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The map label for this gene is recA

Identifier: 226223999

GI number: 226223999

Start: 1409122

End: 1410168

Strand: Direct

Name: recA

Synonym: Lm4b_01407

Alternate gene names: 226223999

Gene position: 1409122-1410168 (Clockwise)

Preceding gene: 226223998

Following gene: 226224000

Centisome position: 48.38

GC content: 39.16

Gene sequence:

>1047_bases
GTGAATGATCGTCAAGCGGCATTAGACCAAGCTTTAAAACAAATTGAAAAACAATTCGGTAAAGGTTCCATTATGAAATT
AGGGGAACATTCAGACCAAAATATATCTACTATTTCTAGTGGCTCATTAGCATTAGATATTGCTTTAGGAGTCGGCGGAT
ATCCACGTGGACGTATTATTGAAGTATACGGGCCAGAGAGTTCCGGTAAAACAACTGTTGCACTTCATGCTATTGCGGAA
GTACAAGCACAAGGCGGAACAGCTGCATTTATCGATGCAGAACACGCACTAGATCCAGCTTATGCTAAAAACCTAGGTGT
AAATATTGATGAATTATTACTATCTCAACCTGATACAGGAGAGCAAGCATTAGAAATTGCAGAAGCATTAGTTAGAAGTG
GTGCTGTAGATATGCTAGTAATTGACTCCGTTGCAGCACTTGTACCACGTGCTGAAATCGAAGGGGAGATGGGTGATGCA
CACGTTGGGCTACAAGCACGTTTAATGTCCCAAGCATTGCGTAAACTTTCCGGTGTTATTAATAAATCAAAAACCATTGC
TATTTTCATTAACCAAATTCGTGAAAAAGTTGGTGTTATGTTTGGTAACCCAGAGATTACACCTGGTGGTCGTGCGCTTA
AATTCTATTCGACTGTACGTTTAGAAGTAAGACGTGCAGAACAACTTAAGCAAGGTACAGATGTAATGGGTAACAAAACA
AAAATTAAAGTTGTAAAAAACAAAGTAGCTCCTCCATTCCGTATTGCTGAAGTGGATATCATGTACGGTGAAGGTATTTC
ACGTGAAGGCGAGCTTGTTGATATGGCCGCTGAAGTGGATGTAATCAATAAGAGTGGTTCATGGTATTCTTACAAAGAAG
AACGTATCGGCCAAGGTCGTGAAAACGCAAAACAATATCTAAAAGAACATACAGATATTCGTGATGAGATTTCAAAACGT
GTTCGTGAAGAGTACGAAATCGACGGAAGCAATAAAGAACCTTTAGATGAAGGCGAAGAAACATTAAGTTTACTAGATGA
TGAATAA

Upstream 100 bases:

>100_bases
AAATGCGTTATAGTAATTTTAGGAAAACATTCTGACTGTTTTTTGAGAGAGAAGAAGTAGAAAAACAGCTATTAGGATAT
TTATTGAAGGAGGCAACAAT

Downstream 100 bases:

>100_bases
ATAATACTAAATACGTGATAGGAATTTACTTCCTGTCACGTATTTTTTACTATTTAAAAAAAACTAAGGAAAATAACCGT
ACATTTCATTCCTTTTCTTG

Product: recombinase A

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MNDRQAALDQALKQIEKQFGKGSIMKLGEHSDQNISTISSGSLALDIALGVGGYPRGRIIEVYGPESSGKTTVALHAIAE
VQAQGGTAAFIDAEHALDPAYAKNLGVNIDELLLSQPDTGEQALEIAEALVRSGAVDMLVIDSVAALVPRAEIEGEMGDA
HVGLQARLMSQALRKLSGVINKSKTIAIFINQIREKVGVMFGNPEITPGGRALKFYSTVRLEVRRAEQLKQGTDVMGNKT
KIKVVKNKVAPPFRIAEVDIMYGEGISREGELVDMAAEVDVINKSGSWYSYKEERIGQGRENAKQYLKEHTDIRDEISKR
VREEYEIDGSNKEPLDEGEETLSLLDDE

Sequences:

>Translated_348_residues
MNDRQAALDQALKQIEKQFGKGSIMKLGEHSDQNISTISSGSLALDIALGVGGYPRGRIIEVYGPESSGKTTVALHAIAE
VQAQGGTAAFIDAEHALDPAYAKNLGVNIDELLLSQPDTGEQALEIAEALVRSGAVDMLVIDSVAALVPRAEIEGEMGDA
HVGLQARLMSQALRKLSGVINKSKTIAIFINQIREKVGVMFGNPEITPGGRALKFYSTVRLEVRRAEQLKQGTDVMGNKT
KIKVVKNKVAPPFRIAEVDIMYGEGISREGELVDMAAEVDVINKSGSWYSYKEERIGQGRENAKQYLKEHTDIRDEISKR
VREEYEIDGSNKEPLDEGEETLSLLDDE
>Mature_348_residues
MNDRQAALDQALKQIEKQFGKGSIMKLGEHSDQNISTISSGSLALDIALGVGGYPRGRIIEVYGPESSGKTTVALHAIAE
VQAQGGTAAFIDAEHALDPAYAKNLGVNIDELLLSQPDTGEQALEIAEALVRSGAVDMLVIDSVAALVPRAEIEGEMGDA
HVGLQARLMSQALRKLSGVINKSKTIAIFINQIREKVGVMFGNPEITPGGRALKFYSTVRLEVRRAEQLKQGTDVMGNKT
KIKVVKNKVAPPFRIAEVDIMYGEGISREGELVDMAAEVDVINKSGSWYSYKEERIGQGRENAKQYLKEHTDIRDEISKR
VREEYEIDGSNKEPLDEGEETLSLLDDE

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=321, Percent_Identity=63.8629283489097, Blast_Score=433, Evalue=1e-123,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37951; Mature: 37951

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRQAALDQALKQIEKQFGKGSIMKLGEHSDQNISTISSGSLALDIALGVGGYPRGRII
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCEE
EVYGPESSGKTTVALHAIAEVQAQGGTAAFIDAEHALDPAYAKNLGVNIDELLLSQPDTG
EEECCCCCCCEEEEEEEHHHHHCCCCEEEEEEHHHHCCHHHHHHCCCCHHHHHCCCCCCH
EQALEIAEALVRSGAVDMLVIDSVAALVPRAEIEGEMGDAHVGLQARLMSQALRKLSGVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NKSKTIAIFINQIREKVGVMFGNPEITPGGRALKFYSTVRLEVRRAEQLKQGTDVMGNKT
CCCCEEEHHHHHHHHHHCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCE
KIKVVKNKVAPPFRIAEVDIMYGEGISREGELVDMAAEVDVINKSGSWYSYKEERIGQGR
EEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHEEEECCCCCCCCCHHHHHCCCH
ENAKQYLKEHTDIRDEISKRVREEYEIDGSNKEPLDEGEETLSLLDDE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MNDRQAALDQALKQIEKQFGKGSIMKLGEHSDQNISTISSGSLALDIALGVGGYPRGRII
CCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCEEECCCCEEEEEEECCCCCCCCCEE
EVYGPESSGKTTVALHAIAEVQAQGGTAAFIDAEHALDPAYAKNLGVNIDELLLSQPDTG
EEECCCCCCCEEEEEEEHHHHHCCCCEEEEEEHHHHCCHHHHHHCCCCHHHHHCCCCCCH
EQALEIAEALVRSGAVDMLVIDSVAALVPRAEIEGEMGDAHVGLQARLMSQALRKLSGVI
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
NKSKTIAIFINQIREKVGVMFGNPEITPGGRALKFYSTVRLEVRRAEQLKQGTDVMGNKT
CCCCEEEHHHHHHHHHHCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCE
KIKVVKNKVAPPFRIAEVDIMYGEGISREGELVDMAAEVDVINKSGSWYSYKEERIGQGR
EEEEEECCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHEEEECCCCCCCCCHHHHHCCCH
ENAKQYLKEHTDIRDEISKRVREEYEIDGSNKEPLDEGEETLSLLDDE
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]