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The map label for this gene is ply [H]

Identifier: 225861269

GI number: 225861269

Start: 1306242

End: 1306754

Strand: Reverse

Name: ply [H]

Synonym: SPT_1368

Alternate gene names: 225861269

Gene position: 1306754-1306242 (Counterclockwise)

Preceding gene: 225861270

Following gene: 225861268

Centisome position: 61.87

GC content: 41.72

Gene sequence:

>513_bases
GTGTCAGCACAAATTACGATTAACCATAAAAAAGCGCGCTATGTTCGGATTGAGCTAGAAGGCTATAATGCCCTCAGTCT
TGCAGAAGTTGAAGTTTTCCGCTTTATAGCTACGAATGCTGAAACGGCGACACAAGTTTCTAAGCCAGTTCAACCAATCA
GTCAGACTCCTGTGAAAGATAAAACATTGACAATTCAACACAGTGGAGCTTACATTGCCCGCTACTCCATAACTTGGGAA
GAAGTTCCAGTAGATAAAGATGGAAACCAAGTTGTTCGTAGTCATTCTTGGGAAGGAAACGGTTGCAACCAGACTGCAGG
TTTTGTCCTCAACCTCCCAATCAAAGAAAATATGAGAAATCTGCGAGTTAAGATTGAGAAAAAGACGGGCCTACTATGGA
ATAGATGGCAAACAATCTATGAAAACAGACCAATTTTAGCTCAACCCCACCGTAAAATTACCCATTGGGGTACGACATTG
AATTCCAAGGTGAGTGACGATGATGTCTTGTAA

Upstream 100 bases:

>100_bases
AACAGACACTGCCCAGGATAGATTGGCAAACTTTGATGTCATTCTTTTAGACAGTTCTGGTAAAGAAATTGAGTGAAAAC
GTATAATATCTCCTAAAGAT

Downstream 100 bases:

>100_bases
TCTGATGGTAGAATGACAGTTAGTTTGTCTAGTTTATAAGAAAGTACTACCTGAGCTTGAATAGAACTCAGGTAGCTCTC
TATGAAAGAACAAAATTAAT

Product: hemolysin-related protein

Products: NA

Alternate protein names: Thiol-activated cytolysin [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWE
EVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
NSKVSDDDVL

Sequences:

>Translated_170_residues
MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWE
EVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
NSKVSDDDVL
>Mature_169_residues
SAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEE
VPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLN
SKVSDDDVL

Specific function: Sulfhydryl-activated toxin that causes cytolysis by forming pores in cholesterol containing host membranes. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation o

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Host cell membrane; Multi-pass membrane protein (By similarity). Note=Secreted as soluble protein that then inserts into the host cell membrane and forms pores formed by transmembrane beta-strands (By similarity) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiol-activated cytolysin family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001869 [H]

Pfam domain/function: PF01289 Thiol_cytolysin [H]

EC number: NA

Molecular weight: Translated: 19412; Mature: 19281

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKD
CCCEEEEECCCEEEEEEEECCCCEEEHHHHHEEHHHHCCCHHHHHHHCCCCCCCCCCCCC
KTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENMRN
CEEEEEECCCEEEEEEEEHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCHHHCCCE
LRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLNSKVSDDDVL
EEEEEECCCCCCHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETATQVSKPVQPISQTPVKD
CCEEEEECCCEEEEEEEECCCCEEEHHHHHEEHHHHCCCHHHHHHHCCCCCCCCCCCCC
KTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGCNQTAGFVLNLPIKENMRN
CEEEEEECCCEEEEEEEEHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEEEECCHHHCCCE
LRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTLNSKVSDDDVL
EEEEEECCCCCCHHHHHHHHCCCCEEECCCCEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3552992 [H]