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The map label for this gene is punA [H]

Identifier: 225861272

GI number: 225861272

Start: 1308235

End: 1309044

Strand: Reverse

Name: punA [H]

Synonym: SPT_1371

Alternate gene names: 225861272

Gene position: 1309044-1308235 (Counterclockwise)

Preceding gene: 225861273

Following gene: 225861271

Centisome position: 61.98

GC content: 44.07

Gene sequence:

>810_bases
ATGACATTTTTAAACAAAATCCATGAAACTGCTACTTTCCTGAAAGAAAAGGGAATTGCAGCCCCTGAGTTCGGTCTAAT
CCTTGGATCAGGACTTGGAGAATTGGCAGAAGAAATCGAAAATCCAGTTGTAGTAGACTATGCTGAGATTCCAAACTGGG
GCCGTTCAACAGTAGTCGGTCATGCTGGTAAATTGGTATATGGTGAACTGGCAGGTCGCAAGGTCTTGGCTCTTCAAGGT
CGTTTCCATTTCTATGAAGGGAATCCTCTGGAAGTGGTGACTTTCCCAGTTCGTGTGATGAAAGTTCTTGGATGTGAAGG
TGTTATTGTAACCAATGCAGCTGGCGGTATCAGATTTGGTCCTGGTACCTTGATGGCTATCTCAGACCATATCAACATGA
CGGGGCAAAATCCATTGATGGGTGAAAACTTGGATGACTTTGGCCCACGTTTCCCAGATATGTCTAGGGCCTACACACCA
GAATACCGTGCCACTGCCCATGAAGTGGCTAAAAAACTTAATATCAAGCTTGATGAAGGTGTCTATATCGGAGTTACTGG
TCCGACTTATGAAACACCAGCAGAAATTCGTTCCTATAAGACACTGGGAGCAGATGCAGTTGGTATGTCTACGGTTCCTG
AAGTTATCGTGGCAGCCCACTCTGGCTTGAAAGTTCTGGGAATTTCATGTATCACTAACTTTGCGGCTGGTTTCCAAGAA
GAACTCAATCACGAAGAAGTTGTAGAAGTGACTGAACGTGTTAAAGGTGATTTCAAAGGCTTGCTTAAAGCGATTCTTGC
TGAATTGTAA

Upstream 100 bases:

>100_bases
CTTGCTGAAGGTGCCTTTCTACCGCCACATTACTATTCGTAATGCTAAAACCTTTGACAAAATTGGTCAAATGCTAAAAA
AATAATAAAGGAGACACACA

Downstream 100 bases:

>100_bases
GAAAAAAGATTTAAAAGGGGTAGTGTCTCTGTCTTTTCAGGATTGACTGCCTATCCGTATTAAAGAAGAAACAGAGGAAT
ACTATGAGCTTCTTCCTGCT

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: Inosine phosphorylase; Purine nucleoside phosphorylase I; PNP I; PU-NPase I [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQG
RFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQE
ELNHEEVVEVTERVKGDFKGLLKAILAEL

Sequences:

>Translated_269_residues
MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQG
RFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQE
ELNHEEVVEVTERVKGDFKGLLKAILAEL
>Mature_268_residues
TFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGR
FHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTPE
YRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQEE
LNHEEVVEVTERVKGDFKGLLKAILAEL

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=270, Percent_Identity=48.1481481481481, Blast_Score=234, Evalue=8e-62,
Organism=Homo sapiens, GI47132622, Length=248, Percent_Identity=29.0322580645161, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788746, Length=251, Percent_Identity=45.8167330677291, Blast_Score=219, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17541190, Length=260, Percent_Identity=43.0769230769231, Blast_Score=202, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323238, Length=283, Percent_Identity=42.756183745583, Blast_Score=195, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45552887, Length=275, Percent_Identity=44.7272727272727, Blast_Score=223, Evalue=1e-58,
Organism=Drosophila melanogaster, GI45552885, Length=275, Percent_Identity=44.7272727272727, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656090, Length=275, Percent_Identity=44.7272727272727, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24656093, Length=275, Percent_Identity=44.7272727272727, Blast_Score=222, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24762376, Length=239, Percent_Identity=35.9832635983264, Blast_Score=135, Evalue=3e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011270
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 29004; Mature: 28873

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVG
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCC
HAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFG
CCCCEEEEHHCCCEEEEEECEEEEECCCCEEEEEHHHHHHHHHCCCCEEEECCCCCEEEC
PGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKLNIKLDEG
CCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCC
VYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQE
EEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELNHEEVVEVTERVKGDFKGLLKAILAEL
HCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVG
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHCCCEEEEHHHCCCCCCCCCCC
HAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIRFG
CCCCEEEEHHCCCEEEEEECEEEEECCCCEEEEEHHHHHHHHHCCCCEEEECCCCCEEEC
PGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKLNIKLDEG
CCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEECCC
VYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQE
EEEEECCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
ELNHEEVVEVTERVKGDFKGLLKAILAEL
HCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10537218; 8969508; 9384377; 1629150 [H]