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The map label for this gene is iscS1 [H]

Identifier: 226224114

GI number: 226224114

Start: 1528771

End: 1529919

Strand: Reverse

Name: iscS1 [H]

Synonym: Lm4b_01523

Alternate gene names: 226224114

Gene position: 1529919-1528771 (Counterclockwise)

Preceding gene: 226224115

Following gene: 226224113

Centisome position: 52.53

GC content: 39.25

Gene sequence:

>1149_bases
ATGGAAAATAGAATTTACTTAGACCATGCTGCGACAAGTCCAATTCATCCAGAAGTTATCCAAACAATGCTTGGCGCAAT
TACAAATACGTACGGCAATCCTTCCAGTATCCATTACGCTGGACGAGAGGCAAGAAAAGCTTTGGATGAAGCTCGTCATA
CAATTGCAGATAGTATCCATGCTATAGAAAAAGAAATAATTTTTACAAGTGGTGGAACAGAAGGCGATAACTTGGCGATT
ATCGGAGCAGCTTTAGCACAGCAAGAAAGTGGTAAACATATTATTACTTCTCAAATTGAGCATCACGCTGTCTTAAAAAC
ATGTGAATACCTTGAAACGCAAGGATTTGAAGTTACCTATTTGCCAGTAGATGAGCATGGAATAGTATCTATAGAGAGTT
TACAAGAGGCCTTGCGTCCAGATACAATTCTAGTTTCTATTATGTATGGAAATAATGAGATAGGCACGATTCAACCGATT
GCGGAGATTGGTGCAGTGTTGCGCAATCATCAAGCAATTTTTCATACAGATGCAGTACAAGCTTATGGGCTATTAAATAT
AAATGTAACGGAGCTAGGGGTTGATTTATTAACTACTTCTTCTCATAAAATAAATGGACCACGTGGCGTAGGTTTTTTAT
ACGTCAAAAATGGCACACGGCTTATCTACCAAATGCACGGCGGAGAACAAGAACGAAAACGTCGCGCTGGAACGGAGAAT
CTAGCGGGAATCTGTGGTTTTAGTGCTGCTTCTTCTATCATGATGAATGAACGAGAGCTAAAAAATGAGGAATATATTTC
CTTCAAAAAACGAATGGCAGAAATTTGGCGTTCGGCAGATCTTGATTTTGAAGTGAATGGATTAGAAGCCAATACCTTGC
CACATGTATTTAGTGTGCGTTTTCCAGGAGTTTCGATTGAACAATTATTAATGAATTTAGATATGGACGGAATTGCAGTT
TCTAGTGGTTCGGCTTGTACGGCGGGAACAGTAGATCCATCGCATGTATTAGTAGCTCTTTTTGGTGAAAATCACCCAGC
GATTCAAGAGACCGTTCGGATTAGTTTTGGACTAGGAAACCATTTAGAAGAAGTGGAAATGGCGGCTACGAAAATTAGTG
AAGTAGTAACGCGTTTAATGAAAATATAA

Upstream 100 bases:

>100_bases
TAGGAGTTATTCCTCTTTACTTTACAGGAGTATTGCTGTTACAATAAGAAACGATTAACGGCGAACAGGAATTCGTCTAC
AAAAAAGGACGCGATAAAAA

Downstream 100 bases:

>100_bases
GAAGGTGAGGAGCGAACTTTGAGTACAAATAATAGTGACATTCGTGTAGTTGTCGGCATGTCAGGCGGAGTAGATTCATC
AGTCACTGCTCATATATTAA

Product: iron-sulfur cofactor synthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MENRIYLDHAATSPIHPEVIQTMLGAITNTYGNPSSIHYAGREARKALDEARHTIADSIHAIEKEIIFTSGGTEGDNLAI
IGAALAQQESGKHIITSQIEHHAVLKTCEYLETQGFEVTYLPVDEHGIVSIESLQEALRPDTILVSIMYGNNEIGTIQPI
AEIGAVLRNHQAIFHTDAVQAYGLLNINVTELGVDLLTTSSHKINGPRGVGFLYVKNGTRLIYQMHGGEQERKRRAGTEN
LAGICGFSAASSIMMNERELKNEEYISFKKRMAEIWRSADLDFEVNGLEANTLPHVFSVRFPGVSIEQLLMNLDMDGIAV
SSGSACTAGTVDPSHVLVALFGENHPAIQETVRISFGLGNHLEEVEMAATKISEVVTRLMKI

Sequences:

>Translated_382_residues
MENRIYLDHAATSPIHPEVIQTMLGAITNTYGNPSSIHYAGREARKALDEARHTIADSIHAIEKEIIFTSGGTEGDNLAI
IGAALAQQESGKHIITSQIEHHAVLKTCEYLETQGFEVTYLPVDEHGIVSIESLQEALRPDTILVSIMYGNNEIGTIQPI
AEIGAVLRNHQAIFHTDAVQAYGLLNINVTELGVDLLTTSSHKINGPRGVGFLYVKNGTRLIYQMHGGEQERKRRAGTEN
LAGICGFSAASSIMMNERELKNEEYISFKKRMAEIWRSADLDFEVNGLEANTLPHVFSVRFPGVSIEQLLMNLDMDGIAV
SSGSACTAGTVDPSHVLVALFGENHPAIQETVRISFGLGNHLEEVEMAATKISEVVTRLMKI
>Mature_382_residues
MENRIYLDHAATSPIHPEVIQTMLGAITNTYGNPSSIHYAGREARKALDEARHTIADSIHAIEKEIIFTSGGTEGDNLAI
IGAALAQQESGKHIITSQIEHHAVLKTCEYLETQGFEVTYLPVDEHGIVSIESLQEALRPDTILVSIMYGNNEIGTIQPI
AEIGAVLRNHQAIFHTDAVQAYGLLNINVTELGVDLLTTSSHKINGPRGVGFLYVKNGTRLIYQMHGGEQERKRRAGTEN
LAGICGFSAASSIMMNERELKNEEYISFKKRMAEIWRSADLDFEVNGLEANTLPHVFSVRFPGVSIEQLLMNLDMDGIAV
SSGSACTAGTVDPSHVLVALFGENHPAIQETVRISFGLGNHLEEVEMAATKISEVVTRLMKI

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=381, Percent_Identity=39.6325459317585, Blast_Score=272, Evalue=3e-73,
Organism=Homo sapiens, GI156713448, Length=419, Percent_Identity=32.4582338902148, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=44.1860465116279, Blast_Score=283, Evalue=2e-77,
Organism=Escherichia coli, GI1787970, Length=267, Percent_Identity=29.5880149812734, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1789175, Length=384, Percent_Identity=25.78125, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=371, Percent_Identity=42.8571428571429, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=29.0123456790123, Blast_Score=134, Evalue=6e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=384, Percent_Identity=41.9270833333333, Blast_Score=279, Evalue=4e-76,
Organism=Drosophila melanogaster, GI20129463, Length=382, Percent_Identity=40.0523560209424, Blast_Score=261, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 41784; Mature: 41784

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRIYLDHAATSPIHPEVIQTMLGAITNTYGNPSSIHYAGREARKALDEARHTIADSIH
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
AIEKEIIFTSGGTEGDNLAIIGAALAQQESGKHIITSQIEHHAVLKTCEYLETQGFEVTY
HHHHEEEEECCCCCCCCEEEEEHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEE
LPVDEHGIVSIESLQEALRPDTILVSIMYGNNEIGTIQPIAEIGAVLRNHQAIFHTDAVQ
EECCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
AYGLLNINVTELGVDLLTTSSHKINGPRGVGFLYVKNGTRLIYQMHGGEQERKRRAGTEN
HEEEEEEEHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCH
LAGICGFSAASSIMMNERELKNEEYISFKKRMAEIWRSADLDFEVNGLEANTLPHVFSVR
HHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
FPGVSIEQLLMNLDMDGIAVSSGSACTAGTVDPSHVLVALFGENHPAIQETVRISFGLGN
CCCCCHHHHHHHCCCCCEEECCCCCCEECCCCCCCEEEEEECCCCCCHHHHEEEEECCCC
HLEEVEMAATKISEVVTRLMKI
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MENRIYLDHAATSPIHPEVIQTMLGAITNTYGNPSSIHYAGREARKALDEARHTIADSIH
CCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHH
AIEKEIIFTSGGTEGDNLAIIGAALAQQESGKHIITSQIEHHAVLKTCEYLETQGFEVTY
HHHHEEEEECCCCCCCCEEEEEHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEE
LPVDEHGIVSIESLQEALRPDTILVSIMYGNNEIGTIQPIAEIGAVLRNHQAIFHTDAVQ
EECCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHH
AYGLLNINVTELGVDLLTTSSHKINGPRGVGFLYVKNGTRLIYQMHGGEQERKRRAGTEN
HEEEEEEEHHHHCEEEEECCCCCCCCCCCEEEEEEECCCEEEEEECCCHHHHHHHCCCCH
LAGICGFSAASSIMMNERELKNEEYISFKKRMAEIWRSADLDFEVNGLEANTLPHVFSVR
HHHHHCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEEE
FPGVSIEQLLMNLDMDGIAVSSGSACTAGTVDPSHVLVALFGENHPAIQETVRISFGLGN
CCCCCHHHHHHHCCCCCEEECCCCCCEECCCCCCCEEEEEECCCCCCHHHHEEEEECCCC
HLEEVEMAATKISEVVTRLMKI
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]