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The map label for this gene is thiI [H]

Identifier: 226224193

GI number: 226224193

Start: 1617627

End: 1618838

Strand: Reverse

Name: thiI [H]

Synonym: Lm4b_01603

Alternate gene names: 226224193

Gene position: 1618838-1617627 (Counterclockwise)

Preceding gene: 226224194

Following gene: 226224192

Centisome position: 55.58

GC content: 39.85

Gene sequence:

>1212_bases
TTGGAATTTGATCGTATGTTAATTAGATACGGAGAGTTATCTACAAAAGGAAAAAACAGAAAACAATTTGTGACAAAGTT
GGCGCAAAACGTCAAACGTGCAATGACAGATTTGCCGGAAGTTCGTATTCACGGAGAACGTGACCGAATGTATATTATTT
TAAATGGGGCAGATTATCAGCTCGTAGAAGAACGTTTAAAACCGATTTTCGGAATCCAATCCTTTAGTCCAGCTGTACGC
GTCAATTTAGATGTAGAGGAAGTAAAAGCCGCTGCACTTGCCTTAGTACAAGATGCGCATGAGGAGAACGGAACATTTAA
AGTCGCAGCAAGACGTAGCCACCGCGAGTTTCCGCTTGATTCTAATGAAATCAACCAAGAAATCGGTGCTTATGTATTAC
AAAATATGGAAGATTTAACGGTCAATGTTAAAAATCCTGATGTAAAACTAACCATTGATGTGCGTAAAGAAGGCGTATTT
TTATCATGCCGGACAATTCTTGGTGCAGCTGGTCTGCCAGTAGGTTCATCGGGTCGTGCAATGTTAATGCTTTCGGGAGG
TATTGATAGTCCGGTTGCAGGCTATTTAGCCCAAAAACGTGGCGTAGAAATTGAAGCAGTTCATTTTCATAGCCCACCGT
ATACAAGTGAACAAGCGAAACAAAAGGCGGTTGATTTAGCTGCAAAATTGGCTAAATACAGCGGACAGGTACAAATGCAC
ATCGTTCCATTTACAGAGATTCAAGAAGTGATCAAACAACAAATTCCTGAAAGTGTGATTATGACCGTAACTCGTCGAAT
GATGCTTCGAATTACGGATGAACTTCGTCGCAGGAGAAACGGTTTAGCGATTGTTAATGGCGAAAGTTTAGGGCAAGTTG
CCAGCCAAACATTGGAAAGTATGCTAGCAATTAACGCCGTAACAGCGACACCAATCATTCGTCCAGTCGTATCCATGGAT
AAAAATGAAATCATTCAAATCGCGCAAAAGATTGATACGTATAATTTATCAGTGCAACCATTTGAAGATTGCTGTACCAT
TTTCACGCCACCATCTCCAAAAACAAAACCAAAACTAGATAAAATCGAGCATTACGAAAGTTTCACAGATTTTGAAGCGT
TAATTGCGAAAGCATTAGACAACATTGAAACCATTTCTGTGAATGTAGCGGAAACTGCACAAGTCAAAGATGAGTTTGCT
GATTTATTTTAA

Upstream 100 bases:

>100_bases
CGTGTTAGCTTATCGTATGAAAATCGTTTATCTGAAGCGAAAATTTTTATTCAAAAACTGCAAGAAATCATTGAAAATCT
AAATAAAGTGGTGAAATAAA

Downstream 100 bases:

>100_bases
GATTAACCGTTTATCCTTTTCTGGATAAACGGTTTTTGTATATTATATACATAATAAATAACTAAAAATGCATAAAATAG
TTTTAAATATTAATAAAAAA

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MEFDRMLIRYGELSTKGKNRKQFVTKLAQNVKRAMTDLPEVRIHGERDRMYIILNGADYQLVEERLKPIFGIQSFSPAVR
VNLDVEEVKAAALALVQDAHEENGTFKVAARRSHREFPLDSNEINQEIGAYVLQNMEDLTVNVKNPDVKLTIDVRKEGVF
LSCRTILGAAGLPVGSSGRAMLMLSGGIDSPVAGYLAQKRGVEIEAVHFHSPPYTSEQAKQKAVDLAAKLAKYSGQVQMH
IVPFTEIQEVIKQQIPESVIMTVTRRMMLRITDELRRRRNGLAIVNGESLGQVASQTLESMLAINAVTATPIIRPVVSMD
KNEIIQIAQKIDTYNLSVQPFEDCCTIFTPPSPKTKPKLDKIEHYESFTDFEALIAKALDNIETISVNVAETAQVKDEFA
DLF

