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The map label for this gene is recN

Identifier: 226223969

GI number: 226223969

Start: 1374401

End: 1376092

Strand: Direct

Name: recN

Synonym: Lm4b_01377

Alternate gene names: 226223969

Gene position: 1374401-1376092 (Clockwise)

Preceding gene: 226223968

Following gene: 226223970

Centisome position: 47.19

GC content: 38.59

Gene sequence:

>1692_bases
TTGCTTCAAGAAATGACAATTAAAAACTTTGCTATCATCGAATCGCTTTCTTTAACTTTTCAAGAAGGGATGACGGTACT
TACGGGGGAGACCGGCGCTGGTAAATCAATTATTATTGATGCGCTTGGTCTTCTTGTTGGCGGACGCGGGTCAGCTGATT
TTATCCGTCATGGAGAAGAACGTTTAGAACTTCAAGGGCTTTTTGCGCTAGCAGAGGATAACCTTGCTTGCCGAAATGCG
TTAATTGAAAATGGGATAGATGCTTCGGATGATATGGTTGTACTAGAACGCAGCCTGTTTCGCTCGGGGAAAAATAGTTG
CCGAATTAATGGCAAACTTGTAACTACCGTTCTCTTGCGCCAAATTGGCTCGAAATTAATAGATATCCATAGTCAGCATG
AACATCAGGAATTGATGAATGAGGAGTTTCATTTGTCATTACTTGATCGTTTCGCTTCTGACAAAATCAAGCCAGCACTA
ACCAAGTACCAAACTAATTTCAAAGAATATCAAACAATCGAGAAAGAGTGGCAAAATTGGACGAAAAATGAGCGGGAATT
AGCGCAACGTCTGGATATGCTCCGTTTTCAACAACAGGAAATTGAAAATGCCAATTTGCAAGCTGGTGAAGAAGATCGCT
TGTTAGAACAAAAAAATATCTTAGCCAATTTTGAAAAACTTAACGAAAATTTACAAGGAGCTTATGCCGCGATTCAAGGT
GAGCCTGGTGGGCTGGAATTTGTTGGAGAAGCAATGCGTCAAATGGAGGCAGCGGCGAGTATTCATACGGATTATAAAGC
GGTTAGTGAGGCCATTTCTTCTAGTTACTATATGTTAGAAGATAGCATGAGCCAAATTAGGCAATCACTAGATCAGCTCG
AATTCCAACCAGAAGAACTGAATCAAATTGAATCACGTTTGAATGATTTAAACCAATTAAAACGCAAATATGGTAAAACA
ATTGAAGATATCATTCAATACGAACAAGAAATAAGCAGCGAAATGGAAAAACTGACAGATAGTGAATCACATGTCGGGCA
TTTAGAGACCAAATTAGCAACACTTAAAGCAGAACTAACAAAACAAGCAAATACACTAACGGAAATCCGTAAAAAAGCGG
CTGTTACATTAGAAAAACAAATCAAACAAGAATTAAATCACTTATATATGGAGAAAGCCATTTTTAGTGTACGCTTCGAA
GCAAACAAAATGGAACTAACAGATTCAGGGCAAGATAGCGTCGTTTTCTATATGTCAACAAACCCGGGTGAACCTTTAAA
ACCATTAGCAAAAATCGCTTCTGGTGGAGAGTTATCTAGAATGATGTTAGCATTAAAAACCATTTTTTCTAGGCACCAAG
GTATCACCTCGATTATTTTCGATGAAGTAGATACTGGTGTAAGTGGTCGCGTTGGTCAAGCGATTGCAGAAAAAATTTAT
GCTGTTTCAGTTGGCTCGCAAGTACTATGCATTAGTCACTTACCTCAAGTTGCAGCAATGGCGAACCATCACTACTACAT
TACGAAAAAAGTCCAAAACAAACGGACAACAACTTCCGTTACAGTGCTTAAAGGGGAAGAGAAAGTAGAAGAAATCAGTC
GAATGATTGCTGGAATAGAAGTCACCGAATTAACCAAACAACATGCAAAAGAAATGATTGAACAAGCGGAAAAAGTAAAA
CAAACGTATTAA

Upstream 100 bases:

>100_bases
GATGACACTTGTTTAATTATTTGTCGCTCGGAAGAAAATGCGAAAACATTAACAGATCGTTTTATAGATATGCTTTAAAA
TTTGTTGGAGGTGAAAGATT

Downstream 100 bases:

>100_bases
AAAAACGAAATTGCTTGCATATCGAATAGTTTTTGCTTAAAATGAATAAGGTAGAAAATTATTACCTGGTCATAAAAACA
ACTACTAATTCGAAATGCGG

Product: DNA repair and genetic recombination protein RecN

Products: NA

Alternate protein names: Recombination protein N [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSADFIRHGEERLELQGLFALAEDNLACRNA
LIENGIDASDDMVVLERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEYQTIEKEWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYAAIQG
EPGGLEFVGEAMRQMEAAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPEELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISSEMEKLTDSESHVGHLETKLATLKAELTKQANTLTEIRKKAAVTLEKQIKQELNHLYMEKAIFSVRFE
ANKMELTDSGQDSVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTVLKGEEKVEEISRMIAGIEVTELTKQHAKEMIEQAEKVK
QTY

Sequences:

>Translated_563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSADFIRHGEERLELQGLFALAEDNLACRNA
LIENGIDASDDMVVLERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEYQTIEKEWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYAAIQG
EPGGLEFVGEAMRQMEAAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPEELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISSEMEKLTDSESHVGHLETKLATLKAELTKQANTLTEIRKKAAVTLEKQIKQELNHLYMEKAIFSVRFE
ANKMELTDSGQDSVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTVLKGEEKVEEISRMIAGIEVTELTKQHAKEMIEQAEKVK
QTY
>Mature_563_residues
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSADFIRHGEERLELQGLFALAEDNLACRNA
LIENGIDASDDMVVLERSLFRSGKNSCRINGKLVTTVLLRQIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPAL
TKYQTNFKEYQTIEKEWQNWTKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYAAIQG
EPGGLEFVGEAMRQMEAAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPEELNQIESRLNDLNQLKRKYGKT
IEDIIQYEQEISSEMEKLTDSESHVGHLETKLATLKAELTKQANTLTEIRKKAAVTLEKQIKQELNHLYMEKAIFSVRFE
ANKMELTDSGQDSVVFYMSTNPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTVLKGEEKVEEISRMIAGIEVTELTKQHAKEMIEQAEKVK
QTY

Specific function: Involved in recombinational repair of damaged DNA. Seems to be the first protein recruited to repair centers, foci that are the site of double-strand DNA break(s), followed by recO and then recF [H]

COG id: COG0497

COG function: function code L; ATPase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm, nucleoid. Note=Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recN family [H]

Homologues:

Organism=Escherichia coli, GI48994901, Length=571, Percent_Identity=33.0998248686515, Blast_Score=293, Evalue=2e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004604
- InterPro:   IPR003395 [H]

Pfam domain/function: PF02463 SMC_N [H]

EC number: NA

Molecular weight: Translated: 63519; Mature: 63519

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSADFIRHGEE
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHH
RLELQGLFALAEDNLACRNALIENGIDASDDMVVLERSLFRSGKNSCRINGKLVTTVLLR
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHH
QIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPALTKYQTNFKEYQTIEKEWQNW
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYAAIQG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECC
EPGGLEFVGEAMRQMEAAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPEEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHH
NQIESRLNDLNQLKRKYGKTIEDIIQYEQEISSEMEKLTDSESHVGHLETKLATLKAELT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KQANTLTEIRKKAAVTLEKQIKQELNHLYMEKAIFSVRFEANKMELTDSGQDSVVFYMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCCCCEEEEEEC
NPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTVLKGEEKVEEISRMIAGIE
HHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHCCH
VTELTKQHAKEMIEQAEKVKQTY
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLQEMTIKNFAIIESLSLTFQEGMTVLTGETGAGKSIIIDALGLLVGGRGSADFIRHGEE
CCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHCCHH
RLELQGLFALAEDNLACRNALIENGIDASDDMVVLERSLFRSGKNSCRINGKLVTTVLLR
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEECCHHHHHHHHH
QIGSKLIDIHSQHEHQELMNEEFHLSLLDRFASDKIKPALTKYQTNFKEYQTIEKEWQNW
HHHHHHHHHHCHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TKNERELAQRLDMLRFQQQEIENANLQAGEEDRLLEQKNILANFEKLNENLQGAYAAIQG
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECC
EPGGLEFVGEAMRQMEAAASIHTDYKAVSEAISSSYYMLEDSMSQIRQSLDQLEFQPEEL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHH
NQIESRLNDLNQLKRKYGKTIEDIIQYEQEISSEMEKLTDSESHVGHLETKLATLKAELT
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
KQANTLTEIRKKAAVTLEKQIKQELNHLYMEKAIFSVRFEANKMELTDSGQDSVVFYMST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCEEECCCCCCCEEEEEEC
NPGEPLKPLAKIASGGELSRMMLALKTIFSRHQGITSIIFDEVDTGVSGRVGQAIAEKIY
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AVSVGSQVLCISHLPQVAAMANHHYYITKKVQNKRTTTSVTVLKGEEKVEEISRMIAGIE
HHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEEECHHHHHHHHHHHHCCH
VTELTKQHAKEMIEQAEKVKQTY
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2106508; 8969508; 9384377; 2507400; 9044256 [H]