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The map label for this gene is folA [H]

Identifier: 225861404

GI number: 225861404

Start: 1441267

End: 1441773

Strand: Reverse

Name: folA [H]

Synonym: SPT_1511

Alternate gene names: 225861404

Gene position: 1441773-1441267 (Counterclockwise)

Preceding gene: 225861405

Following gene: 225861403

Centisome position: 68.26

GC content: 41.42

Gene sequence:

>507_bases
ATGACTAAGAAAATCGTAGCTATTTGGGCCCAGGATGAAGAGGGTGTGATTGGTAAGGACAATCGTCTGCCTTGGCATTT
GCCGGCAGAATTGCAACACTTTAAAGAAACAACTCTGAATCATGCTATCTTGATGGGGCGTGTGACCTTTGATGGAATGG
GGCGTCGCTTGCTTCCCAAACGGGAGACCTTGATTTTGACTCGTAACCCAGAAGAAAAGATAGACGGGGTTGCTACTTTT
TATGATGTTCAGTCTGTCTTGGACTGGTATCAGGCTCAAGATAAGAATCTTTATATTTTAGGTGGAAAGCAAATTTTTCA
AGCTTTTGAACCCTACCTTGACGAAGTGATTGTGACTCACATTCATGCTCGGGTGGAAGGAGATACCTATTTCCCTGAAG
AATTTGACTTGTCTCTTTTTGAGACAGTTTCAAGCAAATTCTATAGCAAAGATGAGAAAAATCCTTATGATTTTACCATC
CAATACCGCAAGAGAAAGGAAGTCTAA

Upstream 100 bases:

>100_bases
GAAAATTCTTACAGATTTTTTCTATTTTGTAAGCTATTCCAAACCAGTCTTTTTTTGAGTTTTTTGATAAAATAGTACTA
TCAGTAAAAAGGATGGAAGC

Downstream 100 bases:

>100_bases
TGGAACGTAGTATATTTGGTTTTTTCACAGCCATGCTGTGTTTGGTCTGCTTGCTTGCAGGGGCACAGGCTTTTCGTAAA
AAGCGCTATGGACTTTCTGT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 168; Mature: 167

Protein sequence:

>168_residues
MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATF
YDVQSVLDWYQAQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYSKDEKNPYDFTI
QYRKRKEV

Sequences:

>Translated_168_residues
MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATF
YDVQSVLDWYQAQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYSKDEKNPYDFTI
QYRKRKEV
>Mature_167_residues
TKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFY
DVQSVLDWYQAQDKNLYILGGKQIFQAFEPYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYSKDEKNPYDFTIQ
YRKRKEV

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=153, Percent_Identity=35.9477124183007, Blast_Score=98, Evalue=2e-22,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 19761; Mature: 19630

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPK
CCCEEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCC
RETLILTRNPEEKIDGVATFYDVQSVLDWYQAQDKNLYILGGKQIFQAFEPYLDEVIVTH
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHH
IHARVEGDTYFPEEFDLSLFETVSSKFYSKDEKNPYDFTIQYRKRKEV
HHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure 
TKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAILMGRVTFDGMGRRLLPK
CCEEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCC
RETLILTRNPEEKIDGVATFYDVQSVLDWYQAQDKNLYILGGKQIFQAFEPYLDEVIVTH
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHHHH
IHARVEGDTYFPEEFDLSLFETVSSKFYSKDEKNPYDFTIQYRKRKEV
HHHEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371341; 11463916 [H]