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The map label for this gene is tkt1 [H]

Identifier: 225861446

GI number: 225861446

Start: 1476362

End: 1478332

Strand: Reverse

Name: tkt1 [H]

Synonym: SPT_1555

Alternate gene names: 225861446

Gene position: 1478332-1476362 (Counterclockwise)

Preceding gene: 225861447

Following gene: 225861445

Centisome position: 69.99

GC content: 44.55

Gene sequence:

>1971_bases
ATGTCACTACAATCAGTTAACGCCATTCGTTTTCTTGGCGTAGATGCTATTAACAAATCTAATTCTGGTCACCCGGGAAT
CGTCATGGGTGCTGCGCCAATGGCTTATAGCCTATTTACAAAGCACCTTAGAATTACACCTGAGCAGCCAAACTGGATTA
ACCGAGATCGCTTTATCTTGTCTGCGGGTCATGGATCAATGCTACTGTATGCTCTCTTGCATTTAACAGGGTATAACGAT
GTATCCATGGACGAGATTAAAAATTTCCGGCAATGGGGATCTAAGACACCTGGTCATCCTGAAGTGACGCATACGTCTGG
TGTGGATGCGACATCTGGTCCGCTTGGTCAGGGAATTTCTACTGCCGTTGGTTTCGCCCAAGCAGAGCGTTTTTTAGCTG
CTAAGTACAACAAAGATGGTTTCCCTATTTTTGACCATTATACTTATGTTATTGCTGGAGACGGTGACTTCATGGAAGGA
GTGTCTGCGGAGGCGGCTTCTTATGCAGGTCATCAAGCTTTAGATAAGCTTATCGTCCTCTACGACTCCAACGACATCTG
CTTGGATGGTGAGACCAAAGATACTTTCTCTGAAAATGTTCGCGCCCGTTACGATGCTTATGGTTGGCATACAGTTCTGG
TAGAAGATGGAACAGATTTAGCAGCAATTTCTACAGCAATTGAGACGGCCAAGTTTTCTGGTAAGCCGAGTTTGATTGAA
GTGAAAACGGTAATTGGTTACGGCTCACCCAATAAAAGTGGTACAAATGCTGTTCATGGTGCACCACTAGGAGCAGAAGA
AACAGGAGCAACTCGTAAGTTTTTGGGATGGGATTACGATCCATTTGAAGTACCAGAGGAAGTATATTCTGATTTCAAGA
CAAATGTAGCGGATCGTGGTCAGGAGGCATACGATGCTTGGGCTAGTTTGGTGTCTGATTACAAGGTTGCTTATCCCGAA
GTTGCTAGTGAGATTGACGCTATTGTAGCTGGAAAATTCCCTGTAACCATTACTGAAAAAGACTTCCCTGTCTATGAGAA
TGGCTTCTCTCAAGCAACTCGTAATTCGTCCCAAGATGCTATTAATACAGCAGCAGCAGTTTTACCAACCTTCTTAGGTG
GATCGGCAGACTTAGCTCACTCCAACATGACCTACATCAAGGCAGATGGCTTACAAGATAAATATAATCCATTAAACCGC
AATATTCAGTTTGGGGTACGTGAATTTGCCATGGGAACAATCCTCAATGGAATGGCTCTTCATGGTGGTTTACGAGTTTA
TGGCGGAACCTTCTTTGTTTTCTCTGACTACGTCAAAGCTGCTATTCGGCTATCAGCCATTCAGGAGTTGCCTGTAACTT
ATGTCTTTACCCATGATTCAATTGCCGTTGGTGAAGATGGTCCAACTCATGAACCAGTTGAACATTTGGCAGGTTTACGC
TCAATGCCAAACTTGACTGTTATCCGTCCAGCGGATGCCCGTGAAACTCAAGCGGCTTGGCATCATGCCTTGACCAGTAC
CACCACTCCAACTGTCATTGTCTTAACCCGTCAAAACTTGGTAGTTGAAGAAGGGACAGACTTTGGTAAGGTCGCTAAAG
GAGCCTACGTCGTGTATGATACCCCGGGATTTGATACTATTATCATTGCTACAGGATCTGAGGTCAATCTAGCTATCAAA
GCTGCTAAGGAATTGGTTTTACAAGGTGGTAAAGTACGTGTGGTATCTATGCCCTCAACCGAACTATTTGATGCTCAAGA
TGCTACCTACAAGGAAGACATTTTACCATCTAAGACTCGTCGTCGTGTGGCCATTGAAATGGCAGCGACCCAAAGTTGGT
ACAAGTATGTTGGTTTGGATGGCGCGGTCATCGGTATTGACATCTTCGGTGCGTCTGCCCCAGCTCAGACTGTGATTGAT
AATTATGGATTTACGGTAGAGAATATCGTTGCTCAAGTTAAGTCCCTATAG

Upstream 100 bases:

>100_bases
AGCCTGGGATATCTGTTCTAGAGATTACGAAGAAATGACCGGAAAAACAATGCCAATCAAATAATGGTTTGAGAAGAAAT
TTATTAGTTAGGAGGAATAT

Downstream 100 bases:

>100_bases
AAACCAATTACAATGAAGATACAGCTGTTGTCAGACTAGCAGATGTAGTGATAGACACTAATCAGATGATTGGTTATTTA
AAAACTATAATGAGAATGTA

Product: transketolase

Products: NA

Alternate protein names: TK [H]

Number of amino acids: Translated: 656; Mature: 655

Protein sequence:

>656_residues
MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYND
VSMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIE
VKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPE
VASEIDAIVAGKFPVTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNR
NIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
SMPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIK
AAKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVID
NYGFTVENIVAQVKSL

Sequences:

>Translated_656_residues
MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYND
VSMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEG
VSAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIE
VKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPE
VASEIDAIVAGKFPVTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNR
NIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
SMPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIK
AAKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVID
NYGFTVENIVAQVKSL
>Mature_655_residues
SLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFILSAGHGSMLLYALLHLTGYNDV
SMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGISTAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEGV
SAEAASYAGHQALDKLIVLYDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIEV
KTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRGQEAYDAWASLVSDYKVAYPEV
ASEIDAIVAGKFPVTITEKDFPVYENGFSQATRNSSQDAINTAAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNRN
IQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLRS
MPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYDTPGFDTIIIATGSEVNLAIKA
AKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTRRRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVIDN
YGFTVENIVAQVKSL

Specific function: Necessary for high-efficiency recombination chromosomal DNA during genetic transformation (PubMed:2361942) [H]

COG id: COG0021

COG function: function code G; Transketolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transketolase family [H]

Homologues:

Organism=Homo sapiens, GI205277463, Length=581, Percent_Identity=25.9896729776248, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI4507521, Length=581, Percent_Identity=25.9896729776248, Blast_Score=154, Evalue=3e-37,
Organism=Homo sapiens, GI133778974, Length=590, Percent_Identity=27.1186440677966, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI225637459, Length=596, Percent_Identity=22.3154362416107, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225637463, Length=522, Percent_Identity=23.3716475095785, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI225637461, Length=522, Percent_Identity=23.3716475095785, Blast_Score=103, Evalue=5e-22,
Organism=Escherichia coli, GI48994911, Length=658, Percent_Identity=48.0243161094225, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1788808, Length=659, Percent_Identity=47.4962063732929, Blast_Score=600, Evalue=1e-173,
Organism=Caenorhabditis elegans, GI17539652, Length=677, Percent_Identity=25.1107828655835, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6325331, Length=660, Percent_Identity=46.2121212121212, Blast_Score=551, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6319593, Length=665, Percent_Identity=44.3609022556391, Blast_Score=532, Evalue=1e-152,
Organism=Drosophila melanogaster, GI45551847, Length=682, Percent_Identity=25.6598240469208, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI45550715, Length=682, Percent_Identity=25.6598240469208, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24666278, Length=580, Percent_Identity=28.448275862069, Blast_Score=158, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24645119, Length=653, Percent_Identity=25.5742725880551, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005478
- InterPro:   IPR020826
- InterPro:   IPR005476
- InterPro:   IPR005474 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C; PF00456 Transketolase_N [H]

