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The map label for this gene is buk [H]

Identifier: 226223971

GI number: 226223971

Start: 1377225

End: 1378292

Strand: Direct

Name: buk [H]

Synonym: Lm4b_01379

Alternate gene names: 226223971

Gene position: 1377225-1378292 (Clockwise)

Preceding gene: 226223970

Following gene: 226223972

Centisome position: 47.28

GC content: 39.89

Gene sequence:

>1068_bases
ATGTCTTTTGATGTTTTAACCATAAATCCTGGTTCCACGTCTACCAAACTCGCTGTTTACCAAGGCGATAAAGTACTTTT
TGAAGAAACTGTCAGACATACAATGCAAGAACTAGCCGACTTTAATAACGTACAGGAACAATTTGATTTTAGGTGGCAAG
TTCTACGTCGAGTGATGGATGCACATGGTTATGATGTAAAGAAATTACAAGCGGTAGTTGGAAGAGGGGGCTTACTTCGA
CCAGTTGCCGGCGGTACATATATGGTTACAGAAAAAATGATAGATGATTTAAAAGAAAATAAATACGGAGAGCATGCCTC
AAATTTAGGGGCACTGCTTGCAAAAAAATTAGCAGATGAACTGACAATACCTAGTTTTATTGTGGATCCGGTTGTCGTGG
ATGAAATGCAGGAAATTGCAAGGATATCTGGCAATGCCTTCATTGCTAGAAAAAGTATTTTTCATGCATTAAATCATAAA
GCAGCAGGTCGGAAAATAGCCAAAGAGCTAGGAAAAGATTATGAAAAAATGAATTTTGTTATCGCGCATCTTGGCGGTGG
AATTTCAGTTGCCGCTCATCGACAAGGAAAAGCGGTGGATGTGAATAATGCCTTAGATGGGGACGGACCTTTTAGTCCGG
AACGTTCTGGTTCTTTACCGATGAATGATTTTCTAGAAGCATGTTTTAGCGGTAAATGGACAAAACGCGAATTGCATGAA
CTGATTGTTGGTCGTGGTGGGATGATTTCATATTTAGGAACTAACAGCATGCTCGAGGTGGAAGCTAAAGTACAAGCAGG
TGACGTGAAAGCTATCGAAGCTTTTGATGCAATGGCTTATCAAGTTAGCAAAGAAATTGGCGCATGTTCCGTTGTATTGC
AAGGTAAAGTTGACGCCATAATTTTAACTGGTGGCCTTGCTAGAAGTGAACTTTTTACAAACAAAATTATTGAGCAAACA
AACTGGATAACCAGTGTGATTATAGAACCCGGAGAAGACGAATTAGAAGCATTAAATAGCGGTGTGCAACGTGTGCTCGC
TGGTCTTGAGAAAGAAAAAGAGTACTAA

Upstream 100 bases:

>100_bases
ATGCAAAGGCTTTTATTTGATTACAGCCTACGTGTTACAATAGTCCATGGGAAAATGATAACCTACCGCATTTTTCACAA
TTAAATTTGGAGGAAAGCAA

Downstream 100 bases:

>100_bases
AAGGAGGAGATAATGTGGCAACAGAATATGATGTCGTTATTCTTGGCGGAGGAACTGGCGGTTACGTCGCAGCAATTCAA
GCAGCTAAGAATGGCCAGAA

Product: butyrate kinase

Products: NA

Alternate protein names: BK; Branched-chain carboxylic acid kinase [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQELADFNNVQEQFDFRWQVLRRVMDAHGYDVKKLQAVVGRGGLLR
PVAGGTYMVTEKMIDDLKENKYGEHASNLGALLAKKLADELTIPSFIVDPVVVDEMQEIARISGNAFIARKSIFHALNHK
AAGRKIAKELGKDYEKMNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWTKRELHE
LIVGRGGMISYLGTNSMLEVEAKVQAGDVKAIEAFDAMAYQVSKEIGACSVVLQGKVDAIILTGGLARSELFTNKIIEQT
NWITSVIIEPGEDELEALNSGVQRVLAGLEKEKEY

Sequences:

>Translated_355_residues
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQELADFNNVQEQFDFRWQVLRRVMDAHGYDVKKLQAVVGRGGLLR
PVAGGTYMVTEKMIDDLKENKYGEHASNLGALLAKKLADELTIPSFIVDPVVVDEMQEIARISGNAFIARKSIFHALNHK
AAGRKIAKELGKDYEKMNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWTKRELHE
LIVGRGGMISYLGTNSMLEVEAKVQAGDVKAIEAFDAMAYQVSKEIGACSVVLQGKVDAIILTGGLARSELFTNKIIEQT
NWITSVIIEPGEDELEALNSGVQRVLAGLEKEKEY
>Mature_354_residues
SFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQELADFNNVQEQFDFRWQVLRRVMDAHGYDVKKLQAVVGRGGLLRP
VAGGTYMVTEKMIDDLKENKYGEHASNLGALLAKKLADELTIPSFIVDPVVVDEMQEIARISGNAFIARKSIFHALNHKA
AGRKIAKELGKDYEKMNFVIAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWTKRELHEL
IVGRGGMISYLGTNSMLEVEAKVQAGDVKAIEAFDAMAYQVSKEIGACSVVLQGKVDAIILTGGLARSELFTNKIIEQTN
WITSVIIEPGEDELEALNSGVQRVLAGLEKEKEY

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

None

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 38919; Mature: 38788

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQELADFNNVQEQFDFRWQVLRRVMD
CCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHGYDVKKLQAVVGRGGLLRPVAGGTYMVTEKMIDDLKENKYGEHASNLGALLAKKLADE
CCCCCHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LTIPSFIVDPVVVDEMQEIARISGNAFIARKSIFHALNHKAAGRKIAKELGKDYEKMNFV
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEE
IAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWTKRELHE
HEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
LIVGRGGMISYLGTNSMLEVEAKVQAGDVKAIEAFDAMAYQVSKEIGACSVVLQGKVDAI
HHHCCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
ILTGGLARSELFTNKIIEQTNWITSVIIEPGEDELEALNSGVQRVLAGLEKEKEY
EEECCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SFDVLTINPGSTSTKLAVYQGDKVLFEETVRHTMQELADFNNVQEQFDFRWQVLRRVMD
CEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHGYDVKKLQAVVGRGGLLRPVAGGTYMVTEKMIDDLKENKYGEHASNLGALLAKKLADE
CCCCCHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
LTIPSFIVDPVVVDEMQEIARISGNAFIARKSIFHALNHKAAGRKIAKELGKDYEKMNFV
CCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEE
IAHLGGGISVAAHRQGKAVDVNNALDGDGPFSPERSGSLPMNDFLEACFSGKWTKRELHE
HEECCCCEEEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHH
LIVGRGGMISYLGTNSMLEVEAKVQAGDVKAIEAFDAMAYQVSKEIGACSVVLQGKVDAI
HHHCCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEE
ILTGGLARSELFTNKIIEQTNWITSVIIEPGEDELEALNSGVQRVLAGLEKEKEY
EEECCCHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]