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The map label for this gene is purR [H]

Identifier: 226222676

GI number: 226222676

Start: 39577

End: 40632

Strand: Reverse

Name: purR [H]

Synonym: Lm4b_00040

Alternate gene names: 226222676

Gene position: 40632-39577 (Counterclockwise)

Preceding gene: 226222718

Following gene: 226222675

Centisome position: 1.39

GC content: 37.41

Gene sequence:

>1056_bases
ATGAAAAAAATAACCATACAAGAAATAGCTAAACTTAGCGGTGTCTCCGTTTCAACTGTTTCGCGGGTTCTTAATAACAG
TCCTTCTGTGTCAGCTGCGAAACGCAAAAAAATCCAAGCAATCATTGATGAACATAATTACACACCTAGCGTGTTTGCTC
GTGGGATGGTCAATAAGCAGACGAAGAATATAGGCGTTATTTTACCAGATATTTCCAATCCCTATTTCATTTCACTTATA
ACGCAAATTCAAAAATTCGCGCTAGATTATATGTTTTCGACGATTTTGTTCAATACAATGCTTGCGGAGCCAAATAATAA
GAATAATCAACATCCCCTAACGGAAGAAGATTACCTAAAAATCATTTTAGAAAAGCAAGTGGATGGTTTACTTATTTTAG
GTGGAGAAATCGATAAAGAAATCGTTCCAAAAGAGTATATAACTGCTCTAAATCAGTTAAATAATGAAATTCCAGTTGTT
GTTATCGGGTCGAAAATTCCTGAGCTCAACTGTTTATTTATTGAACGTAATTTAAAAAAAGGGGTAACAACCTTAGTTAG
TCATCTAACTGCACTTGGACACAAAAATATTGGTTTTATTGGCGGAGAAGCTGGTGTAAAAATCACCACCTACCGTCTAG
AATCTTTTAAAGAAGCAATGACTAGCTATCAACATCCGGTAAATGACGACTGGGTGGTTCTGTCTGATTATTATACGGCT
GATGGCTACGCAGCAATGACTCAACTTTTAGAAAATAGCGCTACTTTGCCGACAGCACTTGTAGCTATCAATGACAATGT
GGCTATCGGGGCAATTCGAGCGATTAATGATGCCAAACTTTCTTGCCCTGAAGACATCGCTATTGTCAGCTGCGATCAAT
TTATGAATGGCGATTACCAAACTCCGCGTTTAACTTCCATGGATCAACACAATGAATATCTTGGTAAAATGGCTATTTTA
CAACTAATTAGCGCAATTAACGGTCAAGTCGAGCCAATGATTATCAACCATAATCCAGAACTAATTATCCGCGAATCTTG
CGGCTCTAAGCTATAA

Upstream 100 bases:

>100_bases
TATTATATTTTTGATAACGTTACCAAATTTCACTTGTTATTTCCCCCTAAGTACGCTACACTTAAAATAGTTAAAAATGC
TTACGAGGTGAACATTTCGA

Downstream 100 bases:

>100_bases
CGAAAGGAAGGTCCACTTGGAAAAATACTTAATTTGTTCTGATTTAGACGGGACATTACTACTTAAAAACCAAACTATCT
CACCAAAAACGTTAAATTTA

Product: transcription regulator LacI

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKITIQEIAKLSGVSVSTVSRVLNNSPSVSAAKRKKIQAIIDEHNYTPSVFARGMVNKQTKNIGVILPDISNPYFISLI
TQIQKFALDYMFSTILFNTMLAEPNNKNNQHPLTEEDYLKIILEKQVDGLLILGGEIDKEIVPKEYITALNQLNNEIPVV
VIGSKIPELNCLFIERNLKKGVTTLVSHLTALGHKNIGFIGGEAGVKITTYRLESFKEAMTSYQHPVNDDWVVLSDYYTA
DGYAAMTQLLENSATLPTALVAINDNVAIGAIRAINDAKLSCPEDIAIVSCDQFMNGDYQTPRLTSMDQHNEYLGKMAIL
QLISAINGQVEPMIINHNPELIIRESCGSKL

Sequences:

>Translated_351_residues
MKKITIQEIAKLSGVSVSTVSRVLNNSPSVSAAKRKKIQAIIDEHNYTPSVFARGMVNKQTKNIGVILPDISNPYFISLI
TQIQKFALDYMFSTILFNTMLAEPNNKNNQHPLTEEDYLKIILEKQVDGLLILGGEIDKEIVPKEYITALNQLNNEIPVV
VIGSKIPELNCLFIERNLKKGVTTLVSHLTALGHKNIGFIGGEAGVKITTYRLESFKEAMTSYQHPVNDDWVVLSDYYTA
DGYAAMTQLLENSATLPTALVAINDNVAIGAIRAINDAKLSCPEDIAIVSCDQFMNGDYQTPRLTSMDQHNEYLGKMAIL
QLISAINGQVEPMIINHNPELIIRESCGSKL
>Mature_351_residues
MKKITIQEIAKLSGVSVSTVSRVLNNSPSVSAAKRKKIQAIIDEHNYTPSVFARGMVNKQTKNIGVILPDISNPYFISLI
TQIQKFALDYMFSTILFNTMLAEPNNKNNQHPLTEEDYLKIILEKQVDGLLILGGEIDKEIVPKEYITALNQLNNEIPVV
VIGSKIPELNCLFIERNLKKGVTTLVSHLTALGHKNIGFIGGEAGVKITTYRLESFKEAMTSYQHPVNDDWVVLSDYYTA
DGYAAMTQLLENSATLPTALVAINDNVAIGAIRAINDAKLSCPEDIAIVSCDQFMNGDYQTPRLTSMDQHNEYLGKMAIL
QLISAINGQVEPMIINHNPELIIRESCGSKL

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=345, Percent_Identity=31.5942028985507, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1790194, Length=323, Percent_Identity=31.2693498452012, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790369, Length=325, Percent_Identity=27.6923076923077, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789202, Length=304, Percent_Identity=26.3157894736842, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1788474, Length=352, Percent_Identity=25.5681818181818, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=26.865671641791, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789068, Length=338, Percent_Identity=25.1479289940828, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787580, Length=338, Percent_Identity=26.6272189349112, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI48994940, Length=340, Percent_Identity=26.7647058823529, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790689, Length=339, Percent_Identity=24.7787610619469, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787906, Length=275, Percent_Identity=26.5454545454545, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789456, Length=350, Percent_Identity=24.5714285714286, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786268, Length=315, Percent_Identity=21.2698412698413, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790715, Length=329, Percent_Identity=22.7963525835866, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 38838; Mature: 38838

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITIQEIAKLSGVSVSTVSRVLNNSPSVSAAKRKKIQAIIDEHNYTPSVFARGMVNKQ
CCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
TKNIGVILPDISNPYFISLITQIQKFALDYMFSTILFNTMLAEPNNKNNQHPLTEEDYLK
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IILEKQVDGLLILGGEIDKEIVPKEYITALNQLNNEIPVVVIGSKIPELNCLFIERNLKK
HHHHHCCCCEEEECCCCCCHHCCHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECHHHH
GVTTLVSHLTALGHKNIGFIGGEAGVKITTYRLESFKEAMTSYQHPVNDDWVVLSDYYTA
HHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DGYAAMTQLLENSATLPTALVAINDNVAIGAIRAINDAKLSCPEDIAIVSCDQFMNGDYQ
CHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCCC
TPRLTSMDQHNEYLGKMAILQLISAINGQVEPMIINHNPELIIRESCGSKL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCC
>Mature Secondary Structure
MKKITIQEIAKLSGVSVSTVSRVLNNSPSVSAAKRKKIQAIIDEHNYTPSVFARGMVNKQ
CCCCCHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCC
TKNIGVILPDISNPYFISLITQIQKFALDYMFSTILFNTMLAEPNNKNNQHPLTEEDYLK
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
IILEKQVDGLLILGGEIDKEIVPKEYITALNQLNNEIPVVVIGSKIPELNCLFIERNLKK
HHHHHCCCCEEEECCCCCCHHCCHHHHHHHHHCCCCCCEEEECCCCCCCEEEEEECHHHH
GVTTLVSHLTALGHKNIGFIGGEAGVKITTYRLESFKEAMTSYQHPVNDDWVVLSDYYTA
HHHHHHHHHHHHCCCCCEEECCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
DGYAAMTQLLENSATLPTALVAINDNVAIGAIRAINDAKLSCPEDIAIVSCDQFMNGDYQ
CHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEEECCCCCCCCCCEEEEEEHHHCCCCCC
TPRLTSMDQHNEYLGKMAILQLISAINGQVEPMIINHNPELIIRESCGSKL
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]