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The map label for this gene is gla [H]

Identifier: 225861325

GI number: 225861325

Start: 1359939

End: 1360808

Strand: Reverse

Name: gla [H]

Synonym: SPT_1428

Alternate gene names: 225861325

Gene position: 1360808-1359939 (Counterclockwise)

Preceding gene: 225861326

Following gene: 225861324

Centisome position: 64.43

GC content: 44.02

Gene sequence:

>870_bases
ATGGATTTCACATGGGCACTGAAGTATGCCACTGAATTTTTGGGAACTGCCATTTTGATCATTCTTGGGAATGGTGCAGT
TGCCAACGTTGAACTTAAAGGTACGAAAGGTCACCAAAGTGGCTGGATCGTCATCGCTGTTGGTTATGGTATGGGGGTTA
TGATCCCAGCCTTGATGTTTGGTAACGTATCTGGGAATCACATCAACCCTGCTTTCACTCTAGGGCTTGCAGTTAGCGGT
CTTTTCCCTTGGGCACAAGTGGTACCTTACATTATCGCGCAAGTCTTGGGGGCTATCTTTGGCCAAGCCTTAGTTGTGGC
AACATACCGTCCATTCTACTTGAAAACTGAAAAACCAAATAACATCTTGGGAACTTTCTCAACTATTTCAAGTATTGACC
ATGGTACAAAAGAAAGTCGCTATGCAGCAACTGTCAATGGTTTGATTAATGAGTTTGTTGGTTCATTTGTTTTGTTCTTT
GCAGCTCTTGGTTTGACTAAAAACTTCTTTGGTGCTGAAGTGCTTCAATTCATGAAACAAAAGGCAATAGAAGCAGGACA
AACAGTTGATTTTTCTGACTTGGCTATTAAAGCACAGGTGGCTCCACACACTGCTTCAGGACTTTCTGTGGCTCACTTGG
CACTTGGATTCCTCGTTATGGCTTTGGTAACATCACTTGGAGGACCTACAGGACCTGCCTTGAACCCAGCCCGTGACTTG
GGACCACGTCTCCTTCATGCTTTCCTTCCAAAATCAGTTCTTGGTGAGCATAAAGGCGATTCAAAATGGTGGTATTCTTG
GGTACCAGTAGTAGCACCTATCGCAGCAGCAATTGCGGCAGTAGCTGTATTCAAATTCCTTTATCTCTAA

Upstream 100 bases:

>100_bases
ATCCTATTTTTTTAAAAATATTTCCAGAAATCAGTTGCTAAATAGGGTAAAAATTGATAAAATAAGGAATATCTAAAAAA
TTTTAAGGAGAATCTAGCAA

Downstream 100 bases:

>100_bases
GAAATAGCTCCTTTAACATTTGAGTGAGCACCATCTATAAGTAAGAGAGGATCAGACTGGTTCTCTCTTTTTGATTTTTT
AGGGAAATGAAAGAAACTCT

Product: glycerol facilitator-aquaporin gla (Aquaglyceroporin)(Glyceroaquaporin)

Products: glycerol [Cytoplasm] [C]

Alternate protein names: Aquaglyceroporin; Glyceroaquaporin [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL

Sequences:

>Translated_289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
>Mature_289_residues
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSG
LFPWAQVVPYIIAQVLGAIFGQALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL

Specific function: Mixed channel protein that transports both water and glycerol [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=287, Percent_Identity=28.9198606271777, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI157266307, Length=268, Percent_Identity=29.8507462686567, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI22538420, Length=287, Percent_Identity=29.616724738676, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1790362, Length=276, Percent_Identity=30.7971014492754, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558372, Length=289, Percent_Identity=26.2975778546713, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17531431, Length=281, Percent_Identity=26.6903914590747, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17531429, Length=282, Percent_Identity=26.9503546099291, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321054, Length=238, Percent_Identity=30.672268907563, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 30698; Mature: 30698

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00221 MIP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
GNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQALVVATYRPFYLKTEKPN
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCC
NILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFFAALGLTKNFFGAEVLQFMKQ
CCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
HHHHCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
HHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIVIAVGYGMGVMIPALMF
CCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCCCCCEEEEEECCCHHHHHHHHHH
GNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQALVVATYRPFYLKTEKPN
CCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEECCCCC
NILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFFAALGLTKNFFGAEVLQFMKQ
CCEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFLVMALVTSLGGPTGPALNPARDL
HHHHCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
GPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAAAIAAVAVFKFLYL
HHHHHHHHCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]