELGIEGKPVLVG
PITYLKLGKGSDAKSFEVLLDKFIPAYVEILKELEAAGAKWVQIDEPYLATSFDKKEIALFEKVYQAFQTAVPNLKIELQ
TYFESLDYYEEVVNLPVAAIGIDFVHDHGDSIKALKAHGFPEDKYLAAGVVDGRNVWRSDLDAKLELLTEIANYVADGKL
IVQPSNSLLHVPVTKLSEPDLDEVILGGLSFADQKLEEITILTKALTNGAESVAAELEESRAAVTALNESSHRNNLEVQE
AIANLKNVRVDRELPFAERIKLQHAWLKLPLFPTTTIGSFPQSPEVRKTRADWLKGNITDAEYNAFIEKETARWIKIQED
LDIDVLVHGEFERTDMVEYFGQKLAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA
PVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQVDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQN
DTQIHTHMCYSDFDDIIDTISALDADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVTEIQDNIRRALRA
IDAKQFWINPDCGLKTRKEPETIAALQDMIKATKEVRAEYQVLEK

Sequences:

>Translated_765_residues
MVKAISSNLGYPRLGEKREWKRALEKFWNGTISEKELLAETKILRLHALKKQQDKGIDLIPVGDFSFYDQVLDTSVTFGI
IPKRFQHDGGKVSLNTYFDIARGKNDAVASEMTKWFNTNYHYIVPELADAEPKVLDNRALYYYEEAKKELGIEGKPVLVG
PITYLKLGKGSDAKSFEVLLDKFIPAYVEILKELEAAGAKWVQIDEPYLATSFDKKEIALFEKVYQAFQTAVPNLKIELQ
TYFESLDYYEEVVNLPVAAIGIDFVHDHGDSIKALKAHGFPEDKYLAAGVVDGRNVWRSDLDAKLELLTEIANYVADGKL
IVQPSNSLLHVPVTKLSEPDLDEVILGGLSFADQKLEEITILTKALTNGAESVAAELEESRAAVTALNESSHRNNLEVQE
AIANLKNVRVDRELPFAERIKLQHAWLKLPLFPTTTIGSFPQSPEVRKTRADWLKGNITDAEYNAFIEKETARWIKIQED
LDIDVLVHGEFERTDMVEYFGQKLAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA
PVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQVDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQN
DTQIHTHMCYSDFDDIIDTISALDADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVTEIQDNIRRALRA
IDAKQFWINPDCGLKTRKEPETIAALQDMIKATKEVRAEYQVLEK
>Mature_765_residues
MVKAISSNLGYPRLGEKREWKRALEKFWNGTISEKELLAETKILRLHALKKQQDKGIDLIPVGDFSFYDQVLDTSVTFGI
IPKRFQHDGGKVSLNTYFDIARGKNDAVASEMTKWFNTNYHYIVPELADAEPKVLDNRALYYYEEAKKELGIEGKPVLVG
PITYLKLGKGSDAKSFEVLLDKFIPAYVEILKELEAAGAKWVQIDEPYLATSFDKKEIALFEKVYQAFQTAVPNLKIELQ
TYFESLDYYEEVVNLPVAAIGIDFVHDHGDSIKALKAHGFPEDKYLAAGVVDGRNVWRSDLDAKLELLTEIANYVADGKL
IVQPSNSLLHVPVTKLSEPDLDEVILGGLSFADQKLEEITILTKALTNGAESVAAELEESRAAVTALNESSHRNNLEVQE
AIANLKNVRVDRELPFAERIKLQHAWLKLPLFPTTTIGSFPQSPEVRKTRADWLKGNITDAEYNAFIEKETARWIKIQED
LDIDVLVHGEFERTDMVEYFGQKLAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITVKESVYAQSLTKRPVKGMLTA
PVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIKVIQVDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQN
DTQIHTHMCYSDFDDIIDTISALDADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVTEIQDNIRRALRA
IDAKQFWINPDCGLKTRKEPETIAALQDMIKATKEVRAEYQVLEK

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=760, Percent_Identity=45.7894736842105, Blast_Score=643, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=777, Percent_Identity=43.5006435006435, Blast_Score=593, Evalue=1e-170,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 86330; Mature: 86330