EC number: =2.2.1.1 [H]

Molecular weight: Translated: 70918; Mature: 70787

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00801 TRANSKETOLASE_1 ; PS00802 TRANSKETOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFIL
CCCCCCCEEEEEECEECCCCCCCCCCEEEECCHHHHHHHHHHEEECCCCCCCCCCCEEEE
SAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGIS
ECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHH
TAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEGVSAEAASYAGHQALDKLIVL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECCCHHHHCCCHHHHHHHHHHHHCEEEEE
YDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIE
ECCCCEEECCCCCHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
VKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRG
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHCCCHHHHHHHHHCHHHCC
QEAYDAWASLVSDYKVAYPEVASEIDAIVAGKFPVTITEKDFPVYENGFSQATRNSSQDA
HHHHHHHHHHHHCCHHCCHHHHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHCCCCHHH
INTAAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNRNIQFGVREFAMGTILNGMAL
HHHHHHHHHHHCCCCCHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
HGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
CCCEEEECCEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCCCHHHHHHHHH
SMPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHCCEEEEEE
TPGFDTIIIATGSEVNLAIKAAKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTR
CCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHCCCCCHH
RRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVIDNYGFTVENIVAQVKSL
CEEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure 
SLQSVNAIRFLGVDAINKSNSGHPGIVMGAAPMAYSLFTKHLRITPEQPNWINRDRFIL
CCCCCCEEEEEECEECCCCCCCCCCEEEECCHHHHHHHHHHEEECCCCCCCCCCCEEEE
SAGHGSMLLYALLHLTGYNDVSMDEIKNFRQWGSKTPGHPEVTHTSGVDATSGPLGQGIS
ECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCCHH
TAVGFAQAERFLAAKYNKDGFPIFDHYTYVIAGDGDFMEGVSAEAASYAGHQALDKLIVL
HHHHHHHHHHHHHHHCCCCCCEEEECEEEEEECCCHHHHCCCHHHHHHHHHHHHCEEEEE
YDSNDICLDGETKDTFSENVRARYDAYGWHTVLVEDGTDLAAISTAIETAKFSGKPSLIE
ECCCCEEECCCCCHHHHHHHHEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEE
VKTVIGYGSPNKSGTNAVHGAPLGAEETGATRKFLGWDYDPFEVPEEVYSDFKTNVADRG
EEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCHHCCCHHHHHHHHHCHHHCC
QEAYDAWASLVSDYKVAYPEVASEIDAIVAGKFPVTITEKDFPVYENGFSQATRNSSQDA
HHHHHHHHHHHHCCHHCCHHHHHHHHHEEECCCEEEEECCCCCCCCCCHHHHHCCCCHHH
INTAAAVLPTFLGGSADLAHSNMTYIKADGLQDKYNPLNRNIQFGVREFAMGTILNGMAL
HHHHHHHHHHHCCCCCHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHH
HGGLRVYGGTFFVFSDYVKAAIRLSAIQELPVTYVFTHDSIAVGEDGPTHEPVEHLAGLR
CCCEEEECCEEEEHHHHHHHHHHHHHHHHCCEEEEEECCCEEECCCCCCCCHHHHHHHHH
SMPNLTVIRPADARETQAAWHHALTSTTTPTVIVLTRQNLVVEEGTDFGKVAKGAYVVYD
CCCCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCEEEECCCCHHHHHCCEEEEEE
TPGFDTIIIATGSEVNLAIKAAKELVLQGGKVRVVSMPSTELFDAQDATYKEDILPSKTR
CCCCCEEEEEECCCCEEHHHHHHHHHHCCCCEEEEECCCCHHCCCCCCCHHHHCCCCCHH
RRVAIEMAATQSWYKYVGLDGAVIGIDIFGASAPAQTVIDNYGFTVENIVAQVKSL
CEEEEEEHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2361942; 11463916; 8248627 [H]