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The map label for this gene is truA [H]

Identifier: 225861429

GI number: 225861429

Start: 1463212

End: 1463961

Strand: Reverse

Name: truA [H]

Synonym: SPT_1537

Alternate gene names: 225861429

Gene position: 1463961-1463212 (Counterclockwise)

Preceding gene: 225861431

Following gene: 225861428

Centisome position: 69.31

GC content: 44.4

Gene sequence:

>750_bases
ATGACGAGATATAAAGCAACTATTTCCTATGATGGTTATGCCTTTGCTGGCTTTCAGCGCCAGCCTCATGCGCGTAGCGT
TCAGGAAGAAATTGAAAAAACCTTGACCAGATTAAATAAAGGGCAAACCATTACTGTTCACGGTGCTGGTAGGACAGATA
GTGGGGTTCATGCCCTGGGACAGGTCATTCATTTTGACCTGCCTTATCAGATGGATGAGGAGAAACTCCGTTTTGCCTTG
GATACCCAGTCTCCTGAAGATATTGATGTGATTTCGATTGAGCTTGTGGCAGCTGATTTTCATTGCCGTTATGCCAAGCA
TAGCAAGACCTATGAGTTTATTGTGGATAGAGGACGTCCCAAAAATCCGATGCGCCGTCACTATGCCACCCACTTTCCCT
ACCCACTCGATGTGGAACGAATGCAGATTGCAATCAAAAAGCTAGAGGGAACCCATGATTTTACCGGTTTTACAGCCTCT
GGGACTAGTGTAGAGGATAAGGTTCGCACCATCACAGAAGCTAGTTTAATAGTCGATGAGACAGGACAATTTTTGACCTT
TACCTTTTCCGGAAATGGTTTCTTGTATAAACAGATTCGCAATATGGTGGGGACTCTTCTCAAAATCGGTAACAACCGCA
TGCCAGTAGAGCAGATTGATCTCATCTTGGAGAAGAAGGACAGGCAACTTGCAGGTCCCACTGCAGCACCAAATGGTTTG
TATTTAAAGGAGATTCGTTATGAAGAATAA

Upstream 100 bases:

>100_bases
GTGGTTTGAAGAGATTTTCAAGAGTATAAGATTTTTATACCCATACAATATTTGTTTTAGGTCCTTGTTTACAGACTAAT
TTCTTGATAAAATAGAGGTT

Downstream 100 bases:

>100_bases
TCGTATTTTAGCACTTTCTGGAAATGATATTTTTAGTGGTGGTGGACTGTCAGCTGATTTGGCTACCTATACCTTGAACG
GCTTGCATGGGTTTGTAGCA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVAADFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE

Sequences:

>Translated_249_residues
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFAL
DTQSPEDIDVISIELVAADFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
YLKEIRYEE
>Mature_248_residues
TRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALD
TQSPEDIDVISIELVAADFHCRYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASG
TSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGLY
LKEIRYEE

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=276, Percent_Identity=31.8840579710145, Blast_Score=102, Evalue=2e-22,
Organism=Homo sapiens, GI199559785, Length=278, Percent_Identity=27.6978417266187, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1788657, Length=242, Percent_Identity=35.9504132231405, Blast_Score=159, Evalue=2e-40,
Organism=Drosophila melanogaster, GI116007752, Length=312, Percent_Identity=27.5641025641026, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281362146, Length=259, Percent_Identity=25.0965250965251, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648455, Length=259, Percent_Identity=25.0965250965251, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28379; Mature: 28247

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALG
CCEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHCRYAKHSKTYEFIVDRGRP
HHHEECCCCCCCHHHEEEEECCCCCCCCEEEEEEEEEECCCEEHHCCCCCEEEEEECCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC
>Mature Secondary Structure 
TRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTITVHGAGRTDSGVHALG
CEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHH
QVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVAADFHCRYAKHSKTYEFIVDRGRP
HHHEECCCCCCCHHHEEEEECCCCCCCCEEEEEEEEEECCCEEHHCCCCCEEEEEECCCC
KNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTASGTSVEDKVRTITEASLIVDE
CCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHHHHHHHEEEEC
TGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQIDLILEKKDRQLAGPTAAPNGL
CCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCE
YLKEIRYEE
EEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA