Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 225861586

GI number: 225861586

Start: 1622357

End: 1623100

Strand: Reverse

Name: rsmE [H]

Synonym: SPT_1704

Alternate gene names: 225861586

Gene position: 1623100-1622357 (Counterclockwise)

Preceding gene: 225861587

Following gene: 225861585

Centisome position: 76.85

GC content: 45.03

Gene sequence:

>744_bases
ATGCAACAGTATTTTGTCAAGGGGAGTGCAGTCTCTCCTGTCACCATCGAGGACAGGGAAACCAGCAAGCATATGTTTCA
GGTTATGCGCTTGAAAGAAGATGATGAGGTTACTTTGGTCTTTGATGATGGAATTAAGCGCTTGGCGCGTGTGCTGGATG
TGGAAGCTCGTCAGTTTGAATTGGTAGAAGAACTAGATGACAATGTGGAACTGCCCGTTCAAGTGACCATTTCATCTGGT
TTTCCCAAGGGAGATAAGTTGGAGTTTATCACTCAAAAGGTGACGGAACTAGGTGCCAGCCAGATTTGGGCCTTCCCTGC
CGATTGGTCCGTTGCTAAGTGGGATGGCAAGAAATTGGGTAAAAAGGTTGAAAAACTAGAAAAAATTGCCCTTGGAGCAG
CCGAGCAAAGCAAGCGTAATATTGTACCAAGTATTAAACTTTTCGAGAAAAAAGCAGATTTTCTAGCTCAGCTGGACCAG
TTTGACTCTATCATAGTAGCCTATGAAGAATCAGCTAAAGAAGGAGAAGCCGCTGCGCTTCTGCAAGCAGTCACTGGTCT
TGAAACAGGAGCCAAACTGCTCTTTATCTTTGGTCCAGAAGGCGGTCTCTCACCTGCAGAAATCGAAAGTTTTGAAGCTA
AAGGAGCCGTTTTGGCAGGCCTAGGTCCTCGTATTTTGCGAGCAGAAACAGCACCGCTTTACGCTTTATCAGCCCTTAGT
GTTTTAGTAGAATTAGAGAAATAA

Upstream 100 bases:

>100_bases
TCAGCTGGATTTTTCCTCGAAACTCACATGGTTCAAGGGGAATGGAATACCTGTGTCTTTAAGAAAACCAAGGATATCTC
TGGTGTGATTGGAGGCTAGG

Downstream 100 bases:

>100_bases
GAGGAAGAAAATGGAACAAAAACACCGTTCAGAATTTCCAGAGAAGGAACTCTGGGACTTAACAGCCCTATACCAAGACC
GTGAGGATTTCTTGCGTGCA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDGIKRLARVLDVEARQFELVEELDDNVELPVQVTISSG
FPKGDKLEFITQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK

Sequences:

>Translated_247_residues
MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDGIKRLARVLDVEARQFELVEELDDNVELPVQVTISSG
FPKGDKLEFITQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK
>Mature_247_residues
MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDGIKRLARVLDVEARQFELVEELDDNVELPVQVTISSG
FPKGDKLEFITQKVTELGASQIWAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
VLVELEK

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=229, Percent_Identity=28.3842794759825, Blast_Score=82, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27128; Mature: 27128

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDGIKRLARVLDVEARQFE
CCCEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCHHHHHH
LVEELDDNVELPVQVTISSGFPKGDKLEFITQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHEECCCCCHHHHHHHH
VLVELEK
HHHEECC
>Mature Secondary Structure
MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEDDEVTLVFDDGIKRLARVLDVEARQFE
CCCEEECCCCCCCEEECCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCHHHHHH
LVEELDDNVELPVQVTISSGFPKGDKLEFITQKVTELGASQIWAFPADWSVAKWDGKKLG
HHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCHHEEECCCCCCEEECCHHHHH
KKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQFDSIIVAYEESAKEGEAAAL
HHHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHH
LQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVLAGLGPRILRAETAPLYALSALS
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCHHEECCCCCHHHHHHHH
VLVELEK
HHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]