Sequences:

>Translated_403_residues
MEFDRMLIRYGELSTKGKNRKQFVTKLAQNVKRAMTDLPEVRIHGERDRMYIILNGADYQLVEERLKPIFGIQSFSPAVR
VNLDVEEVKAAALALVQDAHEENGTFKVAARRSHREFPLDSNEINQEIGAYVLQNMEDLTVNVKNPDVKLTIDVRKEGVF
LSCRTILGAAGLPVGSSGRAMLMLSGGIDSPVAGYLAQKRGVEIEAVHFHSPPYTSEQAKQKAVDLAAKLAKYSGQVQMH
IVPFTEIQEVIKQQIPESVIMTVTRRMMLRITDELRRRRNGLAIVNGESLGQVASQTLESMLAINAVTATPIIRPVVSMD
KNEIIQIAQKIDTYNLSVQPFEDCCTIFTPPSPKTKPKLDKIEHYESFTDFEALIAKALDNIETISVNVAETAQVKDEFA
DLF
>Mature_403_residues
MEFDRMLIRYGELSTKGKNRKQFVTKLAQNVKRAMTDLPEVRIHGERDRMYIILNGADYQLVEERLKPIFGIQSFSPAVR
VNLDVEEVKAAALALVQDAHEENGTFKVAARRSHREFPLDSNEINQEIGAYVLQNMEDLTVNVKNPDVKLTIDVRKEGVF
LSCRTILGAAGLPVGSSGRAMLMLSGGIDSPVAGYLAQKRGVEIEAVHFHSPPYTSEQAKQKAVDLAAKLAKYSGQVQMH
IVPFTEIQEVIKQQIPESVIMTVTRRMMLRITDELRRRRNGLAIVNGESLGQVASQTLESMLAINAVTATPIIRPVVSMD
KNEIIQIAQKIDTYNLSVQPFEDCCTIFTPPSPKTKPKLDKIEHYESFTDFEALIAKALDNIETISVNVAETAQVKDEFA
DLF

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=306, Percent_Identity=29.7385620915033, Blast_Score=123, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 45030; Mature: 45030

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDRMLIRYGELSTKGKNRKQFVTKLAQNVKRAMTDLPEVRIHGERDRMYIILNGADYQ
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCHH
LVEERLKPIFGIQSFSPAVRVNLDVEEVKAAALALVQDAHEENGTFKVAARRSHREFPLD
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
SNEINQEIGAYVLQNMEDLTVNVKNPDVKLTIDVRKEGVFLSCRTILGAAGLPVGSSGRA
HHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCEEEEHHHHHCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLAQKRGVEIEAVHFHSPPYTSEQAKQKAVDLAAKLAKYSGQVQMH
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTEIQEVIKQQIPESVIMTVTRRMMLRITDELRRRRNGLAIVNGESLGQVASQTLES
EECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MLAINAVTATPIIRPVVSMDKNEIIQIAQKIDTYNLSVQPFEDCCTIFTPPSPKTKPKLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCCCCHH
KIEHYESFTDFEALIAKALDNIETISVNVAETAQVKDEFADLF
HHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEFDRMLIRYGELSTKGKNRKQFVTKLAQNVKRAMTDLPEVRIHGERDRMYIILNGADYQ
CCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCHH
LVEERLKPIFGIQSFSPAVRVNLDVEEVKAAALALVQDAHEENGTFKVAARRSHREFPLD
HHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
SNEINQEIGAYVLQNMEDLTVNVKNPDVKLTIDVRKEGVFLSCRTILGAAGLPVGSSGRA
HHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEECCCEEEEHHHHHCCCCCCCCCCCCE
MLMLSGGIDSPVAGYLAQKRGVEIEAVHFHSPPYTSEQAKQKAVDLAAKLAKYSGQVQMH
EEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTEIQEVIKQQIPESVIMTVTRRMMLRITDELRRRRNGLAIVNGESLGQVASQTLES
EECHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHH
MLAINAVTATPIIRPVVSMDKNEIIQIAQKIDTYNLSVQPFEDCCTIFTPPSPKTKPKLD
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCCCCCCHH
KIEHYESFTDFEALIAKALDNIETISVNVAETAQVKDEFADLF
HHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]