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKAISSNLGYPRLGEKREWKRALEKFWNGTISEKELLAETKILRLHALKKQQDKGIDLI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
PVGDFSFYDQVLDTSVTFGIIPKRFQHDGGKVSLNTYFDIARGKNDAVASEMTKWFNTNY
ECCCHHHHHHHHHCCCEECCCCHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCC
HYIVPELADAEPKVLDNRALYYYEEAKKELGIEGKPVLVGPITYLKLGKGSDAKSFEVLL
EEECCCCCCCCCCEECCCEEEEEHHHHHHCCCCCCCEEECCEEEEEECCCCCCHHHHHHH
DKFIPAYVEILKELEAAGAKWVQIDEPYLATSFDKKEIALFEKVYQAFQTAVPNLKIELQ
HHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEHH
TYFESLDYYEEVVNLPVAAIGIDFVHDHGDSIKALKAHGFPEDKYLAAGVVDGRNVWRSD
HHHHHHHHHHHHHHCCCCEEEEEHEECCCCCEEHHHHCCCCCCCEEEEEECCCHHHHHHH
LDAKLELLTEIANYVADGKLIVQPSNSLLHVPVTKLSEPDLDEVILGGLSFADQKLEEIT
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHCCCHHHHHHHHHHH
ILTKALTNGAESVAAELEESRAAVTALNESSHRNNLEVQEAIANLKNVRVDRELPFAERI
HHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
KLQHAWLKLPLFPTTTIGSFPQSPEVRKTRADWLKGNITDAEYNAFIEKETARWIKIQED
HHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECC
LDIDVLVHGEFERTDMVEYFGQKLAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITV
CCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCEEECCCHHH
KESVYAQSLTKRPVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIK
HHHHHHHHHHCCCCCCHHCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VIQVDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQNDTQIHTHMCYSDFDDIIDTI
EEEECCHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
SALDADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVTEIQDNIRRALRA
HHCCCCEEEEECCCCCCHHHHHHHHHCCCHHCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IDAKQFWINPDCGLKTRKEPETIAALQDMIKATKEVRAEYQVLEK
HCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVKAISSNLGYPRLGEKREWKRALEKFWNGTISEKELLAETKILRLHALKKQQDKGIDLI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
PVGDFSFYDQVLDTSVTFGIIPKRFQHDGGKVSLNTYFDIARGKNDAVASEMTKWFNTNY
ECCCHHHHHHHHHCCCEECCCCHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHCCCC
HYIVPELADAEPKVLDNRALYYYEEAKKELGIEGKPVLVGPITYLKLGKGSDAKSFEVLL
EEECCCCCCCCCCEECCCEEEEEHHHHHHCCCCCCCEEECCEEEEEECCCCCCHHHHHHH
DKFIPAYVEILKELEAAGAKWVQIDEPYLATSFDKKEIALFEKVYQAFQTAVPNLKIELQ
HHHHHHHHHHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHHHHHHHHHHHCCCCEEEHH
TYFESLDYYEEVVNLPVAAIGIDFVHDHGDSIKALKAHGFPEDKYLAAGVVDGRNVWRSD
HHHHHHHHHHHHHHCCCCEEEEEHEECCCCCEEHHHHCCCCCCCEEEEEECCCHHHHHHH
LDAKLELLTEIANYVADGKLIVQPSNSLLHVPVTKLSEPDLDEVILGGLSFADQKLEEIT
HHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEHHHCCCCCHHHHHHCCCHHHHHHHHHHH
ILTKALTNGAESVAAELEESRAAVTALNESSHRNNLEVQEAIANLKNVRVDRELPFAERI
HHHHHHHCCHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHH
KLQHAWLKLPLFPTTTIGSFPQSPEVRKTRADWLKGNITDAEYNAFIEKETARWIKIQED
HHHHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECC
LDIDVLVHGEFERTDMVEYFGQKLAGFQATKFGWVQSYGSRAVRPPLIYGDVAFTEEITV
CCEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCEEECCCHHH
KESVYAQSLTKRPVKGMLTAPVTIINWSFVRDDVPESVVANQVGLALRKEVEALERNGIK
HHHHHHHHHHCCCCCCHHCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCE
VIQVDEPALREGLPLKQARWQKYLDDAVYSFKLTTASVQNDTQIHTHMCYSDFDDIIDTI
EEEECCHHHHCCCCCHHHHHHHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
SALDADVISIETSRSHGEIISTFEEVTYDKEIGLGVYDIHSPRVPTVTEIQDNIRRALRA
HHCCCCEEEEECCCCCCHHHHHHHHHCCCHHCCCEEEECCCCCCCCHHHHHHHHHHHHHH
IDAKQFWINPDCGLKTRKEPETIAALQDMIKATKEVRAEYQVLEK
HCCHHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]