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The map label for this gene is insK [H]

Identifier: 225861445

GI number: 225861445

Start: 1474718

End: 1475989

Strand: Reverse

Name: insK [H]

Synonym: SPT_1554

Alternate gene names: 225861445

Gene position: 1475989-1474718 (Counterclockwise)

Preceding gene: 225861446

Following gene: 225861442

Centisome position: 69.88

GC content: 36.24

Gene sequence:

>1272_bases
ATGAAATTGAGTTATGAAGATAAAGTTCAGATCTATGAACTAAGAAAGCAAGGACAAAGCTTCAAACAGCTTTCAAAAAG
ATTTGGTGTGGATGTTTCTGGTCTAAAGTACATGGTGAAATTAATTGACCGTTATGGCATAGAAATCGTTAAAAAGGGGA
AGAATCGTCACTATTCTTCTAAATTGAAACAAGAAATGATGGATAAAGTCTTACTGGAAGGTTGTTCACAAAGAAGTGTT
AGTCTTGATTATGCCCTCCCAAACCAAGGAATGCTTTCATTTTGGCTGGCACAATACAAGAAAAACGGGTATACTATTGT
TGAGAAAACAAGAGAGAGAGTACCTGAGAGCGGAGAATGCCATCCTAAAAAAGTTAAGAGAACTCCGATTGAAGGAGGAA
AAAGAGAAAGAAGAAAGACAGAAATTGTTCAAGAATTAATGACTGAGTTTTCGTTAGTTCTTCTTCTAAAATCCATTAAA
CTAGCTCGTTGGACCTACTACTATCACTTGAAACAGCTAGACAAAACAGATAAGGACCAAGAGCTTAAAGCTGAAATTCA
ATCCATTTTTATCGAACACAAAGGAAATTATGCTTATCGCCGGGTTCATTTAGAACTAAGAAATCGTGCTTATCTGGTAA
ATCATAAAAGAGTTCAAGGCTTGATAAAAGTACTCAATTTACAAGCTAAAATGCGACAGAAACGAAAATATTCTTCTCAT
AAAGGAGACGTTGGCAAGAAGGCAGAGAATCTCATTCAAGGCCAATTTGAAGGCTCTAAAACAATGGAAAAGTGCTACAC
AGATGTGACAGAATTTGCCATTCCAGCAAGTACTCAAAAGCTTTACTTATCACCAGTTTTAGATGGCTTTAACAGCGAAA
TTATTGCTTTTAATCTTTCAACTTCGCCCAACTTAGAACAAGTGAAGAGTATGTTAGAACAGGCCTTTACAGAGAAATAC
TATGAGAATACGATTCTCCATAGTGACCAAGGCTGGCAATACCGACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGG
AGTTCAAGCATCCATGTCACGTAAGGGTAACAGCCAAGACAACGGTATGATGGAGTCCTTCTTTGGGATTCTGAAATCGG
AAATGTTTTATGGCTATGAGAAAACATTTAAATCACTTAATCAATTGGAACAAGCTATTGTAGACTATATTGATTACTAC
AACAATAAACGAATTAAGGTAAAACTAAAAGGACTTAGCCCTGTGCAATACAGAACTAAATCCTTTGGATAA

Upstream 100 bases:

>100_bases
AGGTCTCTAAAATTAAAAAACGCATAGTATAGGATGTTGAAATGATGAACTGCACCCCAAAAGTTAGACAGAATAAATCT
AACTTTTGGGGTGTTTTATT

Downstream 100 bases:

>100_bases
ATTATTTGTCTAACTGTTTGAGGGGCAGTACATTTTTTTGGTGTATATAAAATTTGTAGGAGCTATATCTACAATTTTAT
ATCCCAGTTTATGGATGTCA

Product: IS3-Spn1, transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKVLLEGCSQRSV
SLDYALPNQGMLSFWLAQYKKNGYTIVEKTRERVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIK
LARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSH
KGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSTSPNLEQVKSMLEQAFTEKY
YENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYY
NNKRIKVKLKGLSPVQYRTKSFG

Sequences:

>Translated_423_residues
MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKVLLEGCSQRSV
SLDYALPNQGMLSFWLAQYKKNGYTIVEKTRERVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIK
LARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSH
KGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSTSPNLEQVKSMLEQAFTEKY
YENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYY
NNKRIKVKLKGLSPVQYRTKSFG
>Mature_423_residues
MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKVLLEGCSQRSV
SLDYALPNQGMLSFWLAQYKKNGYTIVEKTRERVPESGECHPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIK
LARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSH
KGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLSTSPNLEQVKSMLEQAFTEKY
YENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYY
NNKRIKVKLKGLSPVQYRTKSFG

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=298, Percent_Identity=27.5167785234899, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1789981, Length=282, Percent_Identity=46.0992907801418, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1788405, Length=288, Percent_Identity=27.4305555555556, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787263, Length=288, Percent_Identity=27.4305555555556, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786753, Length=288, Percent_Identity=27.4305555555556, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786570, Length=288, Percent_Identity=27.4305555555556, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786490, Length=288, Percent_Identity=27.4305555555556, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 49611; Mature: 49611

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIVKKGKNRHYSS
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHCCCCCCHHH
KLKQEMMDKVLLEGCSQRSVSLDYALPNQGMLSFWLAQYKKNGYTIVEKTRERVPESGEC
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
HPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQ
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSH
HHHHHHHHHHHHCCCCEEEEEEEHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
KGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCCCCEEEEECC
TSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQD
CCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
NGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHC
SFG
CCC
>Mature Secondary Structure
MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDRYGIEIVKKGKNRHYSS
CCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHCCCCCCHHH
KLKQEMMDKVLLEGCSQRSVSLDYALPNQGMLSFWLAQYKKNGYTIVEKTRERVPESGEC
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCC
HPKKVKRTPIEGGKRERRKTEIVQELMTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQ
CCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
ELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSH
HHHHHHHHHHHHCCCCEEEEEEEHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCC
KGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAFNLS
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHEEHHHHHCCCCCCEEEEECC
TSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYRHDSYHRFLESKGVQASMSRKGNSQD
CCCCHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
NGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHC
SFG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]