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The map label for this gene is pth

Identifier: 226222842

GI number: 226222842

Start: 213715

End: 214275

Strand: Direct

Name: pth

Synonym: Lm4b_00211

Alternate gene names: 226222842

Gene position: 213715-214275 (Clockwise)

Preceding gene: 226222841

Following gene: 226222843

Centisome position: 7.34

GC content: 38.5

Gene sequence:

>561_bases
ATGAAACTAATCGCAGGACTCGGAAACCCGGGCAAAAAATACGAACGTACCCGGCATAATGTCGGTTTTATGGTAGTTGA
CGAACTAAGTTTTCGTCATCAAACCCCTTGGAAGAAATCAAAATTTAATGGTATGACTAGCGAAATTATCGTCGGCGGTG
AAAAAATGATTTTAGTGAAACCACTCACTTTTATGAACGCTTCAGGTGAATGCATTCGTCCGCTAATGGATTACTACAAC
ATACCAATTGAAGATGTCGTGATTGTGTATGATGATCTCGATTTACCAGTTGGGAAGATTAGATTACGCCAAAAAGGTAG
TGCTGGCGGACATAATGGAATGAAATCAATTATTCAACACGTTAAAACACAAGAGTTTAATCGCATTCGTGTTGGCGTTA
GTCGTCCTTTAAAAGGAGAAGTTATTCATTATGTCTTGGGAGATTTTCCTAAAGCAGAACAACCTGATATTATCGCAGCG
ATTCAAAAAAGCGCTGACGCCATCGAAGACTTTGCACAGACACCATTTATAGAAGTTATGAACAAATACAATCAAAAATA
G

Upstream 100 bases:

>100_bases
AACTTTCAACCAAGTGAAATCCATGTTATAATCAAAAAAACATCGAAACAAAAACCAGTTCATTCGTGAAGTGGTTTCGT
TCATGTAAGGAGAAGCTTTT

Downstream 100 bases:

>100_bases
ACGATGACGAGGCGTGGCAATCTTTTGCCACGTATTTCGGTCTTTCTTCGGACGCGTGATTTACTCTTGCGTTGCATAGA
ATGAATTTTCTGACAAAAAG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMTSEIIVGGEKMILVKPLTFMNASGECIRPLMDYYN
IPIEDVVIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHVKTQEFNRIRVGVSRPLKGEVIHYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQTPFIEVMNKYNQK

Sequences:

>Translated_186_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMTSEIIVGGEKMILVKPLTFMNASGECIRPLMDYYN
IPIEDVVIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHVKTQEFNRIRVGVSRPLKGEVIHYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQTPFIEVMNKYNQK
>Mature_186_residues
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMTSEIIVGGEKMILVKPLTFMNASGECIRPLMDYYN
IPIEDVVIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQHVKTQEFNRIRVGVSRPLKGEVIHYVLGDFPKAEQPDIIAA
IQKSADAIEDFAQTPFIEVMNKYNQK

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=172, Percent_Identity=33.7209302325581, Blast_Score=107, Evalue=7e-24,
Organism=Escherichia coli, GI1787455, Length=187, Percent_Identity=37.4331550802139, Blast_Score=114, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_LISMC (C1KYE6)

Other databases:

- EMBL:   FM242711
- RefSeq:   YP_002756949.1
- ProteinModelPortal:   C1KYE6
- SMR:   C1KYE6
- GeneID:   7703646
- GenomeReviews:   FM242711_GR
- KEGG:   lmc:Lm4b_00211
- OMA:   SHAGHNG
- ProtClustDB:   PRK05426
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21003; Mature: 21003

Theoretical pI: Translated: 9.73; Mature: 9.73

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMTSEIIVGGEKMILVK
CCEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCHHEEECCCEEEEEC
PLTFMNASGECIRPLMDYYNIPIEDVVIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQH
CHHHCCCCCHHHHHHHHHHCCCHHHEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
VKTQEFNRIRVGVSRPLKGEVIHYVLGDFPKAEQPDIIAAIQKSADAIEDFAQTPFIEVM
HHHHCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
NKYNQK
HHHCCC
>Mature Secondary Structure
MKLIAGLGNPGKKYERTRHNVGFMVVDELSFRHQTPWKKSKFNGMTSEIIVGGEKMILVK
CCEECCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCCCHHHCCCCCHHEEECCCEEEEEC
PLTFMNASGECIRPLMDYYNIPIEDVVIVYDDLDLPVGKIRLRQKGSAGGHNGMKSIIQH
CHHHCCCCCHHHHHHHHHHCCCHHHEEEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHH
VKTQEFNRIRVGVSRPLKGEVIHYVLGDFPKAEQPDIIAAIQKSADAIEDFAQTPFIEVM
HHHHCCCEEEECCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
NKYNQK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA