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The map label for this gene is yclK [H]

Identifier: 226224109

GI number: 226224109

Start: 1522194

End: 1523633

Strand: Direct

Name: yclK [H]

Synonym: Lm4b_01518

Alternate gene names: 226224109

Gene position: 1522194-1523633 (Clockwise)

Preceding gene: 226224108

Following gene: 226224116

Centisome position: 52.26

GC content: 34.51

Gene sequence:

>1440_bases
ATGAAGCGAATGAAATTTAAATATGCTTACCAATTATTTTTCACACAATTTATCATTTTGCTTATTGCTTGTATCATGAT
TGGGGTGCTTGTCTCTCACTCCTTAAAAGATTATTTTTACCAAAGCCAAGTAGATGACTTAACAAGTTATGGGCAAACGA
TTTCTAGGGATATTCGCTATTCGCCACAAGATGCAACGATGCAAGTTTTGAATACGTATCAGCGAATTTTAGATGTGAAA
AAAATTCATTATACAATCAAAAACGCCAACGATGAAACCATTTATCCAACACAGATGAATCAGCCGTTACCAAAGGATTT
CTCTATTTCTTCGGATGATAAGAAAAAACTTGAAAGTGGCGAAACAGTTAGTAAGAAAATAGATAATCGCTTTAATAAAG
AAATGACAATTGTGTACGTCCCAATAATGAATGGCGACAAATTTGTCGGTTCTATCGTGCTCAATTCACCTATTAGCGGT
ACGGAGCAAGTAATTGGTACGATTAACCGCTATATGTTCTACACTATTTTACTTTCTATAACGGTAGCACTTATTCTTAG
CGCAATCTTGTCCAAACTACAAGTAAATCGAATCAACAAACTACGAGCAGCGACAAAAGACGTTATTCAAGGCAATTACA
AAGCTCGATTGAAGGAAAATAATTTTGATGAAATTGGTGCACTTGCCATTGATTTCAATAAAATGACACAAACCCTTGAA
ACATCTCAAGAAGAAATAGAACGACAAGAGAAGCGGAGACGCCAGTTTATTGCTGATGTTTCTCATGAAATGCGTACCCC
GCTCACAACGATTAGTGGCCTCACGGAAGGTTTAGTAAATGATATTATTCCAAAAAGTGAAACAGATCGTTGCATAGCAC
TCATTGATACAGAAGCTAGACGTTTAACGAAACTAGTCAATGAAAATTTAGATTATGAAAAAATCCGCAGTAACAAAATT
AAACTTCAAAAAACACGTTTTAATGGTAAGGAATTCCTTGAGTTAATCAAAGAACAACTTGATTATGTATCAAGCGAAAA
AGGTAACACTATTACTGTTTCTATCGATAAAGACATGGCGATTTACGCAGATTACGATCGTTTAACACAAGTATTTATCA
ATATCGTCAAAAATAGTGTCCAATTTACTGAAAATGGTCAGATTACTCTAACTGGCACACAAGATTATAAAGAATCTGTT
TTAACGATAACAGATACTGGGATTGGTATGAATACAGAGGAATTAGAACAAATTTGGGAAAGGTTTTATAAAGCAGATAT
GTCTCGAACAAATACCGCTTTTGGCGAATCTGGTATTGGCTTATCCATTGTGAAGCAGTTAATTGAATATCACGATGGAT
CAATTACAGTGACTAGTGAACCAAATAAAGGTACTTCATTTACTATTCGCCTTCCTTTTTTCCAAGATAACGAACAATAA

Upstream 100 bases:

>100_bases
TGGACGTTCACATCAAACGTTTACGCCAAAAAATCGCCACCGAAACAAAGCCATTTTTGCACACCGTCTGGGGTGTTGGC
TACAAATTTGATGAAACGGA

Downstream 100 bases:

>100_bases
AAAAAGAACCACTTTCTATTTTTTTAGAGAGTGGTTCTTTTTTATCCTGCCATAAATTGTTCTGGAGTAATCCCTTCCAT
TCCAATCATCGGATCAATTT

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MKRMKFKYAYQLFFTQFIILLIACIMIGVLVSHSLKDYFYQSQVDDLTSYGQTISRDIRYSPQDATMQVLNTYQRILDVK
KIHYTIKNANDETIYPTQMNQPLPKDFSISSDDKKKLESGETVSKKIDNRFNKEMTIVYVPIMNGDKFVGSIVLNSPISG
TEQVIGTINRYMFYTILLSITVALILSAILSKLQVNRINKLRAATKDVIQGNYKARLKENNFDEIGALAIDFNKMTQTLE
TSQEEIERQEKRRRQFIADVSHEMRTPLTTISGLTEGLVNDIIPKSETDRCIALIDTEARRLTKLVNENLDYEKIRSNKI
KLQKTRFNGKEFLELIKEQLDYVSSEKGNTITVSIDKDMAIYADYDRLTQVFINIVKNSVQFTENGQITLTGTQDYKESV
LTITDTGIGMNTEELEQIWERFYKADMSRTNTAFGESGIGLSIVKQLIEYHDGSITVTSEPNKGTSFTIRLPFFQDNEQ

Sequences:

>Translated_479_residues
MKRMKFKYAYQLFFTQFIILLIACIMIGVLVSHSLKDYFYQSQVDDLTSYGQTISRDIRYSPQDATMQVLNTYQRILDVK
KIHYTIKNANDETIYPTQMNQPLPKDFSISSDDKKKLESGETVSKKIDNRFNKEMTIVYVPIMNGDKFVGSIVLNSPISG
TEQVIGTINRYMFYTILLSITVALILSAILSKLQVNRINKLRAATKDVIQGNYKARLKENNFDEIGALAIDFNKMTQTLE
TSQEEIERQEKRRRQFIADVSHEMRTPLTTISGLTEGLVNDIIPKSETDRCIALIDTEARRLTKLVNENLDYEKIRSNKI
KLQKTRFNGKEFLELIKEQLDYVSSEKGNTITVSIDKDMAIYADYDRLTQVFINIVKNSVQFTENGQITLTGTQDYKESV
LTITDTGIGMNTEELEQIWERFYKADMSRTNTAFGESGIGLSIVKQLIEYHDGSITVTSEPNKGTSFTIRLPFFQDNEQ
>Mature_479_residues
MKRMKFKYAYQLFFTQFIILLIACIMIGVLVSHSLKDYFYQSQVDDLTSYGQTISRDIRYSPQDATMQVLNTYQRILDVK
KIHYTIKNANDETIYPTQMNQPLPKDFSISSDDKKKLESGETVSKKIDNRFNKEMTIVYVPIMNGDKFVGSIVLNSPISG
TEQVIGTINRYMFYTILLSITVALILSAILSKLQVNRINKLRAATKDVIQGNYKARLKENNFDEIGALAIDFNKMTQTLE
TSQEEIERQEKRRRQFIADVSHEMRTPLTTISGLTEGLVNDIIPKSETDRCIALIDTEARRLTKLVNENLDYEKIRSNKI
KLQKTRFNGKEFLELIKEQLDYVSSEKGNTITVSIDKDMAIYADYDRLTQVFINIVKNSVQFTENGQITLTGTQDYKESV
LTITDTGIGMNTEELEQIWERFYKADMSRTNTAFGESGIGLSIVKQLIEYHDGSITVTSEPNKGTSFTIRLPFFQDNEQ

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=280, Percent_Identity=31.0714285714286, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=30.6122448979592, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=28.6792452830189, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786600, Length=229, Percent_Identity=28.3842794759825, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=31.0924369747899, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1788713, Length=245, Percent_Identity=28.5714285714286, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI48994928, Length=230, Percent_Identity=29.1304347826087, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081816, Length=276, Percent_Identity=27.536231884058, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790346, Length=278, Percent_Identity=26.6187050359712, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1786783, Length=463, Percent_Identity=23.7580993520518, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1790861, Length=392, Percent_Identity=23.7244897959184, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=25.8064516129032, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1786912, Length=244, Percent_Identity=26.6393442622951, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082128, Length=234, Percent_Identity=26.0683760683761, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1788549, Length=281, Percent_Identity=27.7580071174377, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1790300, Length=265, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1787894, Length=283, Percent_Identity=21.9081272084806, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 54983; Mature: 54983

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRMKFKYAYQLFFTQFIILLIACIMIGVLVSHSLKDYFYQSQVDDLTSYGQTISRDIRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPQDATMQVLNTYQRILDVKKIHYTIKNANDETIYPTQMNQPLPKDFSISSDDKKKLESG
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCC
ETVSKKIDNRFNKEMTIVYVPIMNGDKFVGSIVLNSPISGTEQVIGTINRYMFYTILLSI
CHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
TVALILSAILSKLQVNRINKLRAATKDVIQGNYKARLKENNFDEIGALAIDFNKMTQTLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHEEEEEHHHHHHHHH
TSQEEIERQEKRRRQFIADVSHEMRTPLTTISGLTEGLVNDIIPKSETDRCIALIDTEAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHH
RLTKLVNENLDYEKIRSNKIKLQKTRFNGKEFLELIKEQLDYVSSEKGNTITVSIDKDMA
HHHHHHHCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
IYADYDRLTQVFINIVKNSVQFTENGQITLTGTQDYKESVLTITDTGIGMNTEELEQIWE
EEECHHHHHHHHHHHHHHCCEEECCCEEEEEECHHHHCCEEEEEECCCCCCHHHHHHHHH
RFYKADMSRTNTAFGESGIGLSIVKQLIEYHDGSITVTSEPNKGTSFTIRLPFFQDNEQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MKRMKFKYAYQLFFTQFIILLIACIMIGVLVSHSLKDYFYQSQVDDLTSYGQTISRDIRY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SPQDATMQVLNTYQRILDVKKIHYTIKNANDETIYPTQMNQPLPKDFSISSDDKKKLESG
CCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCEECCCCCCCCCCCCCCCCCCHHHHHHCC
ETVSKKIDNRFNKEMTIVYVPIMNGDKFVGSIVLNSPISGTEQVIGTINRYMFYTILLSI
CHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
TVALILSAILSKLQVNRINKLRAATKDVIQGNYKARLKENNFDEIGALAIDFNKMTQTLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHEEEEEHHHHHHHHH
TSQEEIERQEKRRRQFIADVSHEMRTPLTTISGLTEGLVNDIIPKSETDRCIALIDTEAR
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECHHHH
RLTKLVNENLDYEKIRSNKIKLQKTRFNGKEFLELIKEQLDYVSSEKGNTITVSIDKDMA
HHHHHHHCCCCHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCEE
IYADYDRLTQVFINIVKNSVQFTENGQITLTGTQDYKESVLTITDTGIGMNTEELEQIWE
EEECHHHHHHHHHHHHHHCCEEECCCEEEEEECHHHHCCEEEEEECCCCCCHHHHHHHHH
RFYKADMSRTNTAFGESGIGLSIVKQLIEYHDGSITVTSEPNKGTSFTIRLPFFQDNEQ
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]