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The map label for this gene is gltX

Identifier: 226222866

GI number: 226222866

Start: 253588

End: 255063

Strand: Direct

Name: gltX

Synonym: Lm4b_00257

Alternate gene names: 226222866

Gene position: 253588-255063 (Clockwise)

Preceding gene: 226222865

Following gene: 226222867

Centisome position: 8.71

GC content: 37.94

Gene sequence:

>1476_bases
GTGAGTGAAACAAAACGAGTACGTGTACGTTATGCACCAAGTCCAACTGGATTTTTGCATATTGGGAATGCGCGTACTGC
CCTATTTAACTATTTATTTGCAAGACATAATGATGGAGATTTTATCATTCGAATTGAAGACACGGATGCGAAACGTAACA
TAGCTGACGGTGAAGAAAGTCAGATGACAAATTTGAAATGGCTAGGTATGGACTGGGATGAAGGTGTGGATGTTCCTGGA
AAATACGGACCTTACCGTCAATCGGAACGTCAATCCATTTATGAACCGTTAATTCAACAACTTCTGGATGAAGGTTTGGC
TTATAAATGTTATTGTACGGAAGAAGAGCTGGATGCTGAACGTGAAAAACAAAAAGCAAACGGTGAAATGCCTCGTTATA
GCGGGAAATGTCGTCATTTAACAAAAGAACAACAAGCGGAGAAAGAAGCGCAAGGTTTTAAACCAAGTATTCGTTTCAAA
GTGCCAGCAAATGAAACAATCACTTTTAACGATATGGTGAAAGACGATGTTTCTTTTGAATCAAATGGAATAGGCGATTT
TGTTATTGCGAAAAAAGATGGAATTCCGACTTATAACTTTGCTGTAGCAGTGGATGACCATTTAATGGAAATCTCTCATG
TTCTTCGTGGGGATGACCATATTTCGAATACGCCTAAGCAAATTTTGATTTATAATGCATTTGGCTGGGAGCCGCCAATT
TTTGGTCATATGACACTTATTGTGAATGAGAGCAGACGTAAATTAAGTAAACGTGACGGTTCGATTATTCAATTTATCGA
ACAATATCGCGATTTAGGATATTTACCAGAAGCATTATTTAACTTTATTGCTATGCTTGGTTGGTCTCCAGAAGGGGAAG
AAGAAATTTTCTCTAAAGAAGAATTCATTAAAATGTTTGATCCGAAGCGTTTATCTAAATCACCAGCATTATTTGATAAT
GTTAAATTGACTTGGGTGAATAACCAATATGTGAAAAAATTACCACTAAATGATGTAGTAGAACTATCGCTACCGCATTT
ACAAAAAGCAGGTGTTGTATCTGCTGAGTTAGATCAAGCGGAACTTGATTGGGTGCACAAATTAGTGTCACTTTATCATG
AGCAAATGAGCTATGGTGCGGAAATTGTGCCACTTTCTGAAATGTTTTTTGCTGACGCAGAGTCCATTACGTTTGATGAA
GAAGAGACAGCAGTGCTTGCGGAAGAGACAGTTCCAACTGTTATTTCAGCATTCAAAAAAGAATTAGAAGCACTAGAAGT
TCTAGAAGCTGCGGAAGTAAAAGCGGCAATCAAGCGGGTTCAAAAAGAAACGGGCGTAAAAGGTAAAGGTTTATTTATGC
CAATTCGTATTGTGACAACGGGTGAAATGCATGGTCCTGAATTGCCACTTGCAATCGAAGTTCTTGGTCGTGAAAAAGTG
CTTAATCGTTTGGATACATGGTTGAAAAATAATTAA

Upstream 100 bases:

>100_bases
GGCGACTACGACAGAGAAATTAGGGTTTACTGGTCGGAAAGAAGGCATCGCAAGTCTAGCCGTTGTCTTACTTGAAAAAT
AATTGAAGGAGTGAATAATT

Downstream 100 bases:

>100_bases
TTTTGAATGAAAGCATCTCGGCTATTTGCGGGGTGCTTTTTTATTTTGATATATTTCAGTTGCAAGGAATTTTATTGCTG
TTATAATAGAACTAAATATA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNIADGEESQMTNLKWLGMDWDEGVDVPG
KYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELDAEREKQKANGEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFK
VPANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPI
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDN
VKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSAELDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAESITFDE
EETAVLAEETVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
LNRLDTWLKNN

Sequences:

>Translated_491_residues
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNIADGEESQMTNLKWLGMDWDEGVDVPG
KYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELDAEREKQKANGEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFK
VPANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPI
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDN
VKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSAELDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAESITFDE
EETAVLAEETVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
LNRLDTWLKNN
>Mature_490_residues
SETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNIADGEESQMTNLKWLGMDWDEGVDVPGK
YGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELDAEREKQKANGEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKV
PANETITFNDMVKDDVSFESNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPIF
GHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKEEFIKMFDPKRLSKSPALFDNV
KLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSAELDQAELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAESITFDEE
ETAVLAEETVPTVISAFKKELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKVL
NRLDTWLKNN

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=336, Percent_Identity=40.1785714285714, Blast_Score=259, Evalue=3e-69,
Organism=Homo sapiens, GI4826960, Length=304, Percent_Identity=24.3421052631579, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788743, Length=483, Percent_Identity=37.6811594202899, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI226510925, Length=262, Percent_Identity=33.206106870229, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786895, Length=214, Percent_Identity=27.1028037383178, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=484, Percent_Identity=34.297520661157, Blast_Score=253, Evalue=2e-67,
Organism=Caenorhabditis elegans, GI17511007, Length=260, Percent_Identity=23.8461538461538, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=507, Percent_Identity=34.1222879684418, Blast_Score=272, Evalue=8e-74,
Organism=Saccharomyces cerevisiae, GI6324742, Length=313, Percent_Identity=24.6006389776358, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=211, Percent_Identity=27.4881516587678, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=34.8697394789579, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI17864488, Length=304, Percent_Identity=25.9868421052632, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55979; Mature: 55848

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNIADGEES
CCCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHH
QMTNLKWLGMDWDEGVDVPGKYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELDAE
HCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
REKQKANGEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKVPANETITFNDMVKDDVSFE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCC
SNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPI
CCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKE
CEEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EFIKMFDPKRLSKSPALFDNVKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSAELDQA
HHHHHCCCHHCCCCCCEECCEEEEEECCHHHHHCCCCHHHHCCCCCHHHCCCEEHHCCHH
ELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAESITFDEEETAVLAEETVPTVISAFKK
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
ELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHHH
LNRLDTWLKNN
HHHHHHHHCCC
>Mature Secondary Structure 
SETKRVRVRYAPSPTGFLHIGNARTALFNYLFARHNDGDFIIRIEDTDAKRNIADGEES
CCCCEEEEEECCCCCCEEEECCHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCHH
QMTNLKWLGMDWDEGVDVPGKYGPYRQSERQSIYEPLIQQLLDEGLAYKCYCTEEELDAE
HCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHH
REKQKANGEMPRYSGKCRHLTKEQQAEKEAQGFKPSIRFKVPANETITFNDMVKDDVSFE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEHHHHHCCCCCC
SNGIGDFVIAKKDGIPTYNFAVAVDDHLMEISHVLRGDDHISNTPKQILIYNAFGWEPPI
CCCCCCEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
FGHMTLIVNESRRKLSKRDGSIIQFIEQYRDLGYLPEALFNFIAMLGWSPEGEEEIFSKE
CEEEEEEEECHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHH
EFIKMFDPKRLSKSPALFDNVKLTWVNNQYVKKLPLNDVVELSLPHLQKAGVVSAELDQA
HHHHHCCCHHCCCCCCEECCEEEEEECCHHHHHCCCCHHHHCCCCCHHHCCCEEHHCCHH
ELDWVHKLVSLYHEQMSYGAEIVPLSEMFFADAESITFDEEETAVLAEETVPTVISAFKK
HHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
ELEALEVLEAAEVKAAIKRVQKETGVKGKGLFMPIRIVTTGEMHGPELPLAIEVLGREKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCCCCCEEEEHHHHHHH
LNRLDTWLKNN
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]