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The map label for this gene is iscS [C]

Identifier: 226224194

GI number: 226224194

Start: 1618840

End: 1619979

Strand: Reverse

Name: iscS [C]

Synonym: Lm4b_01604

Alternate gene names: 226224194

Gene position: 1619979-1618840 (Counterclockwise)

Preceding gene: 226224195

Following gene: 226224193

Centisome position: 55.62

GC content: 40.0

Gene sequence:

>1140_bases
ATGATTTATTTCGATAATAGTGCGACAACAAAGCCAAATGCGGCCGTACTTGAAACATATACAAAGGTAGCGAGTAATTA
TTTTGCTAATCCTTCTTCGCTGCATCGTTTTGGTGCTAAGTCGAAAGAGCTGCTTGATGCATCAAGAAAACAAATCGCTG
CGATGGTGAGCGCTTTGCCAGAAGAAATTATTTTTACATCTGGTGGCACAGAGGGGAATAATTTGGCGATTAAAGGGCTC
GTTTATAGTTACCAAAATCGCGGGAAACATATTGTTACCTCTAGCATCGAGCATCCTTCTGTTCGTATGGTAATGGAAGA
ACTTGAGCAGGAAGGTTTTACTGTCACGTATTTACCAGTAGATAAAAACGGCGTCATCAAGATGGAAGCATTGAAAGCTG
CACTCACAGATGAAACGATTTTAGTGTCTATTATGGGCGTGAACAATGAGGTGGGAAGCATCCAACCACTGCACGAAATT
GGCGAAATGCTAAAATTGCTTGAACATACTTTCTTTCATGTAGATTTCGTTCAAGGTATCGGAAAAATACCACTTGCACT
TAATGAGAATGCTATTGATTTGCTCACTTTTTCAGGGCATAAATTTCATGCTCTACGTGGAACCGGAATACTTTTTAAAC
GAAAAAATGTGCATTTGCATTCAGAAATTCTTGGTGGTGGGCAGGAAATGGGCTACCGCAGTGGAACAGAAAATTTAGCT
GGGAATGTTGCGCTGGCAAAAGCATTACGACTAACGCTCGAAAATGAGCCACGAAAAGAAGCATTAATCGAGATTCGCGA
TTATTTACTAACGGAAATTGCGCAAATGTCTGATATGACAGTTCATACGAAACAAAGTGTCGCGGCTCCGCATATCGTTT
GTTTTTCGGCAAAAGGGCATCGTGGGGAAATTTTAGTACATGCTTTAGAAAAAGAAGATATTTACATTTCCACCACTAGT
GCGTGTTCTTCTAAGCAGAAGCTAGCAAGCAGCACGTTAAAAGCGATGGGTGTGACGGACGAAGAAGCAACAGGAGCAGT
ACGTGTTAGCTTATCGTATGAAAATCGTTTATCTGAAGCGAAAATTTTTATTCAAAAACTGCAAGAAATCATTGAAAATC
TAAATAAAGTGGTGAAATAA

Upstream 100 bases:

>100_bases
TATAATAGGAATCCTGTTTTTTTATGAAAATAGTAGTTGGAAAGTGTTGGAATTATGATAAAATGGAGAGTGGAATATTG
GGGAGGAGAATTTAGTTTTT

Downstream 100 bases:

>100_bases
ATTGGAATTTGATCGTATGTTAATTAGATACGGAGAGTTATCTACAAAAGGAAAAAACAGAAAACAATTTGTGACAAAGT
TGGCGCAAAACGTCAAACGT

Product: iron-sulfur cofactor synthesis protein nifS

Products: L-alanine; S [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIYFDNSATTKPNAAVLETYTKVASNYFANPSSLHRFGAKSKELLDASRKQIAAMVSALPEEIIFTSGGTEGNNLAIKGL
VYSYQNRGKHIVTSSIEHPSVRMVMEELEQEGFTVTYLPVDKNGVIKMEALKAALTDETILVSIMGVNNEVGSIQPLHEI
GEMLKLLEHTFFHVDFVQGIGKIPLALNENAIDLLTFSGHKFHALRGTGILFKRKNVHLHSEILGGGQEMGYRSGTENLA
GNVALAKALRLTLENEPRKEALIEIRDYLLTEIAQMSDMTVHTKQSVAAPHIVCFSAKGHRGEILVHALEKEDIYISTTS
ACSSKQKLASSTLKAMGVTDEEATGAVRVSLSYENRLSEAKIFIQKLQEIIENLNKVVK

Sequences:

>Translated_379_residues
MIYFDNSATTKPNAAVLETYTKVASNYFANPSSLHRFGAKSKELLDASRKQIAAMVSALPEEIIFTSGGTEGNNLAIKGL
VYSYQNRGKHIVTSSIEHPSVRMVMEELEQEGFTVTYLPVDKNGVIKMEALKAALTDETILVSIMGVNNEVGSIQPLHEI
GEMLKLLEHTFFHVDFVQGIGKIPLALNENAIDLLTFSGHKFHALRGTGILFKRKNVHLHSEILGGGQEMGYRSGTENLA
GNVALAKALRLTLENEPRKEALIEIRDYLLTEIAQMSDMTVHTKQSVAAPHIVCFSAKGHRGEILVHALEKEDIYISTTS
ACSSKQKLASSTLKAMGVTDEEATGAVRVSLSYENRLSEAKIFIQKLQEIIENLNKVVK
>Mature_379_residues
MIYFDNSATTKPNAAVLETYTKVASNYFANPSSLHRFGAKSKELLDASRKQIAAMVSALPEEIIFTSGGTEGNNLAIKGL
VYSYQNRGKHIVTSSIEHPSVRMVMEELEQEGFTVTYLPVDKNGVIKMEALKAALTDETILVSIMGVNNEVGSIQPLHEI
GEMLKLLEHTFFHVDFVQGIGKIPLALNENAIDLLTFSGHKFHALRGTGILFKRKNVHLHSEILGGGQEMGYRSGTENLA
GNVALAKALRLTLENEPRKEALIEIRDYLLTEIAQMSDMTVHTKQSVAAPHIVCFSAKGHRGEILVHALEKEDIYISTTS
ACSSKQKLASSTLKAMGVTDEEATGAVRVSLSYENRLSEAKIFIQKLQEIIENLNKVVK

Specific function: Fe-S cluster biosynthesis [C]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=35.9580052493438, Blast_Score=232, Evalue=4e-61,
Organism=Homo sapiens, GI156713448, Length=411, Percent_Identity=31.6301703163017, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI48994898, Length=356, Percent_Identity=41.2921348314607, Blast_Score=255, Evalue=3e-69,
Organism=Escherichia coli, GI1787970, Length=396, Percent_Identity=23.7373737373737, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789175, Length=279, Percent_Identity=26.1648745519713, Blast_Score=69, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25143064, Length=396, Percent_Identity=36.6161616161616, Blast_Score=224, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=33.0246913580247, Blast_Score=149, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=380, Percent_Identity=34.7368421052632, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI20129463, Length=393, Percent_Identity=35.6234096692112, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: NA

Molecular weight: Translated: 41669; Mature: 41669

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNSATTKPNAAVLETYTKVASNYFANPSSLHRFGAKSKELLDASRKQIAAMVSALP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
EEIIFTSGGTEGNNLAIKGLVYSYQNRGKHIVTSSIEHPSVRMVMEELEQEGFTVTYLPV
HHEEEECCCCCCCCEEEEEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEE
DKNGVIKMEALKAALTDETILVSIMGVNNEVGSIQPLHEIGEMLKLLEHTFFHVDFVQGI
CCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GKIPLALNENAIDLLTFSGHKFHALRGTGILFKRKNVHLHSEILGGGQEMGYRSGTENLA
CCCCEEECCCCEEEEEECCCEEEEEECCEEEEEECCCEEHHHHHCCCHHCCCCCCCCHHH
GNVALAKALRLTLENEPRKEALIEIRDYLLTEIAQMSDMTVHTKQSVAAPHIVCFSAKGH
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCEEEEEECCCC
RGEILVHALEKEDIYISTTSACSSKQKLASSTLKAMGVTDEEATGAVRVSLSYENRLSEA
CCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHH
KIFIQKLQEIIENLNKVVK
HHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIYFDNSATTKPNAAVLETYTKVASNYFANPSSLHRFGAKSKELLDASRKQIAAMVSALP
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
EEIIFTSGGTEGNNLAIKGLVYSYQNRGKHIVTSSIEHPSVRMVMEELEQEGFTVTYLPV
HHEEEECCCCCCCCEEEEEEEEEHHCCCCEEEECCCCCCHHHHHHHHHHHCCCEEEEEEE
DKNGVIKMEALKAALTDETILVSIMGVNNEVGSIQPLHEIGEMLKLLEHTFFHVDFVQGI
CCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GKIPLALNENAIDLLTFSGHKFHALRGTGILFKRKNVHLHSEILGGGQEMGYRSGTENLA
CCCCEEECCCCEEEEEECCCEEEEEECCEEEEEECCCEEHHHHHCCCHHCCCCCCCCHHH
GNVALAKALRLTLENEPRKEALIEIRDYLLTEIAQMSDMTVHTKQSVAAPHIVCFSAKGH
CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEECHHHCCCCEEEEEECCCC
RGEILVHALEKEDIYISTTSACSSKQKLASSTLKAMGVTDEEATGAVRVSLSYENRLSEA
CCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHH
KIFIQKLQEIIENLNKVVK
HHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal Phosphate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-cysteine [C]

Specific reaction: L-cysteine = L-alanine + S [C]

General reaction: transfers sulfane sulfur to ThiI during in vitro biosynthesis of 4-thiouridine in E. coli tRNA [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8031904 [H]