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The map label for this gene is insK [H]

Identifier: 225861255

GI number: 225861255

Start: 1293142

End: 1293975

Strand: Reverse

Name: insK [H]

Synonym: SPT_1353

Alternate gene names: 225861255

Gene position: 1293975-1293142 (Counterclockwise)

Preceding gene: 225861256

Following gene: 225861253

Centisome position: 61.26

GC content: 36.93

Gene sequence:

>834_bases
ATGACTGAGTTTTCGTTAGTTCTTCTTCTAAAATCCATTAAACTAGCTCGTTGGACCTACTACTATCACTTGAAACAGCT
AGACAAAACAGATAAGGACCAAGAGCTTAAAGCTGAAATTCAATCCATTTTTATCGAACACAAGGGAAATTATGCTTATC
GCCGGGTTCATTTAGAACTAAGAAATCGTGCTTATCTGGTAAATCATAAAAGAGTTCAAGGCTTGATAAAAGTACTCAAT
TTACAAGCTAAAATGCGACAGAAACGAAAATATTCTTCTCATAAAGGAGACGTTGGCAAGAAGGCAGAGAATCTCATTCA
AGGCCAATTTGAAGGCTCTAAAACAATGGAAAAGTGCTACACAGATGTGACAGAATTTGCCATTCCAGCAAGTACTCAAA
AGCTTTACTTACCACCAGTTTTAGATGGCTTTAACAGTGAAATTATTGCTTTTAATCTTTCTTGTTCGCCTAATTTAGAA
CAAGTACAAACAATGTTGGAACAGGCATTCAAAGAGAAGCACTACGAGAATACGATTCTCCATAGTGACCAAGGCTGGCA
ATATCAACACGATTCTTATCATCGGTTCCTAGAGAGTAAGGGAATTCAAGCATCCATGTCACGTAAGGGTAACAGCCCAG
ACAATGGTATGATGGAGTCCTTCTTTGGGATTCTGAAATCGGAAATGTTTTACGGTTATGAGAAGTCGTTTCAGTCGTTT
AAGCAATTGGAACAAGCTATTATAGACTATATTGATTACTACAACAATAAACGAATTAAGGTAAAACTAAAAGGACTTAG
CCCTGTGCAATACAGAACTAAATCCTTCGGATAA

Upstream 100 bases:

>100_bases
AGTACCTGAGAGCGGAGAATGCCATCCTAAAAAAGTTAAGAGAACTCCGATTGAAGGAGGAAAAAGAGAAAGAAGAAAGA
CAGAAATTGTTCAAGAATTA

Downstream 100 bases:

>100_bases
ATTAATTGTCTAACTTTTGGGGTGCAGTTCACAGAACTGGCATTTTATTTTTTAGGATCTTGATGGGTTAGGTTCAATCC
CCAAGGGACCAAATTTTCAG

Product: integrase core domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLN
LQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCSPNLE
QVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSF
KQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG

Sequences:

>Translated_277_residues
MTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLN
LQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCSPNLE
QVQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSF
KQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG
>Mature_276_residues
TEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLELRNRAYLVNHKRVQGLIKVLNL
QAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCYTDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCSPNLEQ
VQTMLEQAFKEKHYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSFK
QLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG

Specific function: Involved in the transposition of the insertion sequence IS150 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=235, Percent_Identity=28.936170212766, Blast_Score=92, Evalue=6e-19,
Organism=Escherichia coli, GI1789981, Length=274, Percent_Identity=46.7153284671533, Blast_Score=252, Evalue=2e-68,
Organism=Escherichia coli, GI1788405, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787263, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786753, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786570, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1786490, Length=282, Percent_Identity=28.0141843971631, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32538; Mature: 32407

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEH
RNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCY
HCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
TDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCSPNLEQVQTMLEQAFKEKHYENTIL
HHHHHHCCCCCCCEEECCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
HSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSF
ECCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCC
>Mature Secondary Structure 
TEFSLVLLLKSIKLARWTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNYAYRRVHLEL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEEEEEH
RNRAYLVNHKRVQGLIKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQFEGSKTMEKCY
HCCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
TDVTEFAIPASTQKLYLPPVLDGFNSEIIAFNLSCSPNLEQVQTMLEQAFKEKHYENTIL
HHHHHHCCCCCCCEEECCHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEE
HSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKSFQSF
ECCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQLEQAIIDYIDYYNNKRIKVKLKGLSPVQYRTKSFG
HHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2841644; 2541046; 8041620; 9278503 [H]