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The map label for this gene is pksG [H]

Identifier: 226224016

GI number: 226224016

Start: 1428887

End: 1430053

Strand: Direct

Name: pksG [H]

Synonym: Lm4b_01424

Alternate gene names: 226224016

Gene position: 1428887-1430053 (Clockwise)

Preceding gene: 226224013

Following gene: 226224022

Centisome position: 49.06

GC content: 37.45

Gene sequence:

>1167_bases
ATGAAAATTGGAATTGATAAAATAGGTTTTTATACTCCTGCATTTTATGTTGATATGACAGAACTTGCTGAAGCTAGAAA
TATTGATCCTAACAAATTTACTATTGGAATTGGCCAAGATAAAATGGCTTTTGCTCCGATTACGCAAGATTCAGTAACAA
TGGGCGCAAATGCAGCTTTACAGATTTTAGATGAAGAAGATTTGAAAAAAATTGATTTAGTTATTTTGGCGACAGAATCA
GGAATTGATGAGTCAAAGGCTGGCGCGGTTTATATTCACCGTTTGCTAGGTATTCAGCCGTTCTCTCGTGCAATTGAAAT
AAAAGAAGCTTGCTACGGAGCCACTGCAGGAATTAATTTAGCGAAAGATTATGTTGCGAAACATCCTGATAGTAAAGTAC
TGGTTATCGGATCGGATATTGCTCGTTACGGCTTGGCAACTGGCGGCGAAGCAACTCAAGGAGCTGGAGCTGTTGCGATG
GTTATTGCTGCTGATCCACGTTGTATCACACTTGAAGACGATAATGTGTTTTATACAGAAGATATTATGGACTTCTGGCG
CCCTGTTTACTCTGAGTATGCATGTGTAGAAGGTAAATATTCGACGGAGCAGTACATTCACTTTTTCCAAACCATTTGGG
AAAAGTATTCAGCAAAATTTGGCAAGAACTTAGAAGATTTTGCAGCTATTTGTTTCCACTTACCATATACAAAAATGGGT
AAAAAAGCGTTAGATACTATTATTGAAACAGCTCCAACTGAGGTTCAAGAAAGATTGCTAGAAAATTATCGTCTAAGTAC
GCTTTATAGCCGCAATGTCGGTAATATTTACACTGGTTCCCTTTATTTAAGCTTTATTTCCTTGTTAGACAATCAAGCAG
ATTTACAAGCAGAGGATAAAATTGGCTTCTTTAGTTACGGGTCTGGTGCTGTTGGCGAGTTCTTCCATGGAGTGCTACAA
CCTGACTATAAAAAATATATCCGTAAAGATGAACATGCGGAATTGTTAGCAAACCGTACGAAACTTGCTATTCCAGATTA
TGAAACGAAATTCAAACAACAATTACCTAAAGATGGTTCGACGTTTGAAGTGGATCCTGCTAGTGACCCAGCAACCATTG
TATTAACTGGTATTCAAGAGCATAAACGTCAATATATTAAAAAATAA

Upstream 100 bases:

>100_bases
TAAAAACTCACTAGATTAAGCCCAATGTATTGTATTTTATGCATTTTTCTACTAAAATTAAGGTACTTACAAATAAAATA
TAAAGGACTTGAATGTACAT

Downstream 100 bases:

>100_bases
TCAAAAAACCGACTAGGCTAAATCAGCTAGTCGGTTTTACTTTGAGCTCTTTATATTTTCTTGGGGATAAAAAACGAATC
ACAATGTAGCCAAGCATGAT

Product: hydroxy-3-methylglutaryl coenzyme A synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKIGIDKIGFYTPAFYVDMTELAEARNIDPNKFTIGIGQDKMAFAPITQDSVTMGANAALQILDEEDLKKIDLVILATES
GIDESKAGAVYIHRLLGIQPFSRAIEIKEACYGATAGINLAKDYVAKHPDSKVLVIGSDIARYGLATGGEATQGAGAVAM
VIAADPRCITLEDDNVFYTEDIMDFWRPVYSEYACVEGKYSTEQYIHFFQTIWEKYSAKFGKNLEDFAAICFHLPYTKMG
KKALDTIIETAPTEVQERLLENYRLSTLYSRNVGNIYTGSLYLSFISLLDNQADLQAEDKIGFFSYGSGAVGEFFHGVLQ
PDYKKYIRKDEHAELLANRTKLAIPDYETKFKQQLPKDGSTFEVDPASDPATIVLTGIQEHKRQYIKK

Sequences:

>Translated_388_residues
MKIGIDKIGFYTPAFYVDMTELAEARNIDPNKFTIGIGQDKMAFAPITQDSVTMGANAALQILDEEDLKKIDLVILATES
GIDESKAGAVYIHRLLGIQPFSRAIEIKEACYGATAGINLAKDYVAKHPDSKVLVIGSDIARYGLATGGEATQGAGAVAM
VIAADPRCITLEDDNVFYTEDIMDFWRPVYSEYACVEGKYSTEQYIHFFQTIWEKYSAKFGKNLEDFAAICFHLPYTKMG
KKALDTIIETAPTEVQERLLENYRLSTLYSRNVGNIYTGSLYLSFISLLDNQADLQAEDKIGFFSYGSGAVGEFFHGVLQ
PDYKKYIRKDEHAELLANRTKLAIPDYETKFKQQLPKDGSTFEVDPASDPATIVLTGIQEHKRQYIKK
>Mature_388_residues
MKIGIDKIGFYTPAFYVDMTELAEARNIDPNKFTIGIGQDKMAFAPITQDSVTMGANAALQILDEEDLKKIDLVILATES
GIDESKAGAVYIHRLLGIQPFSRAIEIKEACYGATAGINLAKDYVAKHPDSKVLVIGSDIARYGLATGGEATQGAGAVAM
VIAADPRCITLEDDNVFYTEDIMDFWRPVYSEYACVEGKYSTEQYIHFFQTIWEKYSAKFGKNLEDFAAICFHLPYTKMG
KKALDTIIETAPTEVQERLLENYRLSTLYSRNVGNIYTGSLYLSFISLLDNQADLQAEDKIGFFSYGSGAVGEFFHGVLQ
PDYKKYIRKDEHAELLANRTKLAIPDYETKFKQQLPKDGSTFEVDPASDPATIVLTGIQEHKRQYIKK

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=455, Percent_Identity=26.3736263736264, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI148298764, Length=370, Percent_Identity=28.1081081081081, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI148298677, Length=370, Percent_Identity=28.1081081081081, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17560126, Length=357, Percent_Identity=27.1708683473389, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6323509, Length=400, Percent_Identity=27.25, Blast_Score=116, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24654139, Length=448, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24654137, Length=448, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24654133, Length=448, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI24654135, Length=448, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI17933694, Length=448, Percent_Identity=25.8928571428571, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 43295; Mature: 43295

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGIDKIGFYTPAFYVDMTELAEARNIDPNKFTIGIGQDKMAFAPITQDSVTMGANAAL
CCCCCCCCCCCCCHHEEEHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCEECCCCCEE
QILDEEDLKKIDLVILATESGIDESKAGAVYIHRLLGIQPFSRAIEIKEACYGATAGINL
EECCHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
AKDYVAKHPDSKVLVIGSDIARYGLATGGEATQGAGAVAMVIAADPRCITLEDDNVFYTE
HHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEHH
DIMDFWRPVYSEYACVEGKYSTEQYIHFFQTIWEKYSAKFGKNLEDFAAICFHLPYTKMG
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHH
KKALDTIIETAPTEVQERLLENYRLSTLYSRNVGNIYTGSLYLSFISLLDNQADLQAEDK
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
IGFFSYGSGAVGEFFHGVLQPDYKKYIRKDEHAELLANRTKLAIPDYETKFKQQLPKDGS
CCEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCEEECCCHHHHHHHHCCCCCC
TFEVDPASDPATIVLTGIQEHKRQYIKK
EEEECCCCCCCEEEEECHHHHHHHHHCC
>Mature Secondary Structure
MKIGIDKIGFYTPAFYVDMTELAEARNIDPNKFTIGIGQDKMAFAPITQDSVTMGANAAL
CCCCCCCCCCCCCHHEEEHHHHHHHCCCCCCEEEEEECCCCEEECCCCCCCEECCCCCEE
QILDEEDLKKIDLVILATESGIDESKAGAVYIHRLLGIQPFSRAIEIKEACYGATAGINL
EECCHHCCCEEEEEEEEECCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHH
AKDYVAKHPDSKVLVIGSDIARYGLATGGEATQGAGAVAMVIAADPRCITLEDDNVFYTE
HHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCCCEEEEEEECCCEEEEEECCCEEEHH
DIMDFWRPVYSEYACVEGKYSTEQYIHFFQTIWEKYSAKFGKNLEDFAAICFHLPYTKMG
HHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHH
KKALDTIIETAPTEVQERLLENYRLSTLYSRNVGNIYTGSLYLSFISLLDNQADLQAEDK
HHHHHHHHHCCCHHHHHHHHHHCCHHHHHCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCC
IGFFSYGSGAVGEFFHGVLQPDYKKYIRKDEHAELLANRTKLAIPDYETKFKQQLPKDGS
CCEEECCCCCHHHHHHHHCCHHHHHHHCCCHHHHHHHCCCEEECCCHHHHHHHHCCCCCC
TFEVDPASDPATIVLTGIQEHKRQYIKK
EEEECCCCCCCEEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]