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The map label for this gene is folD

Identifier: 226223961

GI number: 226223961

Start: 1367196

End: 1368050

Strand: Direct

Name: folD

Synonym: Lm4b_01368

Alternate gene names: 226223961

Gene position: 1367196-1368050 (Clockwise)

Preceding gene: 226223960

Following gene: 226223962

Centisome position: 46.94

GC content: 38.71

Gene sequence:

>855_bases
ATGGGAGAAATTATTGATGGCAAAAAGTTAGCAAAAGAAATTCAAGAAAAAGTAACAAGAGAAGTAGCTGAATTAGTAAA
AGAAGGTAAGAAACCAGGGCTTGCTGTTGTGCTCGTTGGCGACAATCAAGCATCTCGTACATATGTAAGAAATAAACAAA
AACGGACAGAAGAAGCGGGGATGAAATCCGTTTTAATTGAACTTCCAGAAAATGTAACAGAAGAAAAATTACTATCTGTT
GTAGAGGAGCTTAACGAAGATAAAACTATTCATGGCATACTCGTGCAGTTACCACTACCAGAACACATTTCAGAAGAAAA
AGTAATTGATACTATTAGCTATGACAAAGATGTTGACGGTTTCCATCCAGTGAATGTAGGTAATTTATTCATCGGAAAAG
ATTCTTTTGTTCCTTGTACACCAGCAGGAATTATTGAACTTATAAAATCGACCGGCACTCAAATAGAAGGCAAACGCGCT
GTTGTTATTGGTAGAAGTAACATCGTAGGGAAACCAGTAGCTCAATTACTGTTAAATGAAAACGCGACAGTAACCATTGC
GCATAGCCGTACAAAAGATTTACCTCAAGTAGCGAAAGAAGCAGATATTCTTGTTGTAGCGACAGGCCTAGCGAAATTCG
TGAAAAAAGACTATATCAAACCAGGTGCGGTTGTTATTGATGTTGGCATGGATCGCGACGAAAATAATAAGTTATGCGGT
GATGTGGACTTTGATGATGTGGTAGAAGAAGCAGGATTTATCACGCCGGTACCAGGTGGCGTTGGCCCGATGACAATCAC
AATGCTACTTGCGAACACATTAAAAGCAGCAAAACGCATTTGGAAAATGAATTGA

Upstream 100 bases:

>100_bases
ATAAGCTTGATTGAGTGCAAGGGAAATCTCTTGCACTCCCTTATTTCGTGATTTTTTAAGAATAATTACGTGCAACTTAT
TTTTAAAGGGGGCAATACTC

Downstream 100 bases:

>100_bases
TTAATTGGATGTGAAATGATGGAGCAAGATAAATATTTGACGGTAGCAGCAATAACCAAATATATCGAAAAAAAGTTTGA
AGTAGATCCCTACATGAAGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MGEIIDGKKLAKEIQEKVTREVAELVKEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPENVTEEKLLSV
VEELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRA
VVIGRSNIVGKPVAQLLLNENATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMDRDENNKLCG
DVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN

Sequences:

>Translated_284_residues
MGEIIDGKKLAKEIQEKVTREVAELVKEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPENVTEEKLLSV
VEELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRA
VVIGRSNIVGKPVAQLLLNENATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMDRDENNKLCG
DVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
>Mature_283_residues
GEIIDGKKLAKEIQEKVTREVAELVKEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAGMKSVLIELPENVTEEKLLSVV
EELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDGFHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAV
VIGRSNIVGKPVAQLLLNENATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMDRDENNKLCGD
VDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=48.122866894198, Blast_Score=256, Evalue=2e-68,
Organism=Homo sapiens, GI222136639, Length=291, Percent_Identity=46.3917525773196, Blast_Score=253, Evalue=1e-67,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=46.2837837837838, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI36796743, Length=240, Percent_Identity=27.9166666666667, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786741, Length=277, Percent_Identity=49.4584837545126, Blast_Score=262, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=293, Percent_Identity=43.0034129692833, Blast_Score=209, Evalue=1e-54,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=46.3414634146341, Blast_Score=268, Evalue=5e-73,
Organism=Saccharomyces cerevisiae, GI6321643, Length=296, Percent_Identity=46.2837837837838, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6322933, Length=328, Percent_Identity=28.0487804878049, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=46.7128027681661, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=46.7128027681661, Blast_Score=259, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=46.7128027681661, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=46.7128027681661, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI17136816, Length=297, Percent_Identity=44.7811447811448, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI17136818, Length=297, Percent_Identity=44.7811447811448, Blast_Score=240, Evalue=6e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30910; Mature: 30779

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEIIDGKKLAKEIQEKVTREVAELVKEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
MKSVLIELPENVTEEKLLSVVEELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDG
HHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCHHHHHHHHCCCCCCCC
FHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAVVIGRSNIVGKPVAQLLLNE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEECCCCCHHHHHHHHHCC
NATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMDRDENNKLCG
CCEEEEECCCCCCHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
DVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
CCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GEIIDGKKLAKEIQEKVTREVAELVKEGKKPGLAVVLVGDNQASRTYVRNKQKRTEEAG
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
MKSVLIELPENVTEEKLLSVVEELNEDKTIHGILVQLPLPEHISEEKVIDTISYDKDVDG
HHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCHHCCHHHHHHHHCCCCCCCC
FHPVNVGNLFIGKDSFVPCTPAGIIELIKSTGTQIEGKRAVVIGRSNIVGKPVAQLLLNE
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCEECCCEEEEEECCCCCHHHHHHHHHCC
NATVTIAHSRTKDLPQVAKEADILVVATGLAKFVKKDYIKPGAVVIDVGMDRDENNKLCG
CCEEEEECCCCCCHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCC
DVDFDDVVEEAGFITPVPGGVGPMTITMLLANTLKAAKRIWKMN
CCCHHHHHHHCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]