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The map label for this gene is appA [H]

Identifier: 226222774

GI number: 226222774

Start: 140028

End: 141602

Strand: Direct

Name: appA [H]

Synonym: Lm4b_00139

Alternate gene names: 226222774

Gene position: 140028-141602 (Clockwise)

Preceding gene: 226222773

Following gene: 226222775

Centisome position: 4.81

GC content: 39.11

Gene sequence:

>1575_bases
ATGAAGAAATTTTTATTAGTAGCGGTTATCTCGGTTTTTGCCTTGGTGTTAACGGCTTGCGGAGGCTCTGGCGCTAGTTC
AGACAAAGCAAATGGTTCAGGCAAAGCGAAAGACGGCGGCTCTCTAATTATCGGCGTTACTGGGGATCCAGAAGTTATCA
ATCCGAACTACGCTTCTGACCGTGTAACATTAACGATTCAACAAGCTGTTTACGCACCGCTATTTTGGGAAGTTGACGGG
AAACCGGCACTTGCAAAAAGCTTAGATATTTCAGATGACAACTTAACTTACACTGTGAAACTAAAAGATGGTTTAACTTG
GCACGACGGCAAACCCTTAACTGCAGACGATGTAGTATTTACTGTAAATTCTATTTTAGATACAAAACAAAACAGCCCGA
ACCGCGGCAACTTTGTTTTTGACGATAAACCTGTAAAAGTAGAAGCAGTAGACGACACAACGGTGAAATTCACTTTACCA
ACTGTTGCTCCAGCCTTTGAAAATACAATTAAAACTTTCTTCCCAATTCCAAAACACATTTTTGAAGGGGTAGAAAACAT
CGAGAAAAGTGATAAAAACAAAAATCCAATCGGCTCAGGTCCGTACAAATTTGTTGAATACAAAACAGGCGAATATGTTT
CCCTAGAACGATTCAACGACTATTTTGACGGCAAACCAAAATTGGATAAAGTTACTTTCCGAATCACAAAAGACCAAAAC
GCTGCTAATTTAGCACTGCAAAATGGCGAAATCAACCTAAAATCCATTCAACCTTCTGATAGAAACAAAGTAGAAAAAGC
GAGCGCGGTAAACATTATCACTTACCCGGAAAATCGCTTAAGCTATGCAACATTCAACGAAAACCAACCAGCACTTAAAT
CAAAAGAACTTCGCCAAGCCCTTTCTTATGCGCTTGATCGTGAAGAAATCATTGATGCTGCATACGGTTCAGATGAATAC
GCAAAACCAGCTTCCTCGTTCCTAACAGAAAACACAAAATATTTCACGGATAAAGTAGAAACATACGACCAAGACATTGC
CAAAGCGAAAAAATTAGTAAAAGAAAGTGGTTTTGATACAAGTCAAAAACTAACTGTTTACTATTTAAACAACAGTAAAT
CACAAGAAAGTATCGCGCTTTACTTGCAACAACAATACAAAGAAATCGGCGTGACACTTGACTTGAAACCAACAGATCCA
AATGCACTAAGCAACATCACACTTGACCGCAAAAACGCAGACTACTCTATCGCACTAAATGGTTATATCATGGGGAACGA
TCCTGATGCGTATAAATCCCTATACCTAAGCGATGCGCCATATAACTATTCGAACTACCACAACAAAGATCTAGATGCAC
TTTGGGAAAAAGGTGCTGTAACTGCAGACGATAAAGAACGTCAAGAAATCTATGAAAAAATCCAAAACACCATTGCTGAT
GACGCTGTAATTTATCCAATTTCTTATGATAATGCAGTGCTAGCTCTTGATAGCCGTTACGGTGGTCAAAAAGCTGCCAC
ACCACAACCAGTAACCATGTTCCGTGATCTTTCTAAACTATATTTAACGGAATAA

Upstream 100 bases:

>100_bases
CTGGCAGATAAGTAGTAGCTTTTAATGAGGCGCTTCGATTCTGACCAAAAAACAGAGGAAGTGTTATTTTTTAGCGCTTA
AAGAGGGGAGTTTTTGTTAG

Downstream 100 bases:

>100_bases
TAATTAAAAATCATTCGTAAAAAATCTGGCCTGCCATGCTTGTTTTTGGCAGGTTCAGGTTTTTACTTGTCATTTTTAGT
GTTTTAGATAGGAGATAAAT

Product: oligopeptide ABC transport system substrate-binding proteins

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MKKFLLVAVISVFALVLTACGGSGASSDKANGSGKAKDGGSLIIGVTGDPEVINPNYASDRVTLTIQQAVYAPLFWEVDG
KPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVFTVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLP
TVAPAFENTIKTFFPIPKHIFEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQN
AANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQALSYALDREEIIDAAYGSDEY
AKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDTSQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDP
NALSNITLDRKNADYSIALNGYIMGNDPDAYKSLYLSDAPYNYSNYHNKDLDALWEKGAVTADDKERQEIYEKIQNTIAD
DAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE

Sequences:

>Translated_524_residues
MKKFLLVAVISVFALVLTACGGSGASSDKANGSGKAKDGGSLIIGVTGDPEVINPNYASDRVTLTIQQAVYAPLFWEVDG
KPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVFTVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLP
TVAPAFENTIKTFFPIPKHIFEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQN
AANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQALSYALDREEIIDAAYGSDEY
AKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDTSQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDP
NALSNITLDRKNADYSIALNGYIMGNDPDAYKSLYLSDAPYNYSNYHNKDLDALWEKGAVTADDKERQEIYEKIQNTIAD
DAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE
>Mature_524_residues
MKKFLLVAVISVFALVLTACGGSGASSDKANGSGKAKDGGSLIIGVTGDPEVINPNYASDRVTLTIQQAVYAPLFWEVDG
KPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVFTVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLP
TVAPAFENTIKTFFPIPKHIFEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQN
AANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQALSYALDREEIIDAAYGSDEY
AKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDTSQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDP
NALSNITLDRKNADYSIALNGYIMGNDPDAYKSLYLSDAPYNYSNYHNKDLDALWEKGAVTADDKERQEIYEKIQNTIAD
DAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=486, Percent_Identity=25.9259259259259, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1789966, Length=481, Percent_Identity=25.5717255717256, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787762, Length=481, Percent_Identity=26.6112266112266, Blast_Score=132, Evalue=7e-32,
Organism=Escherichia coli, GI1789397, Length=493, Percent_Identity=24.1379310344828, Blast_Score=126, Evalue=4e-30,
Organism=Escherichia coli, GI87081878, Length=433, Percent_Identity=27.7136258660508, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787551, Length=517, Percent_Identity=22.4371373307544, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1789887, Length=480, Percent_Identity=23.75, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787495, Length=476, Percent_Identity=25, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87082063, Length=390, Percent_Identity=23.0769230769231, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 58287; Mature: 58287

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFLLVAVISVFALVLTACGGSGASSDKANGSGKAKDGGSLIIGVTGDPEVINPNYASD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCC
RVTLTIQQAVYAPLFWEVDGKPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVF
EEEEEEHHHHCCCEEEEECCCCCHHHCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHEE
TVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLPTVAPAFENTIKTFFPIPKHI
EHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCHHHH
FEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCCCEEEEEEEECCCC
AANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQA
CEEEEEECCCEEEEECCCCCCCHHHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHHHH
LSYALDREEIIDAAYGSDEYAKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDT
HHHHCCHHHHHHHHCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDPNALSNITLDRKNADYSIALN
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHCEEEECCCCCEEEEEE
GYIMGNDPDAYKSLYLSDAPYNYSNYHNKDLDALWEKGAVTADDKERQEIYEKIQNTIAD
EEEECCCCHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
DAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE
CEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKFLLVAVISVFALVLTACGGSGASSDKANGSGKAKDGGSLIIGVTGDPEVINPNYASD
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEECCCCCCC
RVTLTIQQAVYAPLFWEVDGKPALAKSLDISDDNLTYTVKLKDGLTWHDGKPLTADDVVF
EEEEEEHHHHCCCEEEEECCCCCHHHCCCCCCCCEEEEEEECCCCEECCCCCCCCCHHEE
TVNSILDTKQNSPNRGNFVFDDKPVKVEAVDDTTVKFTLPTVAPAFENTIKTFFPIPKHI
EHHHHHHCCCCCCCCCCEEECCCCEEEEEECCCEEEEECCCCCHHHHHHHHHHCCCHHHH
FEGVENIEKSDKNKNPIGSGPYKFVEYKTGEYVSLERFNDYFDGKPKLDKVTFRITKDQN
HHHHHHHHHCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHCCCCCCCEEEEEEEECCCC
AANLALQNGEINLKSIQPSDRNKVEKASAVNIITYPENRLSYATFNENQPALKSKELRQA
CEEEEEECCCEEEEECCCCCCCHHHHHHEEEEEECCCCCEEEEEECCCCCCHHHHHHHHH
LSYALDREEIIDAAYGSDEYAKPASSFLTENTKYFTDKVETYDQDIAKAKKLVKESGFDT
HHHHCCHHHHHHHHCCCCHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SQKLTVYYLNNSKSQESIALYLQQQYKEIGVTLDLKPTDPNALSNITLDRKNADYSIALN
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCHHCEEEECCCCCEEEEEE
GYIMGNDPDAYKSLYLSDAPYNYSNYHNKDLDALWEKGAVTADDKERQEIYEKIQNTIAD
EEEECCCCHHHHEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHCC
DAVIYPISYDNAVLALDSRYGGQKAATPQPVTMFRDLSKLYLTE
CEEEEEEECCